The toxic effects of Cr（Vl） on antioxidant enzymes of Oxya chinensis（Orthoptera： Acridoidae） were determined. Changes in the activities of the antioxidant enzymes superoxide dismutase（SOD）, catalase（CAT）, and...The toxic effects of Cr（Vl） on antioxidant enzymes of Oxya chinensis（Orthoptera： Acridoidae） were determined. Changes in the activities of the antioxidant enzymes superoxide dismutase（SOD）, catalase（CAT）, and guaiacol peroxidase（GPx） were measured in O. chinensis insects injected with Cr（VI）. Fifth-nymphs of O. chinensis insects were injected with Cr（VI） with different concentrations （0, 75, 150, 225, 300, 375, 450 mg/kg of body weight）. The results showed that Cr（VI） led to the change of SOD, CAT, and GPx activities at different concentrations, which revealed that： （1） The oxidative stress of SOD increased with the increase of Cr （VI） concentration. （2） With the increase of Cr （VI） concentrations, CAT activities for females increased at lower concentrations, but decreased at higher concentration range, which indicated that antioxidant system of O. chinensis was not influenced by the presence of Cr （VI）. A very similar response to Cr（VI） effect for males indicated that Cr（VI） concentrations were not high enough to damage O. chinensis in terms of CAT. （3） The GPx activity for females increased in all treatments, which revealed that the damage power of Cr（VI） was increased with the increase of Cr（Vi） concentrations in terms of GPx, but the effect was not so remarkable. There was not a consistent trend of GPx activities for males in all treatments of Cr（VI）. Cr（VI）-induced changes in antioxidant enzymes were different for SOD, CAT and GPx, of which the tendency was that activities generally changed with increase of concentrations of Cr（VI） suggesting SOD, CAT, and GPx could serve as indices of oxidative stress to some extent.展开更多
Proteolytic degradation of amyloid-β （Aβ） aggregates and clearance of Aβ- induced reactive oxygen species （ROS） have received significant attention for the treatment of Alzheimer＇s disease （AD）. However, it ...Proteolytic degradation of amyloid-β （Aβ） aggregates and clearance of Aβ- induced reactive oxygen species （ROS） have received significant attention for the treatment of Alzheimer＇s disease （AD）. However, it is difficult, and often unfeasible, to directly upregulate or transport intraceUular native enzymes. More importantly, penetration of the blood-brain barrier （BBB） has presented a major impediment. Herein, we report on the rational design of a polyoxometalate- based nanozyme with both protease-like activity for depleting A~ aggregates, and superoxide dismutase （SOD）-like activity for scavenging A[3-mediated ROS. Furthermore, this nanozyme acts as a metal chelator to remove Cu from Cu-induced Aβ oligomers. More intriguingly, the nanozyme can cross the BBB and exhibits low toxicity. This work provides new insights into the design and synthesis of inorganic nanozymes as multifunctional therapeutic agents in the treatment of AD.展开更多
[Objective] The preventive and therapeutic effects of Folium perillae extract （FPE） on hyperlipemia induced by feeding rabbit with high fat forage were observed. [Method] The high fat and cholesterol diet were adopt...[Objective] The preventive and therapeutic effects of Folium perillae extract （FPE） on hyperlipemia induced by feeding rabbit with high fat forage were observed. [Method] The high fat and cholesterol diet were adopted to create the hyperlipemia model of rabbit. The rabbits were randomly and equally divided into 5 groups which were NC（ normal control）group, HLM （high lipid model） group, LFPE, MFPE and HFPE group （low, middle, high dosage of FPE were 0.17 g/kg, 0.5 g/kg and 1.5 g/kg respectively）. The contents of TC, TG, LDL-C, HDL-C, MDA and the activity of SOD in serum were detected respectively. [ Result] The three FPE groups could decrease the contents of TC, TG, LDL-C, MDA significantly （P〈0.01） compared with these of HLM group, and the contents of HDL-C （P〈0.01） and the activity of SOD （P〈0.05 ） in serum was increased significantly. [Conclusion] FPE could regulate blood-fat and counteract lipid peroxidation.展开更多
A relationship between status epilepticus(SE)and oxidative stress has recently begun to be recognized.To explore whether the flavonoids extracted from licorice(LFs)have any protective effect on kainate(KA)-induced sei...A relationship between status epilepticus(SE)and oxidative stress has recently begun to be recognized.To explore whether the flavonoids extracted from licorice(LFs)have any protective effect on kainate(KA)-induced seizure in mice,we treated mice with LFs before and after KA injection.In KA-treated mice,we found that superoxide dismutase(SOD)activity decreased immediately after the onset of seizure at 1 h and then increased at 6 h.It returned to baseline 1 d after seizure and then increased again at 3,7,and 28 d,while malondialdehyde(MDA)content remained at a high level at 1 h,6 h,3 d,7 d,and 28 d,indicating a more oxidized status related to the presence of more reactive oxygen species(ROS).Treatment with LFs before KA injection reversed the seizure-induced change in SOD activity and MDA content at 1 h,6 h,3 d,7 d,and 28 d.Treatment with LFs after seizure decreased KA-induced SOD activity and MDA content at 7 and 28 d.Also,LF pre-and post-KA treatments decreased seizure-induced neuronal cell death.Subsequently,Morris water maze tests revealed that the escape latency was significantly decreased and the number of target quadrant crossings was markedly increased in the LF-treated groups.Thus,our data indicate that LFs have protective effects on seizure-induced neuronal cell death and cognitive impairment through their anti-oxidative effects.展开更多
Blueberries are rich in phenolic compounds including anthocyanins which are closely related to biological health functions.The purpose of this study was to investigate the antioxidant activity of blueberry anthocyanin...Blueberries are rich in phenolic compounds including anthocyanins which are closely related to biological health functions.The purpose of this study was to investigate the antioxidant activity of blueberry anthocyanins extracted from'Brightwell'rabbiteye blueberries in mice.After one week of adaptation,C57BL/6J healthy male mice were divided into different groups that were administered with 100,400,or 800 mg/kg blueberry anthocyanin extract(BAE),and sacrificed at different time points(0.1,0.5,1,2,4,8,or 12 h).The plasma,eyeball,intestine,liver,and adipose tissues were collected to compare their antioxidant activity,including total antioxidant capacity(T-AOC),superoxide dismutase(SOD)activity and glutathione-peroxidase(GSH-PX/GPX)content,and the oxidative stress marker malondialdehyde(MDA)level.The results showed that blueberry anthocyanins had positive concentration-dependent antioxidant activity in vivo.The greater the concentration of BAE,the higher the T-AOC value,but the lower the MDA level.The enzyme activity of SOD,the content of GSH-PX,and messenger RNA(mRNA)levels of Cu,Zn-SOD,Mn-SOD,and GPX all confirmed that BAE played an antioxidant role after digestion in mice by improving their antioxidant defense.The in vivo antioxidant activity of BAE indicated that blueberry anthocyanins could be developed into functional foods or nutraceuticals with the aim of preventing or treating oxidative stress-related diseases.展开更多
Uniconazole, as a plant growth retardant, can enhance stress tolerance in plants, possibly because of improved antioxidation defense mechanisms with higher activities of superoxide dismutase(SOD) and peroxidase(POD) e...Uniconazole, as a plant growth retardant, can enhance stress tolerance in plants, possibly because of improved antioxidation defense mechanisms with higher activities of superoxide dismutase(SOD) and peroxidase(POD) enzymes that retard lipid peroxidation and membrane deterioration. These years much attention has been focused on the responses of antioxidant system in plants to uniconazole stress, but such studies on aquatic organism are very few. Moreover, no information is available on growth and antioxidant response in marine microalgae to uniconazole. In this paper, the growth and antioxidant responses of two marine microalgal species, Platymonas helgolandica and Pavlova viridis, at six uniconazole concentrations(0-15 mg L-1) were investigated. The results demonstrated that 3 mg L-1 uniconazole could increase significantly chlorophyll a and carbohydrate contents of P. helgolandica(P < 0.05). Higher concentrations(≥12 mg L-1) of uniconazole could inhibit significantly the growth, dry weight, chlorophyll-a and carbohydrate contents of P. helgolandica and P. viridis(P < 0.05). Uniconazole caused a significant increase in lipid peroxidation production(MDA) at higher concentrations(≥ 9 mg L-1). The activities of antioxidant enzymes, superoxide dismutase(SOD) and catalase(CAT) were enhanced remarkably at low concentrations of uniconazole. However, significant reduction of SOD and CAT activities was observed at higher concentrations of uniconazole.展开更多
Cerium oxide nanoparticles （CONPs）, widely used in catalytic applications owing to their robust redox reaction, are now being considered in therapeutic applications based on their enzyme mimetic properties such as c...Cerium oxide nanoparticles （CONPs）, widely used in catalytic applications owing to their robust redox reaction, are now being considered in therapeutic applications based on their enzyme mimetic properties such as catalase and super oxide dismutase （SOD） mimetic activities. In therapeutic applications, the emerging demand for CONPs with low cytotoxicity, high cost efficiency, and high enzyme mimetic capability necessitates the exploration of alternative synthesis and effective material design. This study presents a room temperature aqueous synthesis for low-cost production of shape-selective CONPs without potentially harmful organic substances, and additionally, investigates cell viability and catalase and SOD mimetic activities. This synthesis, at room temperature, produced CONPs with particular planes： {111}/{100} nanopolyhedra, {100} nano/submicron cubes, and {111}/{100} nanorods that grew in [110] longitudinal direction. Enzymatic activity assays indicated that nanopolyhedra with a high concentration of Ce4＋ ions promoted catalase mimetic activity, while nanocubes and nanorods with high Ce3＋ ion concentrations enhanced SOD mimetic activity. This is the first study indicating that shape and facet configuration design of CONPs, coupled with the retention of dominant, specific Ce valence states, potentiates enzyme mimetic activities. These findings may be utilized for CONP design aimed at enhancing enzyme mimetic activities in therapeutic applications.展开更多
Moxibustion at Shenque (CV 8) for two weeks increased the activity of both total antioxidation and SOD in rat plasma and gastric mucosa, decreased the content of MDA in the plasma butnot in the gastric mucosa.
In the present paper, blood lipids peroxide(LPO) level and activities of glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) were investigated before and after combined treatment of acupuncture and moxibustio...In the present paper, blood lipids peroxide(LPO) level and activities of glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) were investigated before and after combined treatment of acupuncture and moxibustion and Chinese drugs in patients of vascular dementia(VD), and their results were compared with those in healthy persons with the similar ages to the patients. The results showed that the blood LPO level increased significantly, and the activities of SOD and GSH-Px reduced significantly in patients of VD as compared with those in the control group. Degrees of patient’s condition were related with amplitudes of the increase of LPO and the reduction of activities of GSHPx and SOD. Combined treatment of acupuncture and moxibustion and Chinese drugs could raise markedly activitles of blood GSH-Px and SOD, and lowered LPO level in the patients of VD, which are related to clinical therapeutic effects. It is considered that combination of acupuncture and moxibustion with Chinese drugs can increase the action of the antiperoxidative system in the patients of VD, exerting anti-peroxidative ability and clearing LPO and reducing the oxidative injury of the organism by oxygen free radical, which is one of mechanisms of combined treatment of acupuncture and moxibustion with Chinese drugs.展开更多
Objective To rurther conflrm the role or lipld-peroxldation caused by oxygen free radicals injury played in the pathogenesis of dllsted cardlomypathy. MethOds The superoxide dismutase activities and lipids composltion...Objective To rurther conflrm the role or lipld-peroxldation caused by oxygen free radicals injury played in the pathogenesis of dllsted cardlomypathy. MethOds The superoxide dismutase activities and lipids composltion of erythrocytes in 18 patients with dilated cardiomyopathy and 16 healthy controls were measured. Results Superoxide dlsmutase(SOD) activites of erythrocytes were lower in dilated cardiomyopathy(DCM) patients than that in healthy controls (P <o. oo1 ). The lipids composition of erythrocytes has changed in the DCM patients compared with healthy controls: total liplds changed little (P >o. o5); total phospholipids were lower, but not significantly (P>o. o5): total cholesterol increased significantly (P <o. o5). The cholesterol to phospholipids molecular ratio of erythrocyte membrane has increased remarkably (P >o. o5). Conclusion It can be supposed that decreased SOD activitles play an important role in the damage or membrane system and the pathogensis of DCM.展开更多
Objective:To assess the role of oxidative stress on anaemia in pregnancy.Methods:Blood samples were collected from pregnant and non-pregnant women who came for antenatal clinic and medical check at Comprehensive Healt...Objective:To assess the role of oxidative stress on anaemia in pregnancy.Methods:Blood samples were collected from pregnant and non-pregnant women who came for antenatal clinic and medical check at Comprehensive Health Center,Akungba-Akoko and Iwaro General Hospital in Akoko Area of Ondo State,Nigeria.Thick and thin blood films were prepared and used for malaria parasite counts.Haemoglobin level was determined by colorimetric method using Drabkin’s solution.Oxidative status was determined using malondiadelhyde level as an indicator of lipid peroxidation,while ascorbic acid and reduced glutathione levels were measured by standard spectrophotometric methods.Results:Mean parasite density was significantly higher in pregnant women than non-pregnant women(P【0.05).Haemoglobin level was significantly reduced in malaria positive pregnant and non-pregnant women than malaria negative(8.3-10.0 g/dL)(P【0.05).The oxidative status indicated that malondialdehyde(MDA) was significantly increased in pregnant[(2.5±0.7) nmol/mL]than non-pregnant women[(1.8±0.1) nmol/mL] (P【0.05),while Vit C and superoxide dismutase(SOD) levels were significantly reduced in pregnant than non-pregnant women(P【0.05).There was an inverse correlation between Hb and MDA levels in pregnant women studied.Positive correlation was observed between the mean MDA level and parasite density(r = 0.53).The Hb level decreased as the parasite density and MDA level increased in pregnant women.Conclusions:This study shows that oxidative stress, caused by malaria infection could be part of the contributing factors responsible for anaemia in pregnancy.展开更多
基金The National Natural Science Foundation of China(No.30170612 30470219) International Cooperation of Shanxi Province(No.041005)
文摘The toxic effects of Cr（Vl） on antioxidant enzymes of Oxya chinensis（Orthoptera： Acridoidae） were determined. Changes in the activities of the antioxidant enzymes superoxide dismutase（SOD）, catalase（CAT）, and guaiacol peroxidase（GPx） were measured in O. chinensis insects injected with Cr（VI）. Fifth-nymphs of O. chinensis insects were injected with Cr（VI） with different concentrations （0, 75, 150, 225, 300, 375, 450 mg/kg of body weight）. The results showed that Cr（VI） led to the change of SOD, CAT, and GPx activities at different concentrations, which revealed that： （1） The oxidative stress of SOD increased with the increase of Cr （VI） concentration. （2） With the increase of Cr （VI） concentrations, CAT activities for females increased at lower concentrations, but decreased at higher concentration range, which indicated that antioxidant system of O. chinensis was not influenced by the presence of Cr （VI）. A very similar response to Cr（VI） effect for males indicated that Cr（VI） concentrations were not high enough to damage O. chinensis in terms of CAT. （3） The GPx activity for females increased in all treatments, which revealed that the damage power of Cr（VI） was increased with the increase of Cr（Vi） concentrations in terms of GPx, but the effect was not so remarkable. There was not a consistent trend of GPx activities for males in all treatments of Cr（VI）. Cr（VI）-induced changes in antioxidant enzymes were different for SOD, CAT and GPx, of which the tendency was that activities generally changed with increase of concentrations of Cr（VI） suggesting SOD, CAT, and GPx could serve as indices of oxidative stress to some extent.
基金Financial support was provided by the National Basic Research Program of China （973 Project） （Nos. 2011CB936004 and 2012CB720602） and the National Natural Science Foundation of China （Nos. 21210002, 21431007, 21402183, and 21533008）.
文摘Proteolytic degradation of amyloid-β （Aβ） aggregates and clearance of Aβ- induced reactive oxygen species （ROS） have received significant attention for the treatment of Alzheimer＇s disease （AD）. However, it is difficult, and often unfeasible, to directly upregulate or transport intraceUular native enzymes. More importantly, penetration of the blood-brain barrier （BBB） has presented a major impediment. Herein, we report on the rational design of a polyoxometalate- based nanozyme with both protease-like activity for depleting A~ aggregates, and superoxide dismutase （SOD）-like activity for scavenging A[3-mediated ROS. Furthermore, this nanozyme acts as a metal chelator to remove Cu from Cu-induced Aβ oligomers. More intriguingly, the nanozyme can cross the BBB and exhibits low toxicity. This work provides new insights into the design and synthesis of inorganic nanozymes as multifunctional therapeutic agents in the treatment of AD.
基金Supported by the National Natural Science Foundation of China(10572029,1063201,10772054)~~
文摘[Objective] The preventive and therapeutic effects of Folium perillae extract （FPE） on hyperlipemia induced by feeding rabbit with high fat forage were observed. [Method] The high fat and cholesterol diet were adopted to create the hyperlipemia model of rabbit. The rabbits were randomly and equally divided into 5 groups which were NC（ normal control）group, HLM （high lipid model） group, LFPE, MFPE and HFPE group （low, middle, high dosage of FPE were 0.17 g/kg, 0.5 g/kg and 1.5 g/kg respectively）. The contents of TC, TG, LDL-C, HDL-C, MDA and the activity of SOD in serum were detected respectively. [ Result] The three FPE groups could decrease the contents of TC, TG, LDL-C, MDA significantly （P〈0.01） compared with these of HLM group, and the contents of HDL-C （P〈0.01） and the activity of SOD （P〈0.05 ） in serum was increased significantly. [Conclusion] FPE could regulate blood-fat and counteract lipid peroxidation.
基金Project supported by the Scientific Research Foundation for Returned Scholars of the Ministry of Education of China(2011)the Project of Experiment Animal Platform of Department of Science and Technology of Zhejiang Province(No.2013C37026)the Hangzhou Science and Technology Development Plan(No.20100333T24),China
文摘A relationship between status epilepticus(SE)and oxidative stress has recently begun to be recognized.To explore whether the flavonoids extracted from licorice(LFs)have any protective effect on kainate(KA)-induced seizure in mice,we treated mice with LFs before and after KA injection.In KA-treated mice,we found that superoxide dismutase(SOD)activity decreased immediately after the onset of seizure at 1 h and then increased at 6 h.It returned to baseline 1 d after seizure and then increased again at 3,7,and 28 d,while malondialdehyde(MDA)content remained at a high level at 1 h,6 h,3 d,7 d,and 28 d,indicating a more oxidized status related to the presence of more reactive oxygen species(ROS).Treatment with LFs before KA injection reversed the seizure-induced change in SOD activity and MDA content at 1 h,6 h,3 d,7 d,and 28 d.Treatment with LFs after seizure decreased KA-induced SOD activity and MDA content at 7 and 28 d.Also,LF pre-and post-KA treatments decreased seizure-induced neuronal cell death.Subsequently,Morris water maze tests revealed that the escape latency was significantly decreased and the number of target quadrant crossings was markedly increased in the LF-treated groups.Thus,our data indicate that LFs have protective effects on seizure-induced neuronal cell death and cognitive impairment through their anti-oxidative effects.
基金supported by the Jiangsu Provincial Key Research and Development Program(No.BE2021705),China。
文摘Blueberries are rich in phenolic compounds including anthocyanins which are closely related to biological health functions.The purpose of this study was to investigate the antioxidant activity of blueberry anthocyanins extracted from'Brightwell'rabbiteye blueberries in mice.After one week of adaptation,C57BL/6J healthy male mice were divided into different groups that were administered with 100,400,or 800 mg/kg blueberry anthocyanin extract(BAE),and sacrificed at different time points(0.1,0.5,1,2,4,8,or 12 h).The plasma,eyeball,intestine,liver,and adipose tissues were collected to compare their antioxidant activity,including total antioxidant capacity(T-AOC),superoxide dismutase(SOD)activity and glutathione-peroxidase(GSH-PX/GPX)content,and the oxidative stress marker malondialdehyde(MDA)level.The results showed that blueberry anthocyanins had positive concentration-dependent antioxidant activity in vivo.The greater the concentration of BAE,the higher the T-AOC value,but the lower the MDA level.The enzyme activity of SOD,the content of GSH-PX,and messenger RNA(mRNA)levels of Cu,Zn-SOD,Mn-SOD,and GPX all confirmed that BAE played an antioxidant role after digestion in mice by improving their antioxidant defense.The in vivo antioxidant activity of BAE indicated that blueberry anthocyanins could be developed into functional foods or nutraceuticals with the aim of preventing or treating oxidative stress-related diseases.
基金supported by the National Natural Science Foundation of China (Nos. 21071133, 51273184 and 81202399)the Program for Science and Technology of Shandong Province (2011GHY11521)the Natural Science Foundation of Qingdao City (Nos. 11-2-4-1-(9) gch), 12-1-3-52-(1)-nsh and 12-1-4-16-(7)-jch)
文摘Uniconazole, as a plant growth retardant, can enhance stress tolerance in plants, possibly because of improved antioxidation defense mechanisms with higher activities of superoxide dismutase(SOD) and peroxidase(POD) enzymes that retard lipid peroxidation and membrane deterioration. These years much attention has been focused on the responses of antioxidant system in plants to uniconazole stress, but such studies on aquatic organism are very few. Moreover, no information is available on growth and antioxidant response in marine microalgae to uniconazole. In this paper, the growth and antioxidant responses of two marine microalgal species, Platymonas helgolandica and Pavlova viridis, at six uniconazole concentrations(0-15 mg L-1) were investigated. The results demonstrated that 3 mg L-1 uniconazole could increase significantly chlorophyll a and carbohydrate contents of P. helgolandica(P < 0.05). Higher concentrations(≥12 mg L-1) of uniconazole could inhibit significantly the growth, dry weight, chlorophyll-a and carbohydrate contents of P. helgolandica and P. viridis(P < 0.05). Uniconazole caused a significant increase in lipid peroxidation production(MDA) at higher concentrations(≥ 9 mg L-1). The activities of antioxidant enzymes, superoxide dismutase(SOD) and catalase(CAT) were enhanced remarkably at low concentrations of uniconazole. However, significant reduction of SOD and CAT activities was observed at higher concentrations of uniconazole.
文摘Cerium oxide nanoparticles （CONPs）, widely used in catalytic applications owing to their robust redox reaction, are now being considered in therapeutic applications based on their enzyme mimetic properties such as catalase and super oxide dismutase （SOD） mimetic activities. In therapeutic applications, the emerging demand for CONPs with low cytotoxicity, high cost efficiency, and high enzyme mimetic capability necessitates the exploration of alternative synthesis and effective material design. This study presents a room temperature aqueous synthesis for low-cost production of shape-selective CONPs without potentially harmful organic substances, and additionally, investigates cell viability and catalase and SOD mimetic activities. This synthesis, at room temperature, produced CONPs with particular planes： {111}/{100} nanopolyhedra, {100} nano/submicron cubes, and {111}/{100} nanorods that grew in [110] longitudinal direction. Enzymatic activity assays indicated that nanopolyhedra with a high concentration of Ce4＋ ions promoted catalase mimetic activity, while nanocubes and nanorods with high Ce3＋ ion concentrations enhanced SOD mimetic activity. This is the first study indicating that shape and facet configuration design of CONPs, coupled with the retention of dominant, specific Ce valence states, potentiates enzyme mimetic activities. These findings may be utilized for CONP design aimed at enhancing enzyme mimetic activities in therapeutic applications.
文摘Moxibustion at Shenque (CV 8) for two weeks increased the activity of both total antioxidation and SOD in rat plasma and gastric mucosa, decreased the content of MDA in the plasma butnot in the gastric mucosa.
文摘In the present paper, blood lipids peroxide(LPO) level and activities of glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) were investigated before and after combined treatment of acupuncture and moxibustion and Chinese drugs in patients of vascular dementia(VD), and their results were compared with those in healthy persons with the similar ages to the patients. The results showed that the blood LPO level increased significantly, and the activities of SOD and GSH-Px reduced significantly in patients of VD as compared with those in the control group. Degrees of patient’s condition were related with amplitudes of the increase of LPO and the reduction of activities of GSHPx and SOD. Combined treatment of acupuncture and moxibustion and Chinese drugs could raise markedly activitles of blood GSH-Px and SOD, and lowered LPO level in the patients of VD, which are related to clinical therapeutic effects. It is considered that combination of acupuncture and moxibustion with Chinese drugs can increase the action of the antiperoxidative system in the patients of VD, exerting anti-peroxidative ability and clearing LPO and reducing the oxidative injury of the organism by oxygen free radical, which is one of mechanisms of combined treatment of acupuncture and moxibustion with Chinese drugs.
文摘Objective To rurther conflrm the role or lipld-peroxldation caused by oxygen free radicals injury played in the pathogenesis of dllsted cardlomypathy. MethOds The superoxide dismutase activities and lipids composltion of erythrocytes in 18 patients with dilated cardiomyopathy and 16 healthy controls were measured. Results Superoxide dlsmutase(SOD) activites of erythrocytes were lower in dilated cardiomyopathy(DCM) patients than that in healthy controls (P <o. oo1 ). The lipids composition of erythrocytes has changed in the DCM patients compared with healthy controls: total liplds changed little (P >o. o5); total phospholipids were lower, but not significantly (P>o. o5): total cholesterol increased significantly (P <o. o5). The cholesterol to phospholipids molecular ratio of erythrocyte membrane has increased remarkably (P >o. o5). Conclusion It can be supposed that decreased SOD activitles play an important role in the damage or membrane system and the pathogensis of DCM.
文摘Objective:To assess the role of oxidative stress on anaemia in pregnancy.Methods:Blood samples were collected from pregnant and non-pregnant women who came for antenatal clinic and medical check at Comprehensive Health Center,Akungba-Akoko and Iwaro General Hospital in Akoko Area of Ondo State,Nigeria.Thick and thin blood films were prepared and used for malaria parasite counts.Haemoglobin level was determined by colorimetric method using Drabkin’s solution.Oxidative status was determined using malondiadelhyde level as an indicator of lipid peroxidation,while ascorbic acid and reduced glutathione levels were measured by standard spectrophotometric methods.Results:Mean parasite density was significantly higher in pregnant women than non-pregnant women(P【0.05).Haemoglobin level was significantly reduced in malaria positive pregnant and non-pregnant women than malaria negative(8.3-10.0 g/dL)(P【0.05).The oxidative status indicated that malondialdehyde(MDA) was significantly increased in pregnant[(2.5±0.7) nmol/mL]than non-pregnant women[(1.8±0.1) nmol/mL] (P【0.05),while Vit C and superoxide dismutase(SOD) levels were significantly reduced in pregnant than non-pregnant women(P【0.05).There was an inverse correlation between Hb and MDA levels in pregnant women studied.Positive correlation was observed between the mean MDA level and parasite density(r = 0.53).The Hb level decreased as the parasite density and MDA level increased in pregnant women.Conclusions:This study shows that oxidative stress, caused by malaria infection could be part of the contributing factors responsible for anaemia in pregnancy.
