目的从基因水平探讨湖北地区汉族人食管癌 HEN-DQB1等位基因的遗传易感性.方法运用序列特异性引物聚合酶链反应技术,检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的 HLA-DQB1等位基因.SAS system 统计软件数据处理.结果湖北汉...目的从基因水平探讨湖北地区汉族人食管癌 HEN-DQB1等位基因的遗传易感性.方法运用序列特异性引物聚合酶链反应技术,检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的 HLA-DQB1等位基因.SAS system 统计软件数据处理.结果湖北汉族人食管癌患者与正常人比较,HEN-DQB1*0301基因频率显著增高(0.2976 vs 0.1875),P=0.046,OR=1.835,病因分数=0.1354);两组间 HLA-DQB1其余各等位基因分布频率的比较,HLA-DQB1*0201(0.0833 vs 0.1016),*0301(0.2976 vs 0.1875),*0302(0.0595 vs 0859),*0303(0.2381 vs 0.1875),*0304(0.0000 vs 0.0039),*0401(0.0714 vs 0.0469),*0402(0.0119 vs 0.0156),*0501(0.0357 vs 0.0703),*0502(0.0595 vs 0.0664),*0503(0.0119 vs 0.0195),*0504(0.0000 vs 0.0039),*0601(0.0595 vs 0.0781),*0602(0.0476 vs 0.0742),*0603(0.0000 vs 0.0078),*0604(0.0238 vs 0.0508),差异均无显著性.结论 HLA-DQB1*0301等位基因与湖北汉族人食管癌正关联,为其易感基因.展开更多
The HLA genes, located on the short arm of human chromosome 6, encode peptides involved in host immune response, are important in tissue transplantation and are associated with a variety of infectious, autoimmune, and...The HLA genes, located on the short arm of human chromosome 6, encode peptides involved in host immune response, are important in tissue transplantation and are associated with a variety of infectious, autoimmune, and inflammatory diseases. Moreover, the HLA loci display an unprecedented degree of diversity and the distribution of HLA alleles and haplotypes among different populations is considerably variable. The expression of particular HLA alleles may be associated with the susceptibility or resistance to some diseases. In this study, the genetic polymorphism of HLA-DQA1 and -DQB1 in Jiangsu Han population was analyzed by polymerase chain reaction-sequence-based typing （PCR-SBT）.展开更多
为探究金堂黑山羊主要组织相容性复合体II类基因(MHC class II DQ-beta 1 chain gene,DQB1)的结构与功能,及其组织表达特征。本研究采用PCR扩增DQB1基因并对其进行生物信息学分析,同时采用荧光定量PCR技术检测其在金堂黑山羊不同组织中...为探究金堂黑山羊主要组织相容性复合体II类基因(MHC class II DQ-beta 1 chain gene,DQB1)的结构与功能,及其组织表达特征。本研究采用PCR扩增DQB1基因并对其进行生物信息学分析,同时采用荧光定量PCR技术检测其在金堂黑山羊不同组织中的转录情况。结果显示:金堂黑山羊DQB1基因长度为786 bp,编码261个氨基酸。同源性分析显示金堂黑山羊DQB1基因与8个物种的相应基因同源性均达到85%以上,表明DQB1基因具有较高的保守性。荧光定量PCR结果显示,该基因在金堂黑山羊心脏组织中转录水平显著高于其在脾、肌肉、肠的转录水平(p<0.01),表明DQB1基因在不同组织中的转录水平存在一定差异。本研究为进一步探究DQB1的免疫功能提供参考依据。展开更多
文摘目的从基因水平探讨湖北地区汉族人食管癌 HEN-DQB1等位基因的遗传易感性.方法运用序列特异性引物聚合酶链反应技术,检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的 HLA-DQB1等位基因.SAS system 统计软件数据处理.结果湖北汉族人食管癌患者与正常人比较,HEN-DQB1*0301基因频率显著增高(0.2976 vs 0.1875),P=0.046,OR=1.835,病因分数=0.1354);两组间 HLA-DQB1其余各等位基因分布频率的比较,HLA-DQB1*0201(0.0833 vs 0.1016),*0301(0.2976 vs 0.1875),*0302(0.0595 vs 0859),*0303(0.2381 vs 0.1875),*0304(0.0000 vs 0.0039),*0401(0.0714 vs 0.0469),*0402(0.0119 vs 0.0156),*0501(0.0357 vs 0.0703),*0502(0.0595 vs 0.0664),*0503(0.0119 vs 0.0195),*0504(0.0000 vs 0.0039),*0601(0.0595 vs 0.0781),*0602(0.0476 vs 0.0742),*0603(0.0000 vs 0.0078),*0604(0.0238 vs 0.0508),差异均无显著性.结论 HLA-DQB1*0301等位基因与湖北汉族人食管癌正关联,为其易感基因.
基金This study was supported by a grant from the National Natural Science Foundation of China (No. 30200232).
文摘The HLA genes, located on the short arm of human chromosome 6, encode peptides involved in host immune response, are important in tissue transplantation and are associated with a variety of infectious, autoimmune, and inflammatory diseases. Moreover, the HLA loci display an unprecedented degree of diversity and the distribution of HLA alleles and haplotypes among different populations is considerably variable. The expression of particular HLA alleles may be associated with the susceptibility or resistance to some diseases. In this study, the genetic polymorphism of HLA-DQA1 and -DQB1 in Jiangsu Han population was analyzed by polymerase chain reaction-sequence-based typing （PCR-SBT）.
文摘为探究金堂黑山羊主要组织相容性复合体II类基因(MHC class II DQ-beta 1 chain gene,DQB1)的结构与功能,及其组织表达特征。本研究采用PCR扩增DQB1基因并对其进行生物信息学分析,同时采用荧光定量PCR技术检测其在金堂黑山羊不同组织中的转录情况。结果显示:金堂黑山羊DQB1基因长度为786 bp,编码261个氨基酸。同源性分析显示金堂黑山羊DQB1基因与8个物种的相应基因同源性均达到85%以上,表明DQB1基因具有较高的保守性。荧光定量PCR结果显示,该基因在金堂黑山羊心脏组织中转录水平显著高于其在脾、肌肉、肠的转录水平(p<0.01),表明DQB1基因在不同组织中的转录水平存在一定差异。本研究为进一步探究DQB1的免疫功能提供参考依据。
