目的:采用网络药理学方法对菟丝子主要活性成分的作用机制进行分析,探讨其成分、多靶点、靶点相关信号通路及生物学过程的相互关系。方法:首先借助中药系统药理学分析平台(Chinese medicine system pharmacology analysis platform,TCM...目的:采用网络药理学方法对菟丝子主要活性成分的作用机制进行分析,探讨其成分、多靶点、靶点相关信号通路及生物学过程的相互关系。方法:首先借助中药系统药理学分析平台(Chinese medicine system pharmacology analysis platform,TCMSP)筛选出菟丝子中的活性成分及相关作用靶点,然后通过Uniprot数据库对提取靶点进行筛选与转化,并利用CTD网络在线分析平台获取与靶点相关的疾病,利用Cytoscape 3.6.1及其插件ClueGO构建“活性成分-靶点”“靶点-疾病”“靶点-生物学通路”网络图,最后利用Cytoscape 3.6.1中的Network Analyzer插件分析网络图,获取相关拓扑异构信息,建立菟丝子成分-靶点-通路网络模型。结果:筛选得到菟丝子8个活性成分,涉及靶点118个,靶点相关疾病516种;对所得靶点进行GO分析,再经Kappa算法聚类,共获得79类相关生物学过程和57类信号相关信号通路。结论:初步探讨菟丝子主要活性成分的作用机制,为深入研究菟丝子的药效作用及其机制提供参考。展开更多
AIM: To investigate the anti-inflammatory activities of the semen extract of Cuscuta chinensis Lam.(Cuscutae Semen; CS) on the production of inflammatory mediators, nitric oxide(NO), prostaglandin 2(PGE2), and proinfl...AIM: To investigate the anti-inflammatory activities of the semen extract of Cuscuta chinensis Lam.(Cuscutae Semen; CS) on the production of inflammatory mediators, nitric oxide(NO), prostaglandin 2(PGE2), and proinflammatory cytokines in lipopolysaccharide(LPS)-stimulated BV-2 microglia. METHOD: BV-2 cells were treated with CS extract for 30 min, and then stimulated with LPS or without for 24 h. The levels of NO, PGE2 and proinflammatory cytokines were measured by Griess assay and ELISA. The expression of inducible nitric oxide synthase(iNOS), and cyclooxygenase(COX)-2 mRNA and protein was determined by RT-PCR and Western blot, respectively. The phosphorylation of extracellular signal-regulated kinase 1/2(ERK1/2), Jun N-terminal kinase(JNK), and p38 mitogen-activated protein kinase(MAPK), and the nuclear expression of nuclear factor(NF)-κB p65 were investigated by Western blot analysis. RESULTS: CS extract significantly decreased the production of NO and PGE2 by suppressing the expression of iNOS and COX-2 in activated microglia. CS extract decreased the production of TNF-α, IL-1β, and IL-6 by down-regulating their transcription levels. In addition, CS extract suppressed the phosphorylation of ERK1/2, JNK, and p38 MAPK, and the nuclear translocation of NF-κB p65 in activated microglia. CONCLUSION: These results indicate that CS extract is capable of suppressing the inflammatory response by microglia activation, suggesting that CS extract has potential in the treatment of brain inflammation.展开更多
文摘目的:采用网络药理学方法对菟丝子主要活性成分的作用机制进行分析,探讨其成分、多靶点、靶点相关信号通路及生物学过程的相互关系。方法:首先借助中药系统药理学分析平台(Chinese medicine system pharmacology analysis platform,TCMSP)筛选出菟丝子中的活性成分及相关作用靶点,然后通过Uniprot数据库对提取靶点进行筛选与转化,并利用CTD网络在线分析平台获取与靶点相关的疾病,利用Cytoscape 3.6.1及其插件ClueGO构建“活性成分-靶点”“靶点-疾病”“靶点-生物学通路”网络图,最后利用Cytoscape 3.6.1中的Network Analyzer插件分析网络图,获取相关拓扑异构信息,建立菟丝子成分-靶点-通路网络模型。结果:筛选得到菟丝子8个活性成分,涉及靶点118个,靶点相关疾病516种;对所得靶点进行GO分析,再经Kappa算法聚类,共获得79类相关生物学过程和57类信号相关信号通路。结论:初步探讨菟丝子主要活性成分的作用机制,为深入研究菟丝子的药效作用及其机制提供参考。
基金supported by the"Study of Aging-control by Energy Metabolism based on Oriental Medicine(No.K12101)"funded by"KM-Based Herbal Drug Research Group"of Korea Institute of Oriental Medicine,Republic of Korea
文摘AIM: To investigate the anti-inflammatory activities of the semen extract of Cuscuta chinensis Lam.(Cuscutae Semen; CS) on the production of inflammatory mediators, nitric oxide(NO), prostaglandin 2(PGE2), and proinflammatory cytokines in lipopolysaccharide(LPS)-stimulated BV-2 microglia. METHOD: BV-2 cells were treated with CS extract for 30 min, and then stimulated with LPS or without for 24 h. The levels of NO, PGE2 and proinflammatory cytokines were measured by Griess assay and ELISA. The expression of inducible nitric oxide synthase(iNOS), and cyclooxygenase(COX)-2 mRNA and protein was determined by RT-PCR and Western blot, respectively. The phosphorylation of extracellular signal-regulated kinase 1/2(ERK1/2), Jun N-terminal kinase(JNK), and p38 mitogen-activated protein kinase(MAPK), and the nuclear expression of nuclear factor(NF)-κB p65 were investigated by Western blot analysis. RESULTS: CS extract significantly decreased the production of NO and PGE2 by suppressing the expression of iNOS and COX-2 in activated microglia. CS extract decreased the production of TNF-α, IL-1β, and IL-6 by down-regulating their transcription levels. In addition, CS extract suppressed the phosphorylation of ERK1/2, JNK, and p38 MAPK, and the nuclear translocation of NF-κB p65 in activated microglia. CONCLUSION: These results indicate that CS extract is capable of suppressing the inflammatory response by microglia activation, suggesting that CS extract has potential in the treatment of brain inflammation.
