Cutaneous regeneration at the wound site involves several intricate and dynamic processes which require a series of coordinated interactions implicating various cell types,growth factors,extracellular matrix(ECM),nerv...Cutaneous regeneration at the wound site involves several intricate and dynamic processes which require a series of coordinated interactions implicating various cell types,growth factors,extracellular matrix(ECM),nerves,and blood vessels.Mesenchymal stromal cells(MSCs)take part in all the skin wound healing stages playing active and beneficial roles in animal models and humans.Exosomes,which are among the key products MSCs release,mimic the effects of parental MSCs.They can shuttle various effector proteins,messenger RNA(mRNA)and microRNAs(miRNAs)to modulate the activity of recipient cells,playing important roles in wound healing.Moreover,using exosomes avoids many risks associated with cell transplantation.Therefore,as a novel type of cell-free therapy,MSC-exosome-mediated administration may be safer and more efficient than whole cell.In this review,we provide a comprehensive understanding of the latest studies and observations on the role of MSC-exosome therapy in wound healing and cutaneous regeneration.In addition,we address the hypothesis of MSCs microenvironment extracellular vesicles(MSCs-MEVs)or MSCs microenvironment exosomes(MSCs-MExos)that need to take stock of and solved urgently in the related research about MSC-exosomes therapeutic applications.This review can inspire investigators to explore new research directions of MSC-exosome therapy in cutaneous repair and regeneration.展开更多
Extrusion-based bioprinting (EBB) holds potential for regenerative medicine. However, the widely-used bioinks of EBB exhibit some limitations for skin regeneration, such as unsatisfactory bio-physical (i.e., mechanica...Extrusion-based bioprinting (EBB) holds potential for regenerative medicine. However, the widely-used bioinks of EBB exhibit some limitations for skin regeneration, such as unsatisfactory bio-physical (i.e., mechanical, structural, biodegradable) properties and compromised cellular compatibilities, and the EBB-based bioinks with therapeutic effects targeting cutaneous wounds still remain largely undiscussed. In this review, the printability considerations for skin bioprinting were discussed. Then, current strategies for improving the physical properties of bioinks and for reinforcing bioinks in EBB approaches were introduced, respectively. Notably, we highlighted the applications and effects of current EBB-based bioinks on wound healing, wound scar formation, vasculari-zation and the regeneration of skin appendages (i.e., sweat glands and hair follicles) and discussed the challenges and future perspectives. This review aims to provide an overall view of the applications, challenges and promising solutions about the EBB-based bioinks for cutaneous wound healing and skin regeneration.展开更多
Objective:To evaluate the in vitro and in vivo efficacy of quercetin and its immunomodulatory and anti-oxidative activity against Leishmania major(L.major).Methods:L.major promastigotes and amastigotes were incubated ...Objective:To evaluate the in vitro and in vivo efficacy of quercetin and its immunomodulatory and anti-oxidative activity against Leishmania major(L.major).Methods:L.major promastigotes and amastigotes were incubated with different concentrations of quercetin to estimate EC_(50).For in vivo study,the base of tails of mice was infected with L.major.After developing ulcers in the inoculation site,mice were treated with 50 mg/kg quercetin orally for 28 consecutive days.The wound-healing potential of quercetin was evaluated by histopathological analysis of tissue sections stained by hematoxylin and eosin as well as Masson's trichrome.In addition,the levels of tumor necrosis factor-α,interleukin-6,malondialdehyde,and adiponectin,the ferric reducing ability of plasma,as well as superoxide dismutase and glutathione peroxidase activities were measured.Results:The EC_(50)values of quercetin against L.major promastigotes and intracellular amastigotes were 0.27 and 0.85μM,respectively.Histopathological analysis showed that fewer inflammatory cells,more fibroblasts,and more collagen deposition were observed in tissue sections of quercetin-treated mice.In addition,treatment with quercetin markedly increased glutathione peroxidase activity,the ferric reducing ability of plasma and adiponectin levels while decreasing malondialdehyde,interleukin-6,and tumor necrosis factor-αlevels.Conclusions:Quercetin shows anti-leishmanial activity,immunomodulatory,anti-oxidative,and anti-inflammatory effects.Therefore,it may be further explored as an effective drug in treating leishmaniasis.展开更多
Objective:To explore the effect of the Xian Fang Huo Ming Yin(XFHM)for treating cutaneous infections and promoting wound healing in patients with perianal abscesses.Methods:Sixty-one patients with perianal abscesses w...Objective:To explore the effect of the Xian Fang Huo Ming Yin(XFHM)for treating cutaneous infections and promoting wound healing in patients with perianal abscesses.Methods:Sixty-one patients with perianal abscesses who were admitted to our hospital(Xinghua City People’s Hospital)from May 2022 to May 2023 were selected and randomly divided into two groups,a control group(30 cases)and a study group(31 cases).Both groups received surgical treatment.The control group received conventional treatment and warm water fumigation,sitz bath,and surgical dressing change after surgery,while the research group received XFHM based on the control group.XFHM was taken orally and replaced with warm water for fumigation and sitz bathing.Both groups received treatment for 4 weeks but discontinued sitz bathing after 2 weeks.Various clinical indicators between the two groups were compared.Results:The total clinical effective rate and wound recovery rate of the study group were higher than that of the control group.There were differences in the wound pain scores,surrounding tissue edema,and wound secretions at different time points.Both groups experienced wound pain.The scores of wound pain,surrounding tissue edema,and wound secretions of the study group were lower than those of the control group,7 and 14 days after surgery.The serum interleukin 6(IL-6),tumor necrosis factor-alpha(TNF-α)levels,and pH values of the study group were lower than those of the control group 10 days after surgery(P<0.05).Conclusion:The application of XFHM for treating cutaneous infections and promoting wound healing in patients with perianal abscesses improved the treatment outcome,alleviated clinical symptoms,and promoted healing.展开更多
Wound healing refers to the healing process that occurs after the skin and other tissues are separated or damaged by internal or external forces.It is a complex combination of tissue regeneration,granulation tissue hy...Wound healing refers to the healing process that occurs after the skin and other tissues are separated or damaged by internal or external forces.It is a complex combination of tissue regeneration,granulation tissue hyperplasia,and scar formation,and shows the synergistic effects of these processes.After skin damage,the environment around the wound and the cells at site of the damage respond immediately,and a range of cytokines and growth factors are released.In cutaneous injury,extracellular vesicle(EV)signaling plays a vital role in the healing process via paracrine and endocrine mechanisms.EVs are natural intercellular and inter-organ communication tools that carry various bioactive substances for message exchange.Stem cells and stem cell EVs facilitate tissue repair,showing promising potential in regenerative medicine.Nevertheless,EVs derived from specific skin tissue cells,such as epidermal cells,fibroblasts,vascular endothelial cells and inflammatory cells,also play important roles in cutaneous tissue repair.Here,we describe the characteristics of wound healing,concentrating on the production and functions of EVs derived from specific skin cells,and provide new ideas for wound therapy using EVs.展开更多
Regenerative medicine has brought about refreshing new thinking about age old problems. However, some problems remain mostly untouched and are not being addressed. A point in question is the track of scar tissue left ...Regenerative medicine has brought about refreshing new thinking about age old problems. However, some problems remain mostly untouched and are not being addressed. A point in question is the track of scar tissue left behind post-operatively, which reveals the surgeon’s line of invasive incision. This confers on the patient an adverse psychological reminder and burden for the rest of his/her life. Most patients cannot afford corrective plastic surgery to ameliorate this skin defect. This paper seeks to ask whether biomedical scientists could play a role in arriving at a more pleasing cosmetic result, using a simple cell culture procedure of isolating un-manipulated autologous primary epidermal and dermal cells from a small skin tissue segment in close proximity to the surgeon’s incision line.展开更多
Background:The present health economic evaluation in Afghanistan aims to support public health decision makers and health care managers to allocate resources efficiently to appropriate treatments for cutaneous leishma...Background:The present health economic evaluation in Afghanistan aims to support public health decision makers and health care managers to allocate resources efficiently to appropriate treatments for cutaneous leishmaniasis(CL)elicited by Leishmania tropica or Leishmania major.Methods:A decision tree was used to analyse the cost and the effectiveness of two wound care regimens versus intra-lesional antimony in CL patients in Afghanistan.Costs were collected from a societal perspective.Effectiveness was measured in wound free days.The incremental cost-effectiveness ratio(ICER)and incremental net monetary benefit(NMB)were calculated.The model was parameterized with baseline parameters,sensitivity ranges,and parameter distributions.Finally,the model was simulated and results were evaluated with deterministic and probability sensitivity analyses.Final outcomes were the efficiency of the regimens and a budget impact analysis in the context of Afghanistan.Results:Average costs per patients were US$11(SE=0.016)(Group I:Intra-dermal Sodium Stibogluconate[IL SSG]),US$16(SE=7.58)(Group II:Electro-thermo-debridement[ETD]+Moist wound treatment[MWT])and US$25(SE=0.48)(Group III:MWT)in patients with a single chronic CL ulcer.From a societal perspective the budget impact analysis shows that the regimens’drug costs are lower than indirect disease cost.Average effectiveness in wound free days are 177(SE=0.36)in Group II,147(SE=0.33)in Group III,and 129(SE=0.27)in Group I.The ICER of Group II versus Group I was US$0.09 and Group III versus Group I US$0.77,which is very costeffective with a willingness-to-pay threshold of US$2 per wound free day.Within a Monte-Carlo probabilistic sensitivity analysis Group II was cost-effective in 80%of the cases starting at a willingness-to-pay of 80 cent per wound free day.Conclusions:Group II provided the most cost-effective treatment.The non-treatment alternative is not an option in the management of chronic CL ulcers.MWT of Group III should at least be practiced.The costeffectiveness of Grou展开更多
基金funded by National Natural Science Foundation of China(81871570,81560313).
文摘Cutaneous regeneration at the wound site involves several intricate and dynamic processes which require a series of coordinated interactions implicating various cell types,growth factors,extracellular matrix(ECM),nerves,and blood vessels.Mesenchymal stromal cells(MSCs)take part in all the skin wound healing stages playing active and beneficial roles in animal models and humans.Exosomes,which are among the key products MSCs release,mimic the effects of parental MSCs.They can shuttle various effector proteins,messenger RNA(mRNA)and microRNAs(miRNAs)to modulate the activity of recipient cells,playing important roles in wound healing.Moreover,using exosomes avoids many risks associated with cell transplantation.Therefore,as a novel type of cell-free therapy,MSC-exosome-mediated administration may be safer and more efficient than whole cell.In this review,we provide a comprehensive understanding of the latest studies and observations on the role of MSC-exosome therapy in wound healing and cutaneous regeneration.In addition,we address the hypothesis of MSCs microenvironment extracellular vesicles(MSCs-MEVs)or MSCs microenvironment exosomes(MSCs-MExos)that need to take stock of and solved urgently in the related research about MSC-exosomes therapeutic applications.This review can inspire investigators to explore new research directions of MSC-exosome therapy in cutaneous repair and regeneration.
基金This study was supported by the Beijing Natural Science Foundation(7204306)National Natural Science Foundation of China(82002056,81830064,81721092,32000969,81974019)+9 种基金National Key Research and Development Program of China(2018YFA0108700,2017YFA0105602)NSFC Projects of International Cooperation and Exchanges(81720108004)China Postdoctoral Science Foundation(2020M673672)Key Support Program for Growth Factor Research(SZYZ-TR-03)Chinese PLA General Hospital for Military Medical Innovation Research Project(CX19026)the CAMS Innovation Fund for Medical Sciences(CIFMS,2019-I2M-5-059)the Military Medical Research and Development Projects(AWS17J005)the Research Team Project of Natural Science Foundation of Guangdong Province of China(2017A030312007)the key program of guangzhou science research plan(201904020047)The Special Project of Dengfeng Program of Guangdong Provincial People’s Hospital(DFJH201812,KJ012019119,KJ012019423).
文摘Extrusion-based bioprinting (EBB) holds potential for regenerative medicine. However, the widely-used bioinks of EBB exhibit some limitations for skin regeneration, such as unsatisfactory bio-physical (i.e., mechanical, structural, biodegradable) properties and compromised cellular compatibilities, and the EBB-based bioinks with therapeutic effects targeting cutaneous wounds still remain largely undiscussed. In this review, the printability considerations for skin bioprinting were discussed. Then, current strategies for improving the physical properties of bioinks and for reinforcing bioinks in EBB approaches were introduced, respectively. Notably, we highlighted the applications and effects of current EBB-based bioinks on wound healing, wound scar formation, vasculari-zation and the regeneration of skin appendages (i.e., sweat glands and hair follicles) and discussed the challenges and future perspectives. This review aims to provide an overall view of the applications, challenges and promising solutions about the EBB-based bioinks for cutaneous wound healing and skin regeneration.
基金The study was financially supported by INSF(grant number 96006039).
文摘Objective:To evaluate the in vitro and in vivo efficacy of quercetin and its immunomodulatory and anti-oxidative activity against Leishmania major(L.major).Methods:L.major promastigotes and amastigotes were incubated with different concentrations of quercetin to estimate EC_(50).For in vivo study,the base of tails of mice was infected with L.major.After developing ulcers in the inoculation site,mice were treated with 50 mg/kg quercetin orally for 28 consecutive days.The wound-healing potential of quercetin was evaluated by histopathological analysis of tissue sections stained by hematoxylin and eosin as well as Masson's trichrome.In addition,the levels of tumor necrosis factor-α,interleukin-6,malondialdehyde,and adiponectin,the ferric reducing ability of plasma,as well as superoxide dismutase and glutathione peroxidase activities were measured.Results:The EC_(50)values of quercetin against L.major promastigotes and intracellular amastigotes were 0.27 and 0.85μM,respectively.Histopathological analysis showed that fewer inflammatory cells,more fibroblasts,and more collagen deposition were observed in tissue sections of quercetin-treated mice.In addition,treatment with quercetin markedly increased glutathione peroxidase activity,the ferric reducing ability of plasma and adiponectin levels while decreasing malondialdehyde,interleukin-6,and tumor necrosis factor-αlevels.Conclusions:Quercetin shows anti-leishmanial activity,immunomodulatory,anti-oxidative,and anti-inflammatory effects.Therefore,it may be further explored as an effective drug in treating leishmaniasis.
文摘Objective:To explore the effect of the Xian Fang Huo Ming Yin(XFHM)for treating cutaneous infections and promoting wound healing in patients with perianal abscesses.Methods:Sixty-one patients with perianal abscesses who were admitted to our hospital(Xinghua City People’s Hospital)from May 2022 to May 2023 were selected and randomly divided into two groups,a control group(30 cases)and a study group(31 cases).Both groups received surgical treatment.The control group received conventional treatment and warm water fumigation,sitz bath,and surgical dressing change after surgery,while the research group received XFHM based on the control group.XFHM was taken orally and replaced with warm water for fumigation and sitz bathing.Both groups received treatment for 4 weeks but discontinued sitz bathing after 2 weeks.Various clinical indicators between the two groups were compared.Results:The total clinical effective rate and wound recovery rate of the study group were higher than that of the control group.There were differences in the wound pain scores,surrounding tissue edema,and wound secretions at different time points.Both groups experienced wound pain.The scores of wound pain,surrounding tissue edema,and wound secretions of the study group were lower than those of the control group,7 and 14 days after surgery.The serum interleukin 6(IL-6),tumor necrosis factor-alpha(TNF-α)levels,and pH values of the study group were lower than those of the control group 10 days after surgery(P<0.05).Conclusion:The application of XFHM for treating cutaneous infections and promoting wound healing in patients with perianal abscesses improved the treatment outcome,alleviated clinical symptoms,and promoted healing.
基金supported by the National Natural Science Youth Foundation of China(Grant 82001975)the Natural Science Youth Foundation of the Jiangsu Province(Grant BK20190841)+4 种基金Jiangsu Province‘Entrepreneurship and Innovation Program’-Entrepreneurship and innovation Doctoral category.Top Talent Support Program for young and middle-aged people of Wuxi Health Committee(HB2020108)The Foundation of Clinical Science and Technology of Wuxi(No.Q202059)The Natural Science Youth Foundation of the Jiangsu Province(Grant BK20210074)The Innovation Fund on Medicine and Education Connection of Jiangsu University(JDY2022017)The Innovation Project for Graduate Student Research of Jiangsu Province(Grant No.KYCX21_3406).
文摘Wound healing refers to the healing process that occurs after the skin and other tissues are separated or damaged by internal or external forces.It is a complex combination of tissue regeneration,granulation tissue hyperplasia,and scar formation,and shows the synergistic effects of these processes.After skin damage,the environment around the wound and the cells at site of the damage respond immediately,and a range of cytokines and growth factors are released.In cutaneous injury,extracellular vesicle(EV)signaling plays a vital role in the healing process via paracrine and endocrine mechanisms.EVs are natural intercellular and inter-organ communication tools that carry various bioactive substances for message exchange.Stem cells and stem cell EVs facilitate tissue repair,showing promising potential in regenerative medicine.Nevertheless,EVs derived from specific skin tissue cells,such as epidermal cells,fibroblasts,vascular endothelial cells and inflammatory cells,also play important roles in cutaneous tissue repair.Here,we describe the characteristics of wound healing,concentrating on the production and functions of EVs derived from specific skin cells,and provide new ideas for wound therapy using EVs.
文摘Regenerative medicine has brought about refreshing new thinking about age old problems. However, some problems remain mostly untouched and are not being addressed. A point in question is the track of scar tissue left behind post-operatively, which reveals the surgeon’s line of invasive incision. This confers on the patient an adverse psychological reminder and burden for the rest of his/her life. Most patients cannot afford corrective plastic surgery to ameliorate this skin defect. This paper seeks to ask whether biomedical scientists could play a role in arriving at a more pleasing cosmetic result, using a simple cell culture procedure of isolating un-manipulated autologous primary epidermal and dermal cells from a small skin tissue segment in close proximity to the surgeon’s incision line.
基金German Federal Ministry for Education and Research(Grant No.AFG 08/002).
文摘Background:The present health economic evaluation in Afghanistan aims to support public health decision makers and health care managers to allocate resources efficiently to appropriate treatments for cutaneous leishmaniasis(CL)elicited by Leishmania tropica or Leishmania major.Methods:A decision tree was used to analyse the cost and the effectiveness of two wound care regimens versus intra-lesional antimony in CL patients in Afghanistan.Costs were collected from a societal perspective.Effectiveness was measured in wound free days.The incremental cost-effectiveness ratio(ICER)and incremental net monetary benefit(NMB)were calculated.The model was parameterized with baseline parameters,sensitivity ranges,and parameter distributions.Finally,the model was simulated and results were evaluated with deterministic and probability sensitivity analyses.Final outcomes were the efficiency of the regimens and a budget impact analysis in the context of Afghanistan.Results:Average costs per patients were US$11(SE=0.016)(Group I:Intra-dermal Sodium Stibogluconate[IL SSG]),US$16(SE=7.58)(Group II:Electro-thermo-debridement[ETD]+Moist wound treatment[MWT])and US$25(SE=0.48)(Group III:MWT)in patients with a single chronic CL ulcer.From a societal perspective the budget impact analysis shows that the regimens’drug costs are lower than indirect disease cost.Average effectiveness in wound free days are 177(SE=0.36)in Group II,147(SE=0.33)in Group III,and 129(SE=0.27)in Group I.The ICER of Group II versus Group I was US$0.09 and Group III versus Group I US$0.77,which is very costeffective with a willingness-to-pay threshold of US$2 per wound free day.Within a Monte-Carlo probabilistic sensitivity analysis Group II was cost-effective in 80%of the cases starting at a willingness-to-pay of 80 cent per wound free day.Conclusions:Group II provided the most cost-effective treatment.The non-treatment alternative is not an option in the management of chronic CL ulcers.MWT of Group III should at least be practiced.The costeffectiveness of Grou
