Crosslinked chitosan resin is a new type of billrubin adsorbent.It can adsorb boththe conjugated and unconjugated bilirubin- Adsorption experiments were performed byadding the resins to previously prepared bilirubin s...Crosslinked chitosan resin is a new type of billrubin adsorbent.It can adsorb boththe conjugated and unconjugated bilirubin- Adsorption experiments were performed byadding the resins to previously prepared bilirubin solution, with different concentrations ofbilirubin and BSA. The dissociation equilibrium constant and conJugated free energy for thebilirubin-albumin complex were obtained according to plot.展开更多
Drug-receptor interaction plays an important role in a series of biological effects, such as cell pro- liferation, immune response, tumor metastasis, and drug delivery. Therefore, the research on drug-re- ceptor inter...Drug-receptor interaction plays an important role in a series of biological effects, such as cell pro- liferation, immune response, tumor metastasis, and drug delivery. Therefore, the research on drug-re- ceptor interaction is growing rapidly. The equilibrium dissociation constant （KD） is the basic parameter to evaluate the binding property of the drug-receptor. Thus, a variety of analytical methods have been established to determine the KD values, including radioligand binding assay, surface plasmon resonance method, fluorescence energy resonance transfer method, affinity chromatography, and isothermal ti- tration calorimetry. With the invention and innovation of new technology and analysis method, there is a deep exploration and comprehension about drug-receptor interaction. This review discusses the differ- ent methods of determining the KD values, and analyzes the applicability and the characteristic of each analytical method. Conclusively, the aim is to provide the guidance for researchers to utilize the most appropriate analytical tool to determine the KD values.展开更多
Twenty four male guinea-pigs were randomly divided into 3 groups: Group A, allergic asthmaguinea-pigs prepared by peritoneal injection and spraying inhalation of albumin; Group B, allergic asthmaguinea-pigs treated wi...Twenty four male guinea-pigs were randomly divided into 3 groups: Group A, allergic asthmaguinea-pigs prepared by peritoneal injection and spraying inhalation of albumin; Group B, allergic asthmaguinea-pigs treated with electroacupuncture through stimulating the acupoint Feishu (UB13 ) and Group C, thecontrol group. With[3H] -DHA as a radioligand, pulmonary adrenoceptor ( AC) in the 3 groups of guinea-pigs were detrermined by radioligand binding assay technique. The results showed that: ( 1 ) The maximumbinding volume of receptor ligand ( Bmax, fmol/mg prot. ) of pulmonary AC in Group A (123. 86 42. 91 )was significantly lower than that in group C (199 . 54 37. 03 , P< 0. 05) , while the difference between groupB ( 142. 00 42. 91 ) and group C was not significant ( P > 0. 05 ) . ( 2 ) The Kd ( nM) of pulmonary AC ingroup A, B and C were 5 . 10 1 . 39 , 6. 53 2 . 41 and 8. 72 2 . 02 respectively, and the differences amongthe three groups were not significant ( P >0. 05) . The results indicated that the decrease of the content anddysfunction of pulmonary AC in allergic asthma guinea-pigs might be regulated by electroacupuncture thera-py.展开更多
The high temperature gas occurs behind shock or near the wall surface of vehicle in the hypersonic flight. As the temperature exceeds 2 000 K, 4 000 K, respectively, O2 and N2 molecules are successively dissociated. B...The high temperature gas occurs behind shock or near the wall surface of vehicle in the hypersonic flight. As the temperature exceeds 2 000 K, 4 000 K, respectively, O2 and N2 molecules are successively dissociated. Because of variable components at dif- ferent temperatures and pressures, the dissociated air is no longer a perfect gas, In this paper, a new method is developed to calculate accurate thermal physical parameters with the dissociation degree providing the thermochemical equilibrium procedure. Based on the dissociation degree, it is concluded that few numbers of equations and the solutions are easily obtained. In addition, a set of formulas relating the parameter to the dissociation degree are set up four-species, O2 molecule The thermodynamic properties of dissociated air containing and N2 molecule, O atom and N atom, are studied with the new method, and the results are consistent with those with the traditional equilibrium constant method. It is shown that this method is reliable for solving thermal physical parameters easily and directly.展开更多
文摘Crosslinked chitosan resin is a new type of billrubin adsorbent.It can adsorb boththe conjugated and unconjugated bilirubin- Adsorption experiments were performed byadding the resins to previously prepared bilirubin solution, with different concentrations ofbilirubin and BSA. The dissociation equilibrium constant and conJugated free energy for thebilirubin-albumin complex were obtained according to plot.
文摘Drug-receptor interaction plays an important role in a series of biological effects, such as cell pro- liferation, immune response, tumor metastasis, and drug delivery. Therefore, the research on drug-re- ceptor interaction is growing rapidly. The equilibrium dissociation constant （KD） is the basic parameter to evaluate the binding property of the drug-receptor. Thus, a variety of analytical methods have been established to determine the KD values, including radioligand binding assay, surface plasmon resonance method, fluorescence energy resonance transfer method, affinity chromatography, and isothermal ti- tration calorimetry. With the invention and innovation of new technology and analysis method, there is a deep exploration and comprehension about drug-receptor interaction. This review discusses the differ- ent methods of determining the KD values, and analyzes the applicability and the characteristic of each analytical method. Conclusively, the aim is to provide the guidance for researchers to utilize the most appropriate analytical tool to determine the KD values.
文摘Twenty four male guinea-pigs were randomly divided into 3 groups: Group A, allergic asthmaguinea-pigs prepared by peritoneal injection and spraying inhalation of albumin; Group B, allergic asthmaguinea-pigs treated with electroacupuncture through stimulating the acupoint Feishu (UB13 ) and Group C, thecontrol group. With[3H] -DHA as a radioligand, pulmonary adrenoceptor ( AC) in the 3 groups of guinea-pigs were detrermined by radioligand binding assay technique. The results showed that: ( 1 ) The maximumbinding volume of receptor ligand ( Bmax, fmol/mg prot. ) of pulmonary AC in Group A (123. 86 42. 91 )was significantly lower than that in group C (199 . 54 37. 03 , P< 0. 05) , while the difference between groupB ( 142. 00 42. 91 ) and group C was not significant ( P > 0. 05 ) . ( 2 ) The Kd ( nM) of pulmonary AC ingroup A, B and C were 5 . 10 1 . 39 , 6. 53 2 . 41 and 8. 72 2 . 02 respectively, and the differences amongthe three groups were not significant ( P >0. 05) . The results indicated that the decrease of the content anddysfunction of pulmonary AC in allergic asthma guinea-pigs might be regulated by electroacupuncture thera-py.
基金supported by the National Natural Science Foundation of China(Nos.11732011,11672205,and 11332007)the National Key Research and Development Program of China(No.2016YFA0401200)
文摘The high temperature gas occurs behind shock or near the wall surface of vehicle in the hypersonic flight. As the temperature exceeds 2 000 K, 4 000 K, respectively, O2 and N2 molecules are successively dissociated. Because of variable components at dif- ferent temperatures and pressures, the dissociated air is no longer a perfect gas, In this paper, a new method is developed to calculate accurate thermal physical parameters with the dissociation degree providing the thermochemical equilibrium procedure. Based on the dissociation degree, it is concluded that few numbers of equations and the solutions are easily obtained. In addition, a set of formulas relating the parameter to the dissociation degree are set up four-species, O2 molecule The thermodynamic properties of dissociated air containing and N2 molecule, O atom and N atom, are studied with the new method, and the results are consistent with those with the traditional equilibrium constant method. It is shown that this method is reliable for solving thermal physical parameters easily and directly.
