自从Chevreul ME发现糖尿病患者尿液中的糖是葡萄糖,近200年的时间,糖尿病被视为一组以葡萄糖慢性增高为特征的代谢性疾病,而体内广泛存在的核糖与糖尿病之间的关系却被忽略.研究发现核糖可以降低血葡萄糖浓度,曾报道糖尿病患者可以口...自从Chevreul ME发现糖尿病患者尿液中的糖是葡萄糖,近200年的时间,糖尿病被视为一组以葡萄糖慢性增高为特征的代谢性疾病,而体内广泛存在的核糖与糖尿病之间的关系却被忽略.研究发现核糖可以降低血葡萄糖浓度,曾报道糖尿病患者可以口服核糖.本实验室前期工作表明,核糖能够迅速与蛋白质发生非酶促糖基化,形成具有强烈细胞毒性的糖基化终末产物(advanced glycation end products,AGEs),引起细胞(包括神经细胞)死亡.进一步的实验证明,虽然在给小鼠注射核糖时,血葡萄糖浓度有所降低,但是糖基化血清蛋白和AGEs均显著升高,说明核糖浓度的升高更容易使机体发生非酶促糖基化反应,产生AGEs,从而造成危害.本文采用1-(4-羧基苯基)-3-甲基-5-吡唑酮(MOPBA)结合高效液相色谱,对明确诊断的2型糖尿病患者(n=30)和同龄健康人(n=30)尿核糖进行了定量分析,结果显示,MOPBA-核糖衍生物与尿核糖浓度之间呈线性相关(r2=0.999),回收率达99%.经质谱分析显示,HPLC分离的糖尿病患者尿样品中含569.19 u MOPBA衍生物峰(核糖,C27H29N4O10)和599.20 u MOPBA衍生物峰(葡萄糖,C28H31N4O11).2型糖尿病组尿核糖浓度(男性:(134.28±35.09)μmol/L;女性:(97.33±23.68)μmol/L)显著高于正常对照组(男性:(35.99±5.64)μmol/L;女性:(33.72±6.27)μmol/L)(P<0.001),同时,其尿葡萄糖浓度也显著高于正常对照(P<0.001).糖尿病患者尿核糖显著高于正常人的现象提示,2型糖尿病不但葡萄糖代谢异常,同时核糖代谢也发生了异常.展开更多
The method has been put forward to analyze the content of D-ribose obtained from glucose zymotic fluid. D-ribose was determined by HPLC, on a Sugar PakⅠcolumn, by using a refractive index detector and a mobile phase ...The method has been put forward to analyze the content of D-ribose obtained from glucose zymotic fluid. D-ribose was determined by HPLC, on a Sugar PakⅠcolumn, by using a refractive index detector and a mobile phase of pure water. We have also studied the separation conditions of sugars. It has many advantages, such as rapid analysis, high sensitivity and high precision and good reproducibility. The recovery of D-ribose were 95.5%—104%, RSD were less than 1 5%, the detection limit of D-Ribose was 50 ng.展开更多
文摘自从Chevreul ME发现糖尿病患者尿液中的糖是葡萄糖,近200年的时间,糖尿病被视为一组以葡萄糖慢性增高为特征的代谢性疾病,而体内广泛存在的核糖与糖尿病之间的关系却被忽略.研究发现核糖可以降低血葡萄糖浓度,曾报道糖尿病患者可以口服核糖.本实验室前期工作表明,核糖能够迅速与蛋白质发生非酶促糖基化,形成具有强烈细胞毒性的糖基化终末产物(advanced glycation end products,AGEs),引起细胞(包括神经细胞)死亡.进一步的实验证明,虽然在给小鼠注射核糖时,血葡萄糖浓度有所降低,但是糖基化血清蛋白和AGEs均显著升高,说明核糖浓度的升高更容易使机体发生非酶促糖基化反应,产生AGEs,从而造成危害.本文采用1-(4-羧基苯基)-3-甲基-5-吡唑酮(MOPBA)结合高效液相色谱,对明确诊断的2型糖尿病患者(n=30)和同龄健康人(n=30)尿核糖进行了定量分析,结果显示,MOPBA-核糖衍生物与尿核糖浓度之间呈线性相关(r2=0.999),回收率达99%.经质谱分析显示,HPLC分离的糖尿病患者尿样品中含569.19 u MOPBA衍生物峰(核糖,C27H29N4O10)和599.20 u MOPBA衍生物峰(葡萄糖,C28H31N4O11).2型糖尿病组尿核糖浓度(男性:(134.28±35.09)μmol/L;女性:(97.33±23.68)μmol/L)显著高于正常对照组(男性:(35.99±5.64)μmol/L;女性:(33.72±6.27)μmol/L)(P<0.001),同时,其尿葡萄糖浓度也显著高于正常对照(P<0.001).糖尿病患者尿核糖显著高于正常人的现象提示,2型糖尿病不但葡萄糖代谢异常,同时核糖代谢也发生了异常.
文摘The method has been put forward to analyze the content of D-ribose obtained from glucose zymotic fluid. D-ribose was determined by HPLC, on a Sugar PakⅠcolumn, by using a refractive index detector and a mobile phase of pure water. We have also studied the separation conditions of sugars. It has many advantages, such as rapid analysis, high sensitivity and high precision and good reproducibility. The recovery of D-ribose were 95.5%—104%, RSD were less than 1 5%, the detection limit of D-Ribose was 50 ng.
