Duck enteritis virus(DEV) is a herpesvirus that causes an acute,contagious and fatal disease. In the present article,the DEV UL4 gene was cloned and sequenced from a vaccine virus. A degenerate oligonucleotide primer ...Duck enteritis virus(DEV) is a herpesvirus that causes an acute,contagious and fatal disease. In the present article,the DEV UL4 gene was cloned and sequenced from a vaccine virus. A degenerate oligonucleotide primer for the consensus site of herpesvirus UL3 gene and a specific primer located in UL5 were used in the polymerase chain reaction(PCR) to amplify a DNA product 2 086 bp in size. DNA sequence analysis revealed that a 714 bp open reading frame(ORF) of DEV encoding a 237 amino acid polypeptide is homologous to the family of herpesvirus UL4 proteins and therefore has been characterized as a DEV UL4 gene. Alignment of the DEV UL4 protein sequence with those of other alphaherpesviruses showed that 10 amino acid residues are completely conserved. Phylogenetic tree analysis showed that the seventeen alphaherpesviruses viruses analyzed were classified into four large groups,and the duck enteritis virus branched separately,closely related to the Mardiviruses group comprising Gallid herpesvirus 2(GaHV-2) ,Gallid herpesvirus 3(GaHV-3) and Meleagrid herpesvirus 1(MeHV-1) . The present study showed that the evolutionary relationship of the UL4 protein could be used for classification of alphaherpesviruses.展开更多
This paper describes an integrated platform for machine learning and big data analysis. The integrated platform is configured in a way that builds a large distributed data processing environment in the computing envir...This paper describes an integrated platform for machine learning and big data analysis. The integrated platform is configured in a way that builds a large distributed data processing environment in the computing environment that makes up the NVIDIA AI platform. In addition, this paper describes the background of this idea selection and the use of the software to build the unified platform. The technical details are shown in terms of how to create the proposed platform. In the anlaysis section, the methodology is provided and also the steps are explained as to how to use this integration platform. Finally, the expected effects are elaborated in the conclusion section.展开更多
The debate between domestication and foreignization translation strategies has aroused scholars' research interest for a long time. This thesis makes a comparative study on the application of domestication and for...The debate between domestication and foreignization translation strategies has aroused scholars' research interest for a long time. This thesis makes a comparative study on the application of domestication and foreignization of the Stylistic Devices in Legge's and Zhao Zhentao's translated versions of Mencius.展开更多
文摘Duck enteritis virus(DEV) is a herpesvirus that causes an acute,contagious and fatal disease. In the present article,the DEV UL4 gene was cloned and sequenced from a vaccine virus. A degenerate oligonucleotide primer for the consensus site of herpesvirus UL3 gene and a specific primer located in UL5 were used in the polymerase chain reaction(PCR) to amplify a DNA product 2 086 bp in size. DNA sequence analysis revealed that a 714 bp open reading frame(ORF) of DEV encoding a 237 amino acid polypeptide is homologous to the family of herpesvirus UL4 proteins and therefore has been characterized as a DEV UL4 gene. Alignment of the DEV UL4 protein sequence with those of other alphaherpesviruses showed that 10 amino acid residues are completely conserved. Phylogenetic tree analysis showed that the seventeen alphaherpesviruses viruses analyzed were classified into four large groups,and the duck enteritis virus branched separately,closely related to the Mardiviruses group comprising Gallid herpesvirus 2(GaHV-2) ,Gallid herpesvirus 3(GaHV-3) and Meleagrid herpesvirus 1(MeHV-1) . The present study showed that the evolutionary relationship of the UL4 protein could be used for classification of alphaherpesviruses.
文摘This paper describes an integrated platform for machine learning and big data analysis. The integrated platform is configured in a way that builds a large distributed data processing environment in the computing environment that makes up the NVIDIA AI platform. In addition, this paper describes the background of this idea selection and the use of the software to build the unified platform. The technical details are shown in terms of how to create the proposed platform. In the anlaysis section, the methodology is provided and also the steps are explained as to how to use this integration platform. Finally, the expected effects are elaborated in the conclusion section.
文摘The debate between domestication and foreignization translation strategies has aroused scholars' research interest for a long time. This thesis makes a comparative study on the application of domestication and foreignization of the Stylistic Devices in Legge's and Zhao Zhentao's translated versions of Mencius.
