Background: The emergence and spread of multidrug-resistant bacteria have become a major public health problem worldwide, particularly in developing countries such as Burkina Faso. This study aims to determine phenoty...Background: The emergence and spread of multidrug-resistant bacteria have become a major public health problem worldwide, particularly in developing countries such as Burkina Faso. This study aims to determine phenotypic and genotypic antibiotic resistant diarrheagenic Escherichia coli (DEC) from patients with diarrhea in Ouagadougou, Burkina Faso. Methodology: Microbiological and biochemical analysis were done to detect two hundred and ninety-two (292) strains. The susceptibility of the strains to antibiotics was determined by the agar disc diffusion method. 16-plex-PCR assays were carried out to detect both virulence and resistance genes encoding betalactams, quinolones, phenicols, tetracyclines and virulence gene of DEC. Results: Diarrheagenic Escherichia coli was detected in 8% (23/292) of patients with diarrhea using the 16-plex-PCR and 39.1% (9/23) of the DEC detected carry at least one resistance gene. Resistance rate in disc diffusion test was 86.96% to tetracycline, 65.23% to cotrimoxazole, 17.4% to nalidixic acid, 17.4% to norfloxacin, 17.4% to ciprofloxacin, 13.04% to ceftriaxone, 13.04% to cefotaxime, 8.7% to gentamicin, 8.7% to Chloramphenicol, 0% to netilmicin. The prevalence of different resistance genes in the studied strains varied from 44.4% to 5.5%. The gene Tet coding for resistance to tetracycline was found in 8 strains (44.4%). The CatA gene coding for resistance to Chloramphenicol was detected in 38.9% of isolates. The qnrS, bla<sub>SHV</sub> and bla<sub>OXA</sub> genes were each detected in 5.5% of isolates. No strain hosts the qnrA, qnrB and bla<sub>TEM</sub> genes. Conclusion: This study identified β-lactams, quinolones, phenicols and tetracyclines resistance genes in DEC isolates from patients with diarrhea in Ouagadougou, Burkina Faso. These results indicate the need for a surveillance program to reduce the prevalence of resistance to Enterobacteriaceae strains in hospitals.展开更多
<b><span style="font-family:Verdana;">Background and Purpose: </span></b><span style="font-family:;" "=""><span style="font-family:Verdana;"...<b><span style="font-family:Verdana;">Background and Purpose: </span></b><span style="font-family:;" "=""><span style="font-family:Verdana;">Diarrhoeagenic </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (DEC) is one of the germs responsible for childhood diarrhea in developing countries. This study aims at determining the prevalence of the five main pathotypes of DEC isolated from faeces of children under five years old with diarrhea or not, living in the city of Koula-Moutou. </span><b><span style="font-family:Verdana;">Methodology: </span></b><span style="font-family:Verdana;">Isolates of </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> were phenotypically screened on chromID</span><sup><span style="font-family:Verdana;">TM</span></sup><span style="font-family:Verdana;"> agar and molecularly by multiplex PCR to detect the </span><span><span style="font-family:Verdana;">presence of enteroaggregative </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (EAEC), enteropathogenic </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (EPEC), </span></span><span style="font-family:Verdana;">enterotoxigenic </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (ETEC), enterohemorragic </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (EHEC) and enteroinvasive </span><i><span style="font-family:Verdana;">E. coli </span></i><span style="font-family:Verdana;">(EIEC). The evaluation of their sensitivity to 12 </span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-lactam antibiotic molecules was carried out by Kirby Bauer method. This method has also made it possible to characterize phenotypically the different </span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-展开更多
Diarrhea,as a global public health problem,causes a large number of infections and deaths every year.Although Escherichia coli(E.coli)is one of the normal flo ra microorganisms in the human intestinal tract,it has fiv...Diarrhea,as a global public health problem,causes a large number of infections and deaths every year.Although Escherichia coli(E.coli)is one of the normal flo ra microorganisms in the human intestinal tract,it has five pathogenic bacteria types that can cause human diarrhea,known as diarrheagenic E.coli.When people are infected,rapid and accurate diagnosis,along with timely treatment,are especially important.Here,we introduce a new method to identify and analyze a large number of pathogenic strains in E.coli by multiplex PCR and barcoded magnetic bead hybridization.Results show that the detection sensitivities of enterohemorrhagic E.coli,enterotoxigenic E.coli,enteropathogenic E.coli,enteroinvasive E.coli and enteroaggregative E.coli were 1.3×10^3 CFU/mL,2×10^4 CFU/mL,4×10^4 CFU/mL,7.2×10^4 CFU/mL and 1.7 CFU/mL respectively.This method has strong specificity and high sensitivity and detects multiple target sequences in one experiment.Compared with other methods,BMB array has great application potential.展开更多
目的了解贵阳地区夏秋季感染性腹泻病例中致泻性大肠杆菌的分布状况,为该地区致泻性大肠杆菌的预防控制提供依据。方法收集2010年贵阳地区6家哨点医院的381例感染性腹泻病例的粪便标本,提取标本DNA,采用实时荧光PCR(RT-PCR)方法进行肠...目的了解贵阳地区夏秋季感染性腹泻病例中致泻性大肠杆菌的分布状况,为该地区致泻性大肠杆菌的预防控制提供依据。方法收集2010年贵阳地区6家哨点医院的381例感染性腹泻病例的粪便标本,提取标本DNA,采用实时荧光PCR(RT-PCR)方法进行肠致病性大肠杆菌(Enteropathogenic E Coli,EPEC)、肠产毒性大肠杆菌(Enterotoxigenic E coli,ETEC)、肠粘附性大肠杆菌(Enteroadherent E coli,EAEC)、肠侵袭性大肠杆菌(Enteroinvasive E coli,EIEC)、肠出血性大肠杆菌(Enterohemorrhagic E coli,EHEC)的检测,并进行病原统计分析。结果 381例腹泻病例的粪便标本中致泻性大肠杆菌检出率为34.65%,其中检出率最高的是EAEC,为12.07%;其次是EPEC,检出率为9.45%,其中非典型EPEC占75.00%;ETEC检出率为9.19%,其中ETEC-ST占60.00%;EIEC检出率为3.94%;EHEC未检出;两种病原混合感染检出率为5.25%,以EPEC和EAEC混合感染为主,占40.00%。≤5岁组和>5岁组病例病原检出率分别为35.28%和30.91%,差异无统计学意义(P>0.05);≤5岁组病原EAEC检出率较高(P<0.05);>5岁组病原ETEC检出率较高(P<0.05)。男性病例检出率高于女性(P<0.05)。8月、9月检出率相对较高(P<0.001)。结论贵阳地区腹泻病例致泻性大肠杆菌感染以EAEC为主,其次是EPEC和ETEC,未发现有EHEC,8月、9月贵阳地区致泻性大肠杆菌的检出率较高。展开更多
文摘Background: The emergence and spread of multidrug-resistant bacteria have become a major public health problem worldwide, particularly in developing countries such as Burkina Faso. This study aims to determine phenotypic and genotypic antibiotic resistant diarrheagenic Escherichia coli (DEC) from patients with diarrhea in Ouagadougou, Burkina Faso. Methodology: Microbiological and biochemical analysis were done to detect two hundred and ninety-two (292) strains. The susceptibility of the strains to antibiotics was determined by the agar disc diffusion method. 16-plex-PCR assays were carried out to detect both virulence and resistance genes encoding betalactams, quinolones, phenicols, tetracyclines and virulence gene of DEC. Results: Diarrheagenic Escherichia coli was detected in 8% (23/292) of patients with diarrhea using the 16-plex-PCR and 39.1% (9/23) of the DEC detected carry at least one resistance gene. Resistance rate in disc diffusion test was 86.96% to tetracycline, 65.23% to cotrimoxazole, 17.4% to nalidixic acid, 17.4% to norfloxacin, 17.4% to ciprofloxacin, 13.04% to ceftriaxone, 13.04% to cefotaxime, 8.7% to gentamicin, 8.7% to Chloramphenicol, 0% to netilmicin. The prevalence of different resistance genes in the studied strains varied from 44.4% to 5.5%. The gene Tet coding for resistance to tetracycline was found in 8 strains (44.4%). The CatA gene coding for resistance to Chloramphenicol was detected in 38.9% of isolates. The qnrS, bla<sub>SHV</sub> and bla<sub>OXA</sub> genes were each detected in 5.5% of isolates. No strain hosts the qnrA, qnrB and bla<sub>TEM</sub> genes. Conclusion: This study identified β-lactams, quinolones, phenicols and tetracyclines resistance genes in DEC isolates from patients with diarrhea in Ouagadougou, Burkina Faso. These results indicate the need for a surveillance program to reduce the prevalence of resistance to Enterobacteriaceae strains in hospitals.
文摘<b><span style="font-family:Verdana;">Background and Purpose: </span></b><span style="font-family:;" "=""><span style="font-family:Verdana;">Diarrhoeagenic </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (DEC) is one of the germs responsible for childhood diarrhea in developing countries. This study aims at determining the prevalence of the five main pathotypes of DEC isolated from faeces of children under five years old with diarrhea or not, living in the city of Koula-Moutou. </span><b><span style="font-family:Verdana;">Methodology: </span></b><span style="font-family:Verdana;">Isolates of </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> were phenotypically screened on chromID</span><sup><span style="font-family:Verdana;">TM</span></sup><span style="font-family:Verdana;"> agar and molecularly by multiplex PCR to detect the </span><span><span style="font-family:Verdana;">presence of enteroaggregative </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (EAEC), enteropathogenic </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (EPEC), </span></span><span style="font-family:Verdana;">enterotoxigenic </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (ETEC), enterohemorragic </span><i><span style="font-family:Verdana;">E. coli</span></i><span style="font-family:Verdana;"> (EHEC) and enteroinvasive </span><i><span style="font-family:Verdana;">E. coli </span></i><span style="font-family:Verdana;">(EIEC). The evaluation of their sensitivity to 12 </span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-lactam antibiotic molecules was carried out by Kirby Bauer method. This method has also made it possible to characterize phenotypically the different </span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-
基金the National Natural Science Foundation of China(Nos.61971187,61571187,61871180)Education Department Outstanding Young Project of Hunan Province(No.18B299)。
文摘Diarrhea,as a global public health problem,causes a large number of infections and deaths every year.Although Escherichia coli(E.coli)is one of the normal flo ra microorganisms in the human intestinal tract,it has five pathogenic bacteria types that can cause human diarrhea,known as diarrheagenic E.coli.When people are infected,rapid and accurate diagnosis,along with timely treatment,are especially important.Here,we introduce a new method to identify and analyze a large number of pathogenic strains in E.coli by multiplex PCR and barcoded magnetic bead hybridization.Results show that the detection sensitivities of enterohemorrhagic E.coli,enterotoxigenic E.coli,enteropathogenic E.coli,enteroinvasive E.coli and enteroaggregative E.coli were 1.3×10^3 CFU/mL,2×10^4 CFU/mL,4×10^4 CFU/mL,7.2×10^4 CFU/mL and 1.7 CFU/mL respectively.This method has strong specificity and high sensitivity and detects multiple target sequences in one experiment.Compared with other methods,BMB array has great application potential.
文摘目的了解贵阳地区夏秋季感染性腹泻病例中致泻性大肠杆菌的分布状况,为该地区致泻性大肠杆菌的预防控制提供依据。方法收集2010年贵阳地区6家哨点医院的381例感染性腹泻病例的粪便标本,提取标本DNA,采用实时荧光PCR(RT-PCR)方法进行肠致病性大肠杆菌(Enteropathogenic E Coli,EPEC)、肠产毒性大肠杆菌(Enterotoxigenic E coli,ETEC)、肠粘附性大肠杆菌(Enteroadherent E coli,EAEC)、肠侵袭性大肠杆菌(Enteroinvasive E coli,EIEC)、肠出血性大肠杆菌(Enterohemorrhagic E coli,EHEC)的检测,并进行病原统计分析。结果 381例腹泻病例的粪便标本中致泻性大肠杆菌检出率为34.65%,其中检出率最高的是EAEC,为12.07%;其次是EPEC,检出率为9.45%,其中非典型EPEC占75.00%;ETEC检出率为9.19%,其中ETEC-ST占60.00%;EIEC检出率为3.94%;EHEC未检出;两种病原混合感染检出率为5.25%,以EPEC和EAEC混合感染为主,占40.00%。≤5岁组和>5岁组病例病原检出率分别为35.28%和30.91%,差异无统计学意义(P>0.05);≤5岁组病原EAEC检出率较高(P<0.05);>5岁组病原ETEC检出率较高(P<0.05)。男性病例检出率高于女性(P<0.05)。8月、9月检出率相对较高(P<0.001)。结论贵阳地区腹泻病例致泻性大肠杆菌感染以EAEC为主,其次是EPEC和ETEC,未发现有EHEC,8月、9月贵阳地区致泻性大肠杆菌的检出率较高。
