The paper reports the method applieable for determination of traces of mercury in 2.0mol·L -1 HCl 1.5 mol·L -1 NaCl 1.0μg·ml -1 Cd 2+ medium with differential pulse anoldic stripping voltammetry.Stripp...The paper reports the method applieable for determination of traces of mercury in 2.0mol·L -1 HCl 1.5 mol·L -1 NaCl 1.0μg·ml -1 Cd 2+ medium with differential pulse anoldic stripping voltammetry.Stripping peak and concentration of Hg is linear in range of 0.50～100ng·ml -1 when the deposition time is 5min.The relative standard deviation of 4.0ng·ml -1 Hg 2+ is 8.87% while n=8.After enrichment and separation of Hg in synthetic samples is obtained by using a microcolumn of sulfhydry cotton,the average recovery is about 80%.展开更多
目的:针对贝类中高毒重金属铅(Pb)和镉(Cd),开发一种可再生、廉价、快速、灵敏的电化学传感器。方法:以自制离子液体/石墨烯电极(IL/GNs HME)为工作电极,采用微分脉冲阳极溶出伏安法(DPASV)原位镀铋,根据得到的溶出峰电流,实现对Pb^(2+)...目的:针对贝类中高毒重金属铅(Pb)和镉(Cd),开发一种可再生、廉价、快速、灵敏的电化学传感器。方法:以自制离子液体/石墨烯电极(IL/GNs HME)为工作电极,采用微分脉冲阳极溶出伏安法(DPASV)原位镀铋,根据得到的溶出峰电流,实现对Pb^(2+)和Cd^(2+)的同步测定。结果:在铋离子质量浓度500μg/L,富集时间420 s,搅拌速度600 r/min,富集电压-1.1 V,缓冲体系HAc-Na Ac p H 4.2的条件下,利用电化学传感器测得的Pb^(2+)和Cd^(2+)的线性范围均为1.0~45.0μg/L,检测限分别达0.5μg/L和0.8μg/L。采用IL/GNs HME电化学传感器同时检测扇贝、褶牡蛎、长竹蛏、菲律宾蛤子中Pb^(2+)、Cd^(2+)含量时,所得结果均与电感耦合等离子体质谱(ICP-MS)检测结果相符。结论:基于IL/GNs HME构建的电化学传感器操作简便、灵敏度高,可实现对贝类中Pb^(2+)、Cd^(2+)含量的快速、同步测定。展开更多
An electrochemical method for the simultaneous detection of two different DNA sequences from PAT and FMV 35S gene sequence using CdS and PbS quantum dots （QDs） as labels was described. The QDs were readily functiona...An electrochemical method for the simultaneous detection of two different DNA sequences from PAT and FMV 35S gene sequence using CdS and PbS quantum dots （QDs） as labels was described. The QDs were readily functionalized with oligonucleotides as electrochemical DNA probes and selectively hybridized to the complementary sequences immobilized on the microplate. The QDs anchored on the hybrids were dissolved in the solution by the oxidation of HNO3 and further detected by a sensitive differential pulse anodic stripping voltammetric method （DPASV）. The DPASV signals of the oxidation of Cd^2＋ and Pb^2＋ ions present in the solution were different and reflected the identity of corresponding ssDNA targets sequences.展开更多
文摘The paper reports the method applieable for determination of traces of mercury in 2.0mol·L -1 HCl 1.5 mol·L -1 NaCl 1.0μg·ml -1 Cd 2+ medium with differential pulse anoldic stripping voltammetry.Stripping peak and concentration of Hg is linear in range of 0.50～100ng·ml -1 when the deposition time is 5min.The relative standard deviation of 4.0ng·ml -1 Hg 2+ is 8.87% while n=8.After enrichment and separation of Hg in synthetic samples is obtained by using a microcolumn of sulfhydry cotton,the average recovery is about 80%.
文摘目的:针对贝类中高毒重金属铅(Pb)和镉(Cd),开发一种可再生、廉价、快速、灵敏的电化学传感器。方法:以自制离子液体/石墨烯电极(IL/GNs HME)为工作电极,采用微分脉冲阳极溶出伏安法(DPASV)原位镀铋,根据得到的溶出峰电流,实现对Pb^(2+)和Cd^(2+)的同步测定。结果:在铋离子质量浓度500μg/L,富集时间420 s,搅拌速度600 r/min,富集电压-1.1 V,缓冲体系HAc-Na Ac p H 4.2的条件下,利用电化学传感器测得的Pb^(2+)和Cd^(2+)的线性范围均为1.0~45.0μg/L,检测限分别达0.5μg/L和0.8μg/L。采用IL/GNs HME电化学传感器同时检测扇贝、褶牡蛎、长竹蛏、菲律宾蛤子中Pb^(2+)、Cd^(2+)含量时,所得结果均与电感耦合等离子体质谱(ICP-MS)检测结果相符。结论:基于IL/GNs HME构建的电化学传感器操作简便、灵敏度高,可实现对贝类中Pb^(2+)、Cd^(2+)含量的快速、同步测定。
基金the National Natural Science Foundation of China(Nos.20405008,20635020).
文摘An electrochemical method for the simultaneous detection of two different DNA sequences from PAT and FMV 35S gene sequence using CdS and PbS quantum dots （QDs） as labels was described. The QDs were readily functionalized with oligonucleotides as electrochemical DNA probes and selectively hybridized to the complementary sequences immobilized on the microplate. The QDs anchored on the hybrids were dissolved in the solution by the oxidation of HNO3 and further detected by a sensitive differential pulse anodic stripping voltammetric method （DPASV）. The DPASV signals of the oxidation of Cd^2＋ and Pb^2＋ ions present in the solution were different and reflected the identity of corresponding ssDNA targets sequences.
