[Objectives] To study the acute toxicity,mutation,and feeding experiment of polysaccharides from Dicliptera chinensis( L.)Juss.,and assess its safety. [Methods]In accordance with Procedures for toxicological Assessmen...[Objectives] To study the acute toxicity,mutation,and feeding experiment of polysaccharides from Dicliptera chinensis( L.)Juss.,and assess its safety. [Methods]In accordance with Procedures for toxicological Assessment on Food Safety,acute toxicity to mice,micronucleus experiment for bone marrow cell in mice,sperm shape abnormality test in mice,and Ames experiment were carried out,and 30 d feeding experiment was detected for rats. [Results] when the maximum tolerance dose( MTD) of mice was higher than 20 g/kg,at polysaccharides from Dicliptera chinensis( L.) Juss. ≤10 g/kg,there was no statistical difference between the control group and mouse bone marrow cell micronucleus and sperm shape abnormality test group; Ames experiment indicated that the number of revertant colonies of different dose groups of polysaccharides from Dicliptera chinensis( L.) Juss. with and without S9 did not exceed two times of the blank control group; rat 30 d feeding experiment indicated that when polysaccharides from Dicliptera chinensis( L.) Juss. ≤ 3. 2 g/kg,rats grew and developed well,and there was no statistical difference with the control group in blood,multiple organ biochemical indicators and liver tissue. [Conclusions]Polysaccharides from Dicliptera chinensis( L.) Juss. are safe and non-toxic chemical compounds and can be used as health products or drugs.展开更多
基金Supported by Project of National Natural Science Foundation(81360685)Traditional Chinese Medicine Science and Technology Planning Project of Health and Family Planning Commission of Guangxi Zhuang Autonomous Region(GZBZ16-15)
文摘[Objectives] To study the acute toxicity,mutation,and feeding experiment of polysaccharides from Dicliptera chinensis( L.)Juss.,and assess its safety. [Methods]In accordance with Procedures for toxicological Assessment on Food Safety,acute toxicity to mice,micronucleus experiment for bone marrow cell in mice,sperm shape abnormality test in mice,and Ames experiment were carried out,and 30 d feeding experiment was detected for rats. [Results] when the maximum tolerance dose( MTD) of mice was higher than 20 g/kg,at polysaccharides from Dicliptera chinensis( L.) Juss. ≤10 g/kg,there was no statistical difference between the control group and mouse bone marrow cell micronucleus and sperm shape abnormality test group; Ames experiment indicated that the number of revertant colonies of different dose groups of polysaccharides from Dicliptera chinensis( L.) Juss. with and without S9 did not exceed two times of the blank control group; rat 30 d feeding experiment indicated that when polysaccharides from Dicliptera chinensis( L.) Juss. ≤ 3. 2 g/kg,rats grew and developed well,and there was no statistical difference with the control group in blood,multiple organ biochemical indicators and liver tissue. [Conclusions]Polysaccharides from Dicliptera chinensis( L.) Juss. are safe and non-toxic chemical compounds and can be used as health products or drugs.
