Antibiotics residues have been accumulating in the environment day by day due to overuse of antibiotics.Recalcitrant antibiotic residues,such as tylosin(TYL),can cause serious environmental problems,which makes it imp...Antibiotics residues have been accumulating in the environment day by day due to overuse of antibiotics.Recalcitrant antibiotic residues,such as tylosin(TYL),can cause serious environmental problems,which makes it important to eliminate TYL from the environment.It is important to eliminate TYL from the environment.In this study,a strain was isolated and purified from fermentation by-product that came from a TYL production factory.The TYL degrading strain was identified by its morphology,physiological and biochemical reactions and sequencing the PCR-amplified fragments of its 16 S r DNAcoding genes.The temperature,shaking speed,initial TYL concentration,p H and inoculum sizes were investigated under simulated conditions by using single factor tests.The results showed that TYL2,a high efficient strain was isolated and was identified as Brevibacillus borstelensis.The degradation rate of TYL by this strain could reach to 75%with an initial concentration of 25 mg L^-1 within 7 days under conditions of 7%B.borstelensis(v/v,2×108 CFU m L^-1)at p H 7.0 and at 35°C.It is interesting that this strain has a very strong ability to degrade the TYL in natural sewage with the degradation rate of 65%within 7 days.This result could be helpful for the degradation of TYL and provide guidance for the degradation of other antibiotics.展开更多
【目的】解决大蒜废水难以生化处理的问题,筛选能够降解大蒜素的细菌,并对其生化特性进行分析。【方法】通过16S r RNA基因测序,对大蒜废水沉积物进行细菌多样性分析,发现其中有多种大蒜素耐受菌;通过富集培养与划线分离大蒜素降解菌,...【目的】解决大蒜废水难以生化处理的问题,筛选能够降解大蒜素的细菌,并对其生化特性进行分析。【方法】通过16S r RNA基因测序,对大蒜废水沉积物进行细菌多样性分析,发现其中有多种大蒜素耐受菌;通过富集培养与划线分离大蒜素降解菌,并进一步分析其最适生长和大蒜素降解条件。【结果】得到一株大蒜素降解菌(JX6-2),能够以大蒜素为唯一碳源生长(50-300 mg/L),将大蒜素彻底降解。其最适生长温度和降解大蒜素温度均为35°C,最适pH值在中性左右,添加葡萄糖对大蒜素降解没有明显影响。【结论】分离得到了一株大蒜素降解菌,分析了其生长和降解特性,为生化方法处理大蒜素废水提供了新菌种。展开更多
基金jointly supported by the National Key R&D Program of China(2018YFD0500206)the National Natural Science Foundation of China(31772395)the Fundamental Research Funds for Central Non-profit Scientific Institution,Chinese Academy of Agricultural Sciences(1610132019046)。
文摘Antibiotics residues have been accumulating in the environment day by day due to overuse of antibiotics.Recalcitrant antibiotic residues,such as tylosin(TYL),can cause serious environmental problems,which makes it important to eliminate TYL from the environment.It is important to eliminate TYL from the environment.In this study,a strain was isolated and purified from fermentation by-product that came from a TYL production factory.The TYL degrading strain was identified by its morphology,physiological and biochemical reactions and sequencing the PCR-amplified fragments of its 16 S r DNAcoding genes.The temperature,shaking speed,initial TYL concentration,p H and inoculum sizes were investigated under simulated conditions by using single factor tests.The results showed that TYL2,a high efficient strain was isolated and was identified as Brevibacillus borstelensis.The degradation rate of TYL by this strain could reach to 75%with an initial concentration of 25 mg L^-1 within 7 days under conditions of 7%B.borstelensis(v/v,2×108 CFU m L^-1)at p H 7.0 and at 35°C.It is interesting that this strain has a very strong ability to degrade the TYL in natural sewage with the degradation rate of 65%within 7 days.This result could be helpful for the degradation of TYL and provide guidance for the degradation of other antibiotics.
文摘【目的】解决大蒜废水难以生化处理的问题,筛选能够降解大蒜素的细菌,并对其生化特性进行分析。【方法】通过16S r RNA基因测序,对大蒜废水沉积物进行细菌多样性分析,发现其中有多种大蒜素耐受菌;通过富集培养与划线分离大蒜素降解菌,并进一步分析其最适生长和大蒜素降解条件。【结果】得到一株大蒜素降解菌(JX6-2),能够以大蒜素为唯一碳源生长(50-300 mg/L),将大蒜素彻底降解。其最适生长温度和降解大蒜素温度均为35°C,最适pH值在中性左右,添加葡萄糖对大蒜素降解没有明显影响。【结论】分离得到了一株大蒜素降解菌,分析了其生长和降解特性,为生化方法处理大蒜素废水提供了新菌种。
