Lithium–sulfur batteries with an ultrahigh theoretical energy density of 2600 Wh kg^(−1) are highly consid-ered as desirable next-generation energy storage devices that will meet the growing demand of energy consumpt...Lithium–sulfur batteries with an ultrahigh theoretical energy density of 2600 Wh kg^(−1) are highly consid-ered as desirable next-generation energy storage devices that will meet the growing demand of energy consumption worldwide.However,complicated sul-fur redox reactions and polysulfide shuttling signifi-cantly postpone the applications of lithium-sulfur batteries with rapid capacity decay and low Coulom-bic efficiency.展开更多
To fabricate a highly biocompatible nanoplatform enabling synergistic therapy and real-time imaging,novel Au@Bi2S3 core shell nanobones(NBs)(Au@Bi2S3 NBs)with Au nanorods as cores were synthesized.The combination of A...To fabricate a highly biocompatible nanoplatform enabling synergistic therapy and real-time imaging,novel Au@Bi2S3 core shell nanobones(NBs)(Au@Bi2S3 NBs)with Au nanorods as cores were synthesized.The combination of Au nanorods with Bi2S3 film made the Au@Bi2S3 NBs exhibit ultrahigh photothermal(PT)conversion efficiency,remarkable photoacoustic(PA)imaging and high computed tomography(CT)performance;these Au@Bi2S3 NBs thus are a promising nanotheranostic agent for PT/PA/CT imaging.Subsequently,poly(N-vinylpyrrolidone)-modified Au@Bi2S3 NBs(Au@Bi2S3-PVP NBs)were successfully loaded with the anticancer drug doxorubicin(DOX),and a satisfactory pH sensitive release profile was achieved,thus revealing the great potential of Au@Bi2S3-PVP NBs in chemotherapy as a drug carrier to deliver DOX into cancer cells.Both in vitro and in vivo investigations demonstrated that the Au@Bi2S3-PVP NBs possessed multiple desired features for cancer therapy,including extremely low toxicity,good biocompatibility,high drug loading ability,precise tumor targeting and effective accumulation.Highly efficient ablation of the human liver cancer cell HepG2 was achieved through Au@Bi2S3-PVP NB-mediated photothermal therapy(PTT).As both a contrast enhancement probe and therapeutic agent,Au@Bi2S3-PVP NBs provided outstanding NIR-triggered multi-modal PT/PA/CT imaging-guided PTT and effectively inhibited the growth of HepG2 liver cancer cells via synergistic chemo/PT therapy.展开更多
Resistance to breast cancer(BCa) chemotherapy severely hampers the patient’s prognosis.MicroRNAs provide a potential therapeutic prospect for BCa.In this study,the reversal function of microRNA34 a(miR34 a) on doxoru...Resistance to breast cancer(BCa) chemotherapy severely hampers the patient’s prognosis.MicroRNAs provide a potential therapeutic prospect for BCa.In this study,the reversal function of microRNA34 a(miR34 a) on doxorubicin(Dox) resistance of BCa and the possible mechanism was investigated.We found that the relative level of miR34 a was significantly decreased in Dox-resistant breast cancer cell MCF-7(MCF-7/A) compared with Dox-sensitive MCF-7 cells.Transfection with miR34 a significantly suppressed the invasion,migration,adhesion of MCF-7/A cells without inhibiting their growth obviously.The combination of miR34 a and Dox could significantly inhibit the proliferation,migration,invasion and induce the apoptosis of MCF-7/A cells.The synergistic effect of this combination on resistant MCF-7/A cells has no obvious relation with the expressions of classical drug-resistant proteins P-GP,MRP and GST-π,while closely related with the down-regulation on TOP2 A and BCRP.Moreover,we found both protein and mRNA expression of Snail were significantly up-regulated in MCF-7/A cells in comparison with MCF-7 cells.Transfection with small interfering RNA(siRNA) of Snail could inhibit the invasion,migration and adhesion of drug-resistant MCF-7/A cells,while highexpression of Snail could remarkably promote the invasion,migration and adhesion of MCF-7 cells,which might be related with regulation of N-cadherin and E-cadherin.Transfection with miR34 a in MCF-7/A cells induced a decrease of Snail expression.The potential binding sites of miR34 a with 3’UTR of Snail were predicted by miRDB target prediction software,which was confirmed by luciferase reporter gene method.Results showed that the relative activity of luciferase was reduced in MCF-7/A cells after co-transfection of miR34 a and wild type(wt)-Snail,while did not change by co-transfection with miR34 a and 3’ UTR mutant type(mut) Snail.Combination of miR34 a and Dox induced a stronger decrease of Snail in MCF-7/A cells in comparison to miR34 a or Dox treatment alone.What’展开更多
Chemotherapy,the use of antitumor drugs to kill cancer cells,is currently one of the most effective treatments for cancer.However,serious toxic side effects caused by long-term drug accumulation can cause significant ...Chemotherapy,the use of antitumor drugs to kill cancer cells,is currently one of the most effective treatments for cancer.However,serious toxic side effects caused by long-term drug accumulation can cause significant damage to the body,which limits the clinical application of antitumor drugs.In this study,a novel RENPs@DOX-Fe nanoprobe(NP) was constructed by coating the surface of rare earth nanomaterials(NaLuF_(4):Yb,Er) with a complex formed by doxorubicin(DOX) and iron ion(Ⅲ).Due to the low toxicity of anthracycline-metal complexes,the damage to normal cells is reduced.The unique acidic microenvironment in tumor cells facilitates the decomposition and gradual release of DOX from the DOX-Fe complex.In addition,the DOX-Fe complex can convert near-infrared(NIR) light into heat energy,which promotes the decomposition of the complex,further enhancing the release of DOX in the tumor environment.The change of ratio fluorescence of rare earth nanomaterials at 660 and 1550 nm after DOX release enables visual monitoring of drug release,which can potentially improve the chemotherapeutic effect.In vitro experiments established that RENPs@DOX-Fe NPs with NIR illumination had good therapeutic efficacy in tumors.This work provides new insights into designing tumor microenvironment-responsive nanoprobes for chemotherapy with minimal side effects.展开更多
DNAzyme-based gene therapy faces some challenges including cell penetration,activity limitation,and co-delivery functions.Self-assembled DNA nanomedicine has attracted widespread attention due to its many advantages.I...DNAzyme-based gene therapy faces some challenges including cell penetration,activity limitation,and co-delivery functions.Self-assembled DNA nanomedicine has attracted widespread attention due to its many advantages.It is urgent to develop a universal DNA degradation strategy for precise programmable drug release.Herein,we reported a self-catabolic DNAzyme nanospheres(SCNS),which could simultaneously achieve cell penetration,activity enhancement,and co-delivery functions.The SCNS were assembled through Y-DNA stepwise hybridization with each other,which were then loaded with aptamer(Apt),doxorubicin(Dox),and zinc oxide nanoparticles(ZnO NPs).The acid-triggered dissociation of ZnO NPs leads to the generation of Zn^(2+)ions cofactors for immediately self-catabolic DNAzyme nanospheres.After the disassembly of the SCNS,three types of anticancer treatments would be activated,which include Zn^(2+)involved reactive oxygen species(ROS),Dox-induced chemotherapy,and DNAzyme-based gene therapy.The experimental results show that the nanoplatform(Apt-SCNS-Dox-ZnO)has a good tumor-killing effect and minimal side effects.As a smart self-driven drug delivery nanoplatform,it is anticipated to displace extraordinary potential in biomedicine and bioengineering.展开更多
基金This work was supported by National Key Re-search and Development Program(2016YFA0202500,2015CB932500,and2016YFA0200102)National Natural Scientific Foundation of China(21676160 and 21825501)Tsinghua University Initiative Scientific Research Program.
文摘Lithium–sulfur batteries with an ultrahigh theoretical energy density of 2600 Wh kg^(−1) are highly consid-ered as desirable next-generation energy storage devices that will meet the growing demand of energy consumption worldwide.However,complicated sul-fur redox reactions and polysulfide shuttling signifi-cantly postpone the applications of lithium-sulfur batteries with rapid capacity decay and low Coulom-bic efficiency.
基金This work was financially supported by the Natural Science Foundation of Shanghai(19ZR1434800,19ZR1461900)the National Natural Science Foundation of China(21305090)+1 种基金the Fundamental Research Funds for the Central Universities(to Shuang Zhou)The authors greatly appreciated these supports.
文摘To fabricate a highly biocompatible nanoplatform enabling synergistic therapy and real-time imaging,novel Au@Bi2S3 core shell nanobones(NBs)(Au@Bi2S3 NBs)with Au nanorods as cores were synthesized.The combination of Au nanorods with Bi2S3 film made the Au@Bi2S3 NBs exhibit ultrahigh photothermal(PT)conversion efficiency,remarkable photoacoustic(PA)imaging and high computed tomography(CT)performance;these Au@Bi2S3 NBs thus are a promising nanotheranostic agent for PT/PA/CT imaging.Subsequently,poly(N-vinylpyrrolidone)-modified Au@Bi2S3 NBs(Au@Bi2S3-PVP NBs)were successfully loaded with the anticancer drug doxorubicin(DOX),and a satisfactory pH sensitive release profile was achieved,thus revealing the great potential of Au@Bi2S3-PVP NBs in chemotherapy as a drug carrier to deliver DOX into cancer cells.Both in vitro and in vivo investigations demonstrated that the Au@Bi2S3-PVP NBs possessed multiple desired features for cancer therapy,including extremely low toxicity,good biocompatibility,high drug loading ability,precise tumor targeting and effective accumulation.Highly efficient ablation of the human liver cancer cell HepG2 was achieved through Au@Bi2S3-PVP NB-mediated photothermal therapy(PTT).As both a contrast enhancement probe and therapeutic agent,Au@Bi2S3-PVP NBs provided outstanding NIR-triggered multi-modal PT/PA/CT imaging-guided PTT and effectively inhibited the growth of HepG2 liver cancer cells via synergistic chemo/PT therapy.
基金supported by Natural Science Foundation of Shandong Province(ZR2020MH419,China)Key Research and Development Program of Shandong Province(2017CXGC1401,China)+1 种基金Chinae Australia Centre for Health Sciences Research(CACHSR No.2019GJ01)Major Basic Research Projects of Shandong Province(ZR2018ZC0233,China)
文摘Resistance to breast cancer(BCa) chemotherapy severely hampers the patient’s prognosis.MicroRNAs provide a potential therapeutic prospect for BCa.In this study,the reversal function of microRNA34 a(miR34 a) on doxorubicin(Dox) resistance of BCa and the possible mechanism was investigated.We found that the relative level of miR34 a was significantly decreased in Dox-resistant breast cancer cell MCF-7(MCF-7/A) compared with Dox-sensitive MCF-7 cells.Transfection with miR34 a significantly suppressed the invasion,migration,adhesion of MCF-7/A cells without inhibiting their growth obviously.The combination of miR34 a and Dox could significantly inhibit the proliferation,migration,invasion and induce the apoptosis of MCF-7/A cells.The synergistic effect of this combination on resistant MCF-7/A cells has no obvious relation with the expressions of classical drug-resistant proteins P-GP,MRP and GST-π,while closely related with the down-regulation on TOP2 A and BCRP.Moreover,we found both protein and mRNA expression of Snail were significantly up-regulated in MCF-7/A cells in comparison with MCF-7 cells.Transfection with small interfering RNA(siRNA) of Snail could inhibit the invasion,migration and adhesion of drug-resistant MCF-7/A cells,while highexpression of Snail could remarkably promote the invasion,migration and adhesion of MCF-7 cells,which might be related with regulation of N-cadherin and E-cadherin.Transfection with miR34 a in MCF-7/A cells induced a decrease of Snail expression.The potential binding sites of miR34 a with 3’UTR of Snail were predicted by miRDB target prediction software,which was confirmed by luciferase reporter gene method.Results showed that the relative activity of luciferase was reduced in MCF-7/A cells after co-transfection of miR34 a and wild type(wt)-Snail,while did not change by co-transfection with miR34 a and 3’ UTR mutant type(mut) Snail.Combination of miR34 a and Dox induced a stronger decrease of Snail in MCF-7/A cells in comparison to miR34 a or Dox treatment alone.What’
基金Project supported by the National Natural Science Foundation of China(92159103)Beijing Municipal Education Commission Outstanding Young Individual Project(CIT&TCD201904082)+1 种基金Youth High-level Talent Project of Capital Normal University(20530810024)Yanjing Young Scholar Program of Capital Normal University。
文摘Chemotherapy,the use of antitumor drugs to kill cancer cells,is currently one of the most effective treatments for cancer.However,serious toxic side effects caused by long-term drug accumulation can cause significant damage to the body,which limits the clinical application of antitumor drugs.In this study,a novel RENPs@DOX-Fe nanoprobe(NP) was constructed by coating the surface of rare earth nanomaterials(NaLuF_(4):Yb,Er) with a complex formed by doxorubicin(DOX) and iron ion(Ⅲ).Due to the low toxicity of anthracycline-metal complexes,the damage to normal cells is reduced.The unique acidic microenvironment in tumor cells facilitates the decomposition and gradual release of DOX from the DOX-Fe complex.In addition,the DOX-Fe complex can convert near-infrared(NIR) light into heat energy,which promotes the decomposition of the complex,further enhancing the release of DOX in the tumor environment.The change of ratio fluorescence of rare earth nanomaterials at 660 and 1550 nm after DOX release enables visual monitoring of drug release,which can potentially improve the chemotherapeutic effect.In vitro experiments established that RENPs@DOX-Fe NPs with NIR illumination had good therapeutic efficacy in tumors.This work provides new insights into designing tumor microenvironment-responsive nanoprobes for chemotherapy with minimal side effects.
基金supported by the National Natural Science Foundation of China(22174042 and 22374038)the Natural Science Foundation for Distinguished Young Scholars of Hunan Province(2021JJ10011)the Postgraduate Scientific Research Innovation Project of Hunan Province(CX20220390)。
文摘DNAzyme-based gene therapy faces some challenges including cell penetration,activity limitation,and co-delivery functions.Self-assembled DNA nanomedicine has attracted widespread attention due to its many advantages.It is urgent to develop a universal DNA degradation strategy for precise programmable drug release.Herein,we reported a self-catabolic DNAzyme nanospheres(SCNS),which could simultaneously achieve cell penetration,activity enhancement,and co-delivery functions.The SCNS were assembled through Y-DNA stepwise hybridization with each other,which were then loaded with aptamer(Apt),doxorubicin(Dox),and zinc oxide nanoparticles(ZnO NPs).The acid-triggered dissociation of ZnO NPs leads to the generation of Zn^(2+)ions cofactors for immediately self-catabolic DNAzyme nanospheres.After the disassembly of the SCNS,three types of anticancer treatments would be activated,which include Zn^(2+)involved reactive oxygen species(ROS),Dox-induced chemotherapy,and DNAzyme-based gene therapy.The experimental results show that the nanoplatform(Apt-SCNS-Dox-ZnO)has a good tumor-killing effect and minimal side effects.As a smart self-driven drug delivery nanoplatform,it is anticipated to displace extraordinary potential in biomedicine and bioengineering.
