Nonsynonymous single nucleotide polymorphisms (nsSNPs) in coding regions can lead to amino acid changes that might alter the protein’s function and account for susceptibility to disease and altered drug/xenobiotic re...Nonsynonymous single nucleotide polymorphisms (nsSNPs) in coding regions can lead to amino acid changes that might alter the protein’s function and account for susceptibility to disease and altered drug/xenobiotic response. Many nsSNPs have been found in genes encoding human phase II metabolizing enzymes; however, there is little known about the relationship between the genotype and phenotype of nsSNPs in these enzymes. We have identified 923 validated nsSNPs in 104 human phase II enzyme genes from the Ensembl genome database and the NCBI SNP database. Using PolyPhen, Panther, and SNAP algorithms, 44%?59% of nsSNPs in phase II enzyme genes were predicted to have functional impacts on protein function. Predictions largely agree with the available experimental annotations. 68% of deleterious nsSNPs were correctly predicted as damaging. This study also identified many amino acids that are likely to be functionally critical, but have not yet been studied experimentally. There was significant concordance between the predicted results of Panther and PolyPhen, and between SNAP non-neutral predictions and PolyPhen scores. Evolutionarily non-neutral (destabilizing) amino acid substitutions are thought to be the pathogenetic basis for the alteration of phase II enzyme activity and to be associated with disease susceptibility and drug/xenobiotic toxicity. Furthermore, the molecular evolutionary patterns of phase II enzymes were characterized with regards to the predicted deleterious nsSNPs.展开更多
The effects of arbuscular mycorrhizal fungi（AMF） Glomus mosseae on the responses to elevated O3 in growth and nutrition of snap bean（Phaseolus vulgaris L.cv Guangzhouyuan） were investigated.Exposure was conducted ...The effects of arbuscular mycorrhizal fungi（AMF） Glomus mosseae on the responses to elevated O3 in growth and nutrition of snap bean（Phaseolus vulgaris L.cv Guangzhouyuan） were investigated.Exposure was conducted in growth chambers by using three O3 concentrations（20（CF）,80（CFO1） and 120 nL/L（CFO2）;8 hr/day for 75 days）.Results showed that elevated O3 slightly impacted overall mycorrhizal colonization,but significantly decreased the proportional frequency of hypha and increased the proportional frequency of spores and vesicles,suggesting that O3 had significant effects on mycorrhizal structure.Elevated O3 significantly decreased yield,dry mass and nutrient contents（N,P,K,Ca and Mg） in both non-mycorrhizal and mycorrhizal plants.However,significant interactive effects were found in most variables due to that the reduction by O3 in the mycorrhizal plants was less than that in the non-mycorrhizal plants.Additionally,AMF increased the concentrations of N,P,Ca,and Mg in shoot and root.It can be concluded that AMF alleviated detrimental effects of increasing O3 on host plant through improving plant nutrition and growth.展开更多
The palaeo-atmospheric CO2 concentration （pCOz） variation in the Yumen, Gansu Province during the middle Cretaceous has been reconstructed using the newly established plant photosynthetic gas exchange mechanistic mo...The palaeo-atmospheric CO2 concentration （pCOz） variation in the Yumen, Gansu Province during the middle Cretaceous has been reconstructed using the newly established plant photosynthetic gas exchange mechanistic model, and the results show that the pCO2 values are in the range of about 550 -808 ppmv. The present pCO2 values are higher than the pCO2 results （531-641 ppmv） of the previous study according to the Recent standardization of the stomatal ratio method, and much lower than the pCO2 results （882-1060 ppmv） according to the Carboniferous standardization of the stomatal ratio method. The present pCOz variation is not only within the error range of GEOCARB II and GEOCARB Ill but also is similar to the reconstructed results based on the biochemistry and carbon isotope models. Besides, the present Brachyphyllum specimens were collected from four consecutive horizons of the upper Zhonggou Formation of the Hanxia Section, and the reconstructed pCO2 exhibits the reconstructed pCO2 exhibits a decline trend during the late Aptian to early Albian. This decline variation is probably associated with the Oceanic Anoxic Events （OAElb） and the Cold snap event. With the combination of pCO2 during the Albian to Cenomanian recovered by the plant photosynthetic gas exchange mechanistic model, the pCO2 showed a prominent increase during the late Aptian to early Cenominian, which indicates a response to the greenhouse warming during the middle Cretaceous. Therefore, the mechanical model of the plant photosynthetic gas exchange shows a relatively strong accuracy in the reconstruction of thepCO2 and can reflect a strong relation between the atmospheric CO2 concentrations and climatic events.展开更多
Based on the theory of impact dynamics, the motion equations for a mooring line-floating body system before and after impact loading are established with consideration of the viscoelastic property of mooring lines. Th...Based on the theory of impact dynamics, the motion equations for a mooring line-floating body system before and after impact loading are established with consideration of the viscoelastic property of mooring lines. The factors that influence the taut-slack conditions of a mooring system are analyzed through classifying the taut-slack regions, which are defined by non-dimensional ratios of displacement, frequency, and damping of the system. The mooring system of Jip spar platform is analyzed, and the snap tension characteristics of mooring lines are given. The factors that influence the maximum tension in mooring lines, including the mass of the floating body, length of mooring lines, frequency and amplitude of external excitation, and pretension in mooting lines, are also analyzed through computing the dynamic response of system and parametric study. It is shown that the maximum tension increases with the increasing mass of the floating body, external excitation and pretension. Also, it is found that the influence of the non-dimensional ratio of damping increases with the increase of the pretension in mooring lines.展开更多
基金This program was financially supported in part by the National High Technology Research and Development Program of China(863 Program)(2004AA211172),The National Basic Research Program(973 Program)(2002CB111301),the Key Project of Chinese Ministry of Education(10418),and the program for Changjiang scholars and the innovative research teams in universities.
基金supported by the Major National Science and Technology Program (Grant No. 2008ZX10005-004)the Liaoning Education Depart-ment (Grant No. 2009A120)the China Postdoctoral Science Founda-tion (Grant Nos. 20080440019 and 200902069)
文摘Nonsynonymous single nucleotide polymorphisms (nsSNPs) in coding regions can lead to amino acid changes that might alter the protein’s function and account for susceptibility to disease and altered drug/xenobiotic response. Many nsSNPs have been found in genes encoding human phase II metabolizing enzymes; however, there is little known about the relationship between the genotype and phenotype of nsSNPs in these enzymes. We have identified 923 validated nsSNPs in 104 human phase II enzyme genes from the Ensembl genome database and the NCBI SNP database. Using PolyPhen, Panther, and SNAP algorithms, 44%?59% of nsSNPs in phase II enzyme genes were predicted to have functional impacts on protein function. Predictions largely agree with the available experimental annotations. 68% of deleterious nsSNPs were correctly predicted as damaging. This study also identified many amino acids that are likely to be functionally critical, but have not yet been studied experimentally. There was significant concordance between the predicted results of Panther and PolyPhen, and between SNAP non-neutral predictions and PolyPhen scores. Evolutionarily non-neutral (destabilizing) amino acid substitutions are thought to be the pathogenetic basis for the alteration of phase II enzyme activity and to be associated with disease susceptibility and drug/xenobiotic toxicity. Furthermore, the molecular evolutionary patterns of phase II enzymes were characterized with regards to the predicted deleterious nsSNPs.
基金supported by the National Natural Science Foundation of China(No.40701180,30670387)the Chinese Universities Scientific Fund(No.ZZ1106, ZD0904)the Project of Beijing Key Discipline and State Key Laboratory of Urban and Regional Ecology
文摘The effects of arbuscular mycorrhizal fungi（AMF） Glomus mosseae on the responses to elevated O3 in growth and nutrition of snap bean（Phaseolus vulgaris L.cv Guangzhouyuan） were investigated.Exposure was conducted in growth chambers by using three O3 concentrations（20（CF）,80（CFO1） and 120 nL/L（CFO2）;8 hr/day for 75 days）.Results showed that elevated O3 slightly impacted overall mycorrhizal colonization,but significantly decreased the proportional frequency of hypha and increased the proportional frequency of spores and vesicles,suggesting that O3 had significant effects on mycorrhizal structure.Elevated O3 significantly decreased yield,dry mass and nutrient contents（N,P,K,Ca and Mg） in both non-mycorrhizal and mycorrhizal plants.However,significant interactive effects were found in most variables due to that the reduction by O3 in the mycorrhizal plants was less than that in the non-mycorrhizal plants.Additionally,AMF increased the concentrations of N,P,Ca,and Mg in shoot and root.It can be concluded that AMF alleviated detrimental effects of increasing O3 on host plant through improving plant nutrition and growth.
基金the National Natural Science Foundation of China(No.41402007,41602023,40972025)the State Key Laboratory of Palaeobiology and Stratigraphy,Nanjing Institute of Geology and Palaeontology,CAS(No.153102)
文摘The palaeo-atmospheric CO2 concentration （pCOz） variation in the Yumen, Gansu Province during the middle Cretaceous has been reconstructed using the newly established plant photosynthetic gas exchange mechanistic model, and the results show that the pCO2 values are in the range of about 550 -808 ppmv. The present pCO2 values are higher than the pCO2 results （531-641 ppmv） of the previous study according to the Recent standardization of the stomatal ratio method, and much lower than the pCO2 results （882-1060 ppmv） according to the Carboniferous standardization of the stomatal ratio method. The present pCOz variation is not only within the error range of GEOCARB II and GEOCARB Ill but also is similar to the reconstructed results based on the biochemistry and carbon isotope models. Besides, the present Brachyphyllum specimens were collected from four consecutive horizons of the upper Zhonggou Formation of the Hanxia Section, and the reconstructed pCO2 exhibits the reconstructed pCO2 exhibits a decline trend during the late Aptian to early Albian. This decline variation is probably associated with the Oceanic Anoxic Events （OAElb） and the Cold snap event. With the combination of pCO2 during the Albian to Cenomanian recovered by the plant photosynthetic gas exchange mechanistic model, the pCO2 showed a prominent increase during the late Aptian to early Cenominian, which indicates a response to the greenhouse warming during the middle Cretaceous. Therefore, the mechanical model of the plant photosynthetic gas exchange shows a relatively strong accuracy in the reconstruction of thepCO2 and can reflect a strong relation between the atmospheric CO2 concentrations and climatic events.
基金supported by the National Natural Science Foundation of China (Grant Nos .50679051 and 50639030) the National High Technology Research and Development Program of China (863 Program, Grant No.2007AA09Z304)
文摘Based on the theory of impact dynamics, the motion equations for a mooring line-floating body system before and after impact loading are established with consideration of the viscoelastic property of mooring lines. The factors that influence the taut-slack conditions of a mooring system are analyzed through classifying the taut-slack regions, which are defined by non-dimensional ratios of displacement, frequency, and damping of the system. The mooring system of Jip spar platform is analyzed, and the snap tension characteristics of mooring lines are given. The factors that influence the maximum tension in mooring lines, including the mass of the floating body, length of mooring lines, frequency and amplitude of external excitation, and pretension in mooting lines, are also analyzed through computing the dynamic response of system and parametric study. It is shown that the maximum tension increases with the increasing mass of the floating body, external excitation and pretension. Also, it is found that the influence of the non-dimensional ratio of damping increases with the increase of the pretension in mooring lines.
