从EMS诱变日本晴种子的M2代中筛选得到一个植株明显矮化、分蘖数急剧增多的突变体,命名为det1(dwarf and excessively-tillering1)。det1还显示出明显的穗型、粒型和育性等方面的生殖发育缺陷。遗传分析表明,det1受一对隐性基因控制。以...从EMS诱变日本晴种子的M2代中筛选得到一个植株明显矮化、分蘖数急剧增多的突变体,命名为det1(dwarf and excessively-tillering1)。det1还显示出明显的穗型、粒型和育性等方面的生殖发育缺陷。遗传分析表明,det1受一对隐性基因控制。以det1与品种DZ60杂交建立了F2定位群体,利用微卫星标记分析F2群体中的突变体,将DET1基因定位在第6染色体长臂端,标记SSR2和SSR3之间约68kb的范围内。已知该区间内存在一个与水稻株高和分蘖有关的基因D3。测序结果表明,DET1与D3是同一个基因,编码一个LRR型的F-box蛋白。与d3相比,det1还显示了新的表型特征,推测可能是等位基因的不同突变位点造成的。det1/d3的表型特征提示,DET1/D3在调节水稻生长发育过程中具有多效性。因此,利用det1开展进一步的研究将有助于全面揭示DET1/D3的分子功能。展开更多
DE-ETIOLATED 1(DET1)and CONSTITUTIVE PHOTOMORPHOGENESIS 1(COP1)are two essential repressors of Arabidopsis photomorphogenesis.These proteins can associate with CULLIN4 to form independent CRL4-based E3 ubiquitin ligas...DE-ETIOLATED 1(DET1)and CONSTITUTIVE PHOTOMORPHOGENESIS 1(COP1)are two essential repressors of Arabidopsis photomorphogenesis.These proteins can associate with CULLIN4 to form independent CRL4-based E3 ubiquitin ligases that mediate the degradation of several photomorphogenic transcription factors,including ELONGATED HYPOCOTYL 5(HY5),thereby controlling multiple gene-regulatory networks.Despite extensive biochemical and genetic analyses of their multi-subunit complexes,the functional links between DET1 and COP1 have long remained elusive.Here,we report that DET1 associates with COP1 in vivo,enhances COP1-HY5 interaction,and promotes COP1 destabilization in a process that dampens HY5 protein abundance.By regulating its accumulation,DET1 avoids HY5 association with hundreds of second-site genomic loci,which are also frequently targeted by the skotomorphogenic transcription factor PHYTOCHROME-INTERACTING FACTOR 3.Accordingly,ectopic HY5 chromatin enrichment favors local gene repression and can trigger fusca-like phenotypes.This study therefore shows that DET1-mediated regulation of COP1 stability tunes down the HY5 cistrome,avoiding hyper-photomorphogenic responses that might compromise plant viability.展开更多
Arabidopsis De-etiolated 1 （DET1） is one of the key repressors that maintain the etiolated state of seedlings in darkness. The plant hormone gibberellic acid （GA） also participates in this process, and plants defi...Arabidopsis De-etiolated 1 （DET1） is one of the key repressors that maintain the etiolated state of seedlings in darkness. The plant hormone gibberellic acid （GA） also participates in this process, and plants deficient in GA synthesis or signaling show a partially de.etiolated phenotype in darkness. However, how DET1 and the GA pathway work in concert in repressing photomorphogenesis remains largely unknown. In this study, we found that the abundance of DELLA proteins in detl-1 was increased in comparison with that in the wildtype plants. Mutation in DET1 changed the sensitivity of hypocotyl elongation of mutant seedlings to GA and paclobutrazol （PAC）, an inhibitor of GA synthesis. However, we did not find obvious differences between detl-1 and wild-type plants with regard to the bioactive GA content or the GA signaling upstream of DELLAs. Genetic data showed that removal of several DELLA proteins suppressed the detl-1 mutant phenotype more obviously than GA treatment, indicating that DET1 can regulate DELLA proteins via some other mechanisms. In addition, a large-scale transcriptomic analysis revealed that DET1 and DELLAs play antagonistic roles in regulating expression of photosynthetic and cell elongation-related genes in etiolated seedlings. Taken together, our results show that DET1 represses photomorphogenesis in darkness in part by reducing the abundance of DELLA proteins.展开更多
文摘从EMS诱变日本晴种子的M2代中筛选得到一个植株明显矮化、分蘖数急剧增多的突变体,命名为det1(dwarf and excessively-tillering1)。det1还显示出明显的穗型、粒型和育性等方面的生殖发育缺陷。遗传分析表明,det1受一对隐性基因控制。以det1与品种DZ60杂交建立了F2定位群体,利用微卫星标记分析F2群体中的突变体,将DET1基因定位在第6染色体长臂端,标记SSR2和SSR3之间约68kb的范围内。已知该区间内存在一个与水稻株高和分蘖有关的基因D3。测序结果表明,DET1与D3是同一个基因,编码一个LRR型的F-box蛋白。与d3相比,det1还显示了新的表型特征,推测可能是等位基因的不同突变位点造成的。det1/d3的表型特征提示,DET1/D3在调节水稻生长发育过程中具有多效性。因此,利用det1开展进一步的研究将有助于全面揭示DET1/D3的分子功能。
基金supported by a Ramon y Cajal(RYC-2014-16308)grant funded by the Ministerio de Economfa y Competitividad to S.F.Work by S.F.in F.B.’s lab was supported by the COST Action CA16212 INDEPTH(European Union)funded by the Agencia Estatal de Investigacion/Fondo Europeo de Desarollo Regional/European Union(BIO2016-80551-R and PID2019-105495GB-I00).+2 种基金supported by CNRS EPIPLANT Action(France)and funded by Agence Nationale de la Recherche grants ANR-10-LABX-54,ANR-18-CE13-0004-01,ANR-17-CE12-0026-02(France)by Velux Stiftung(Switzerland).B.G.G.is funded by President's International Fellowship Initiative postdoctoral fellowship(no.2020PB0082)the Chinese Academy of Sciences,and is the recipient of a Talent-Introduction grant(Chinese Postdoctoral International Exchange Program).
文摘DE-ETIOLATED 1(DET1)and CONSTITUTIVE PHOTOMORPHOGENESIS 1(COP1)are two essential repressors of Arabidopsis photomorphogenesis.These proteins can associate with CULLIN4 to form independent CRL4-based E3 ubiquitin ligases that mediate the degradation of several photomorphogenic transcription factors,including ELONGATED HYPOCOTYL 5(HY5),thereby controlling multiple gene-regulatory networks.Despite extensive biochemical and genetic analyses of their multi-subunit complexes,the functional links between DET1 and COP1 have long remained elusive.Here,we report that DET1 associates with COP1 in vivo,enhances COP1-HY5 interaction,and promotes COP1 destabilization in a process that dampens HY5 protein abundance.By regulating its accumulation,DET1 avoids HY5 association with hundreds of second-site genomic loci,which are also frequently targeted by the skotomorphogenic transcription factor PHYTOCHROME-INTERACTING FACTOR 3.Accordingly,ectopic HY5 chromatin enrichment favors local gene repression and can trigger fusca-like phenotypes.This study therefore shows that DET1-mediated regulation of COP1 stability tunes down the HY5 cistrome,avoiding hyper-photomorphogenic responses that might compromise plant viability.
基金This work was supported by grants to H.C. from the National Natural Science Foundation of China （31271294）, the National Program on Key Basic Research Project of China （973 Program： 2011CB100101）, the National High Technology Research and Development Program of China （863 Pro- gram： 2012AA10A304）, the Ministry of Agriculture of China （948 Program： 2011-G2B）, and State Key Laboratory of Protein and Plant Gene Research and grants to X.W.D. from the National Natural Science Foundation of China （31330048, U1031001）, the National Program on Key Basic Research Project of China （973 Program： 2012CB910900）, Peking-Tsinghua Center for Life Sciences, and State Key Laboratory of Protein and Plant Gene Research.ACKNOWLEDGMENTS We thank Renbo Yu, Jie Dong, and other laboratory members for their constructive discussion and help. No conflict of interest declared.
文摘Arabidopsis De-etiolated 1 （DET1） is one of the key repressors that maintain the etiolated state of seedlings in darkness. The plant hormone gibberellic acid （GA） also participates in this process, and plants deficient in GA synthesis or signaling show a partially de.etiolated phenotype in darkness. However, how DET1 and the GA pathway work in concert in repressing photomorphogenesis remains largely unknown. In this study, we found that the abundance of DELLA proteins in detl-1 was increased in comparison with that in the wildtype plants. Mutation in DET1 changed the sensitivity of hypocotyl elongation of mutant seedlings to GA and paclobutrazol （PAC）, an inhibitor of GA synthesis. However, we did not find obvious differences between detl-1 and wild-type plants with regard to the bioactive GA content or the GA signaling upstream of DELLAs. Genetic data showed that removal of several DELLA proteins suppressed the detl-1 mutant phenotype more obviously than GA treatment, indicating that DET1 can regulate DELLA proteins via some other mechanisms. In addition, a large-scale transcriptomic analysis revealed that DET1 and DELLAs play antagonistic roles in regulating expression of photosynthetic and cell elongation-related genes in etiolated seedlings. Taken together, our results show that DET1 represses photomorphogenesis in darkness in part by reducing the abundance of DELLA proteins.
