Mimetic enzymes are devised as alternates or supplements of natural enzymes in broad fields but regulating their activities in a switchable manner remains challenging.Herein,we proposed an enzymatic self-assembly/disa...Mimetic enzymes are devised as alternates or supplements of natural enzymes in broad fields but regulating their activities in a switchable manner remains challenging.Herein,we proposed an enzymatic self-assembly/disassembly strategy to address this issue.A peptide molecule Nap FFEYIH(YH) was rationally designed which,after self-assembling into nanofibers,lined up the histidine moieties to form active hydrolysis centers for mimicking hydrolase activity.Enzymatic dephosphorylation of Nap FFEYp IH(Yp H) by alkaline phosphatase to yield YH also turned “ON” the hydrolase activity.In turn,phosphorylation of YH by phosphokinase epidermal growth factor receptor to yield Yp H disassembled the nanofibers and thus turned the activity “OFF”.As such,the “ON”/“OFF” of the mimetic hydrolase activities could be regulated under physiological conditions through ALP/EGFR-mediated self-assembly/disassembly of histidine nanofibers.This work provides a feasible strategy for the on-demand fabrication of artificial enzymes with controllable and superior activities.展开更多
Inspired by Niels Bohr’s adoption of the Taijitu symbol of Yin Yang in his quantum complementarity worldview, this original paper introduces Yin Yang cosmology as an original epistemic quantum information theoretic m...Inspired by Niels Bohr’s adoption of the Taijitu symbol of Yin Yang in his quantum complementarity worldview, this original paper introduces Yin Yang cosmology as an original epistemic quantum information theoretic model of the cosmos. The model is relation-based (not object-based) and is fundamentally established on foundations of positive and negative correlations between correlates. Adopting the holographic principle of ‘t Hooft, the fundamental dualistic processes of Yin Yang cosmology are interacting self-assembly (host) and self-disassembly (virus) subroutines in a superposition algorithm that cooperate in a phase portrait on a holographic boundary. This cooperative self-assembly and self-disassembly Yin Yang cosmology is formalized in a pair of differential equations that plot as orthogonal swirling Taijitu, and which together form an epistemic information-accreting unit-circle limit-cycle. Formal and natural descriptions are given for the continuously evolving saturation of quantum information entropy production under Yin Yang theoretic unit circle limit cycle dynamics. This original paper includes a short discussion on the known cosmos, from Big Bang to thermodynamic Big Chill, which is a quarter-cycle in Yin Yang cosmology. Our known thermodynamic cosmos is contextualized within a Yin Yang full-oscillation stationary wave that self-evolves, both its content (correlations between correlates) and its emergent laws, all-at-once in superposition. In a general theory of thermodynamics, the four superposed quarter-cycles of Yin Yang cosmology are: thermodynamic (the known cosmos), cryodynamic, anti-thermodynamic and anti-cryodynamic. Yin Yang cosmology guided by its epistemic limit cycle, self-evolves towards a self-referential symbolic ouroboros of quantum cosmological intelligence and generates Hawking’s ex post facto configurations for Life.展开更多
基金supported by the National Natural Science Foundation of China (22234002,22204019,82172097)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX22_0245)。
文摘Mimetic enzymes are devised as alternates or supplements of natural enzymes in broad fields but regulating their activities in a switchable manner remains challenging.Herein,we proposed an enzymatic self-assembly/disassembly strategy to address this issue.A peptide molecule Nap FFEYIH(YH) was rationally designed which,after self-assembling into nanofibers,lined up the histidine moieties to form active hydrolysis centers for mimicking hydrolase activity.Enzymatic dephosphorylation of Nap FFEYp IH(Yp H) by alkaline phosphatase to yield YH also turned “ON” the hydrolase activity.In turn,phosphorylation of YH by phosphokinase epidermal growth factor receptor to yield Yp H disassembled the nanofibers and thus turned the activity “OFF”.As such,the “ON”/“OFF” of the mimetic hydrolase activities could be regulated under physiological conditions through ALP/EGFR-mediated self-assembly/disassembly of histidine nanofibers.This work provides a feasible strategy for the on-demand fabrication of artificial enzymes with controllable and superior activities.
文摘Inspired by Niels Bohr’s adoption of the Taijitu symbol of Yin Yang in his quantum complementarity worldview, this original paper introduces Yin Yang cosmology as an original epistemic quantum information theoretic model of the cosmos. The model is relation-based (not object-based) and is fundamentally established on foundations of positive and negative correlations between correlates. Adopting the holographic principle of ‘t Hooft, the fundamental dualistic processes of Yin Yang cosmology are interacting self-assembly (host) and self-disassembly (virus) subroutines in a superposition algorithm that cooperate in a phase portrait on a holographic boundary. This cooperative self-assembly and self-disassembly Yin Yang cosmology is formalized in a pair of differential equations that plot as orthogonal swirling Taijitu, and which together form an epistemic information-accreting unit-circle limit-cycle. Formal and natural descriptions are given for the continuously evolving saturation of quantum information entropy production under Yin Yang theoretic unit circle limit cycle dynamics. This original paper includes a short discussion on the known cosmos, from Big Bang to thermodynamic Big Chill, which is a quarter-cycle in Yin Yang cosmology. Our known thermodynamic cosmos is contextualized within a Yin Yang full-oscillation stationary wave that self-evolves, both its content (correlations between correlates) and its emergent laws, all-at-once in superposition. In a general theory of thermodynamics, the four superposed quarter-cycles of Yin Yang cosmology are: thermodynamic (the known cosmos), cryodynamic, anti-thermodynamic and anti-cryodynamic. Yin Yang cosmology guided by its epistemic limit cycle, self-evolves towards a self-referential symbolic ouroboros of quantum cosmological intelligence and generates Hawking’s ex post facto configurations for Life.
