Purpose: To determine the DNA strand breaks, oxidative DNA damage and cell death in blood and plasma total antioxidant status (TAOS) in 22 patients with severe multiple trauma. Materials and methods: The DNA comet ass...Purpose: To determine the DNA strand breaks, oxidative DNA damage and cell death in blood and plasma total antioxidant status (TAOS) in 22 patients with severe multiple trauma. Materials and methods: The DNA comet assay was used to measure DNA strand breakage, 8-oxoguanine levels and apoptotic and necrotic nuclei in after admission (day 0) and on days 3, 5, 7 and 15. TAOS was determined by colorimetric method. Results: Trauma patients had high DNA damage at admission (p < 0.01), that further increased with maximum value on day 5 (p < 0.001). On day 15 the degree of DNA damage remained significantly elevated (p < 0.01). No significant difference in the 8-oxoguanine levels at all days examined was found. Patients had a high percentage of apoptotic and necrotic comets at admission, with maximum values on days 3 and 5. A significantly lower TAOS was observed in patients on admission and days 3, 5, 7 and 15 (p < 0.001 in all cases). A decreasing of TAOS on days 7 and 15 compared to that on admission (p < 0.05) was observed. Conclusions: Blood cells from severe trauma patients’ display increased DNA damage associated with apoptosis and necrosis. Reduced plasma TAOS and a tendency to increase of 8-oxoguanine in DNA was observed.展开更多
Marine pollution affects all life processes in aquatic organisms. The genotoxic effect of pollution on the mussel Crenomytilus grayanus was assessed. Bivalves were collected from the ‘clean'(Vostochnaya Cove) and...Marine pollution affects all life processes in aquatic organisms. The genotoxic effect of pollution on the mussel Crenomytilus grayanus was assessed. Bivalves were collected from the ‘clean'(Vostochnaya Cove) and polluted(Nakhodka Bay) areas in the Peter the Great Bay. The degree of DNA damage in C. grayanus was determined by alkaline comet assay as mean percentage of DNA in tail, and the genetic damage index was calculated. Our results indicate that almost one-third of DNA in cells of gills and digestive gland of C. grayanus inhabiting the Nakhodka Bay had destructive changes compared to the individuals of this species from the Vostochnaya Cove. This study has shown that chronic pollution of the aquatic environment causes destructive changes to DNA in gill and digestive gland cells of C. grayanus.展开更多
文摘Purpose: To determine the DNA strand breaks, oxidative DNA damage and cell death in blood and plasma total antioxidant status (TAOS) in 22 patients with severe multiple trauma. Materials and methods: The DNA comet assay was used to measure DNA strand breakage, 8-oxoguanine levels and apoptotic and necrotic nuclei in after admission (day 0) and on days 3, 5, 7 and 15. TAOS was determined by colorimetric method. Results: Trauma patients had high DNA damage at admission (p < 0.01), that further increased with maximum value on day 5 (p < 0.001). On day 15 the degree of DNA damage remained significantly elevated (p < 0.01). No significant difference in the 8-oxoguanine levels at all days examined was found. Patients had a high percentage of apoptotic and necrotic comets at admission, with maximum values on days 3 and 5. A significantly lower TAOS was observed in patients on admission and days 3, 5, 7 and 15 (p < 0.001 in all cases). A decreasing of TAOS on days 7 and 15 compared to that on admission (p < 0.05) was observed. Conclusions: Blood cells from severe trauma patients’ display increased DNA damage associated with apoptosis and necrosis. Reduced plasma TAOS and a tendency to increase of 8-oxoguanine in DNA was observed.
文摘Marine pollution affects all life processes in aquatic organisms. The genotoxic effect of pollution on the mussel Crenomytilus grayanus was assessed. Bivalves were collected from the ‘clean'(Vostochnaya Cove) and polluted(Nakhodka Bay) areas in the Peter the Great Bay. The degree of DNA damage in C. grayanus was determined by alkaline comet assay as mean percentage of DNA in tail, and the genetic damage index was calculated. Our results indicate that almost one-third of DNA in cells of gills and digestive gland of C. grayanus inhabiting the Nakhodka Bay had destructive changes compared to the individuals of this species from the Vostochnaya Cove. This study has shown that chronic pollution of the aquatic environment causes destructive changes to DNA in gill and digestive gland cells of C. grayanus.
