AIM: To observe the efficacy and mechanism of grain-sized moxibustion at different acupoints in a rat model of ulcerative colitis (UC). METHODS: Sprague-Dawley rats were randomly divided into control, UC model, grain-...AIM: To observe the efficacy and mechanism of grain-sized moxibustion at different acupoints in a rat model of ulcerative colitis (UC). METHODS: Sprague-Dawley rats were randomly divided into control, UC model, grain-sized moxibustion at a single acupoint (CV 12), grain-sized moxibustion at two acupoints (CV 12 and CV 4), grain-sized moxibustion at three acupoints (CV 12, CV 4, and ST 36), and medication groups (n = 8/group). The UC model was established by enema of trinitrobenzene sulfonic acid. Direct moxibustion was used once a day for 7 d. Disease activity index (DAI) was evaluated before and after the treatment. Morphologic changes of intestinal tissue were observed under an optical microscope. The expression of tumor necrosis factor (TNF)-alpha and p38 mitogen-activated protein kinase (p38MAPK) in colonic tissue was detected using Western blot, and the levels of occludin and zonula occludens-1 (ZO-1) mRNAs were detected using reverse transcription PCR. RESULTS: Compared with the control group, the intestinal mucosae were incomplete in the model group, glandular structures were irregular, and submucosae were edematous, hyperemic, and infiltrated with inflammatory cells. The DAI scores and expression of TNF-alpha and p38MAPK were increased significantly in the model group compared to controls (Ps < 0.01), while the mRNA levels of occludin and ZO-1 were reduced significantly (Ps < 0.01). Compared with the model group, colonic mucosa and the arrangement of glands were complete and regular in the treatment groups. DAI scores and the expression of TNF-alpha and p38MAPK were reduced significantly in moxibustion groups compared to controls (Ps < 0.01), while the mRNA levels of occludin and ZO-1 were increased significantly (Ps < 0.01). The improvements in the above indices in the three acupoints group and the medication group were superior to those in the single and two acupoints groups (all P < 0.05). CONCLUSION: Reduction of TNF-alpha and p38MAPK and increased expression of occludin and ZO-1 in colonic tissue rep展开更多
The observation stations of Northern China are divided into three regions:the arid Northwest China,the Loess Plateau,and the cool Northeast China.The consistencies,differences,and associated mechanisms of land surface...The observation stations of Northern China are divided into three regions:the arid Northwest China,the Loess Plateau,and the cool Northeast China.The consistencies,differences,and associated mechanisms of land surface thermal-hydrologic processes among the three regions were studied based on the normalization of major variables of land surface thermal-hydrologic processes,using data collected during prevailing summer monsoon period(July and August,2008).It is shown that differences of surface thermal-hydrologic processes are remarkable among the three regions because of different impacts of summer monsoon.Especially their soil wet layers occur at different depths,and the average albedo and its diurnal variations are distinctly different.Surface net short-wave radiation in the Loess Plateau is close to that in the cool Northeast China,but its surface net long-wave radiation is close to that in the arid Northwest China.And the ratio of net radiation to global solar radiation in the cool Northeast China is higher than the other two regions,though its temperature is lower.There are obvious regional differences in the ratios of surface sensible and latent heat fluxes to net radiation for the three regions because of distinct contribution of sensible and latent heat fluxesto land surface energy balance.The three regions are markedly different in the ratio of water vapor flux to pan evaporation,but they are consistent in the ratio of water vapor flux to precipitation.These results not only indicate different influences of climate and environmental factors on land surface thermal-hydrologic processes in the three regions,but also show that summer monsoon is important in the formation and variation of the pattern of land surface thermal-hydrologic processes.展开更多
基金Supported by Social Development Research Project of Scientific and Technological Department of Liaoning Province,ChinaNo.2012225018
文摘AIM: To observe the efficacy and mechanism of grain-sized moxibustion at different acupoints in a rat model of ulcerative colitis (UC). METHODS: Sprague-Dawley rats were randomly divided into control, UC model, grain-sized moxibustion at a single acupoint (CV 12), grain-sized moxibustion at two acupoints (CV 12 and CV 4), grain-sized moxibustion at three acupoints (CV 12, CV 4, and ST 36), and medication groups (n = 8/group). The UC model was established by enema of trinitrobenzene sulfonic acid. Direct moxibustion was used once a day for 7 d. Disease activity index (DAI) was evaluated before and after the treatment. Morphologic changes of intestinal tissue were observed under an optical microscope. The expression of tumor necrosis factor (TNF)-alpha and p38 mitogen-activated protein kinase (p38MAPK) in colonic tissue was detected using Western blot, and the levels of occludin and zonula occludens-1 (ZO-1) mRNAs were detected using reverse transcription PCR. RESULTS: Compared with the control group, the intestinal mucosae were incomplete in the model group, glandular structures were irregular, and submucosae were edematous, hyperemic, and infiltrated with inflammatory cells. The DAI scores and expression of TNF-alpha and p38MAPK were increased significantly in the model group compared to controls (Ps < 0.01), while the mRNA levels of occludin and ZO-1 were reduced significantly (Ps < 0.01). Compared with the model group, colonic mucosa and the arrangement of glands were complete and regular in the treatment groups. DAI scores and the expression of TNF-alpha and p38MAPK were reduced significantly in moxibustion groups compared to controls (Ps < 0.01), while the mRNA levels of occludin and ZO-1 were increased significantly (Ps < 0.01). The improvements in the above indices in the three acupoints group and the medication group were superior to those in the single and two acupoints groups (all P < 0.05). CONCLUSION: Reduction of TNF-alpha and p38MAPK and increased expression of occludin and ZO-1 in colonic tissue rep
基金supported by State Key Program of National Natural Science Foundation of China (Grant No. 40830957)Public Welfare Research Project of China (Grant No. GYHY200806021)
文摘The observation stations of Northern China are divided into three regions:the arid Northwest China,the Loess Plateau,and the cool Northeast China.The consistencies,differences,and associated mechanisms of land surface thermal-hydrologic processes among the three regions were studied based on the normalization of major variables of land surface thermal-hydrologic processes,using data collected during prevailing summer monsoon period(July and August,2008).It is shown that differences of surface thermal-hydrologic processes are remarkable among the three regions because of different impacts of summer monsoon.Especially their soil wet layers occur at different depths,and the average albedo and its diurnal variations are distinctly different.Surface net short-wave radiation in the Loess Plateau is close to that in the cool Northeast China,but its surface net long-wave radiation is close to that in the arid Northwest China.And the ratio of net radiation to global solar radiation in the cool Northeast China is higher than the other two regions,though its temperature is lower.There are obvious regional differences in the ratios of surface sensible and latent heat fluxes to net radiation for the three regions because of distinct contribution of sensible and latent heat fluxesto land surface energy balance.The three regions are markedly different in the ratio of water vapor flux to pan evaporation,but they are consistent in the ratio of water vapor flux to precipitation.These results not only indicate different influences of climate and environmental factors on land surface thermal-hydrologic processes in the three regions,but also show that summer monsoon is important in the formation and variation of the pattern of land surface thermal-hydrologic processes.
