Curculigo(仙茅)is also called“Only Thatch Root,”“Ground Palm,”and“Brahman Ginseng.”The name of the“Only Thatch Root”vividly describes its shape characteristics.The nickname“Ground Palm”figuratively shows its...Curculigo(仙茅)is also called“Only Thatch Root,”“Ground Palm,”and“Brahman Ginseng.”The name of the“Only Thatch Root”vividly describes its shape characteristics.The nickname“Ground Palm”figuratively shows its growth environment and form feature.The another name“Brahman Ginseng”emphasizes the source,the value,and the efficiency of the immortal grass The name of“Curculigo”fully expresses its function feature and the application to the ancient regimen culture.Curculigo is not only a sacrificial offering often used by Brahmins to pray for good fortune and dispel disasters,but also used as a special medicine for nobles to live longer.After its introduction into China,Curculigo has been widely applied in both religious development and health preservation.展开更多
目的:研究仙茅提取物对小鼠巨噬细胞株RAW264.7的诱导活化作用。方法:应用水提醇沉方式制备仙茅提取物,其中多糖含量为50%。将不同浓度的仙茅提取物(31.25~500μg/ml)作用于RAW264.7细胞36h后,采用流式细胞术检测膜表面模式识别受体TLR...目的:研究仙茅提取物对小鼠巨噬细胞株RAW264.7的诱导活化作用。方法:应用水提醇沉方式制备仙茅提取物,其中多糖含量为50%。将不同浓度的仙茅提取物(31.25~500μg/ml)作用于RAW264.7细胞36h后,采用流式细胞术检测膜表面模式识别受体TLR2/4和MR的表达;实时荧光定量PCR(RT-PCR)检测细胞i NOS和TNF-α的mRNA表达水平;收集培养上清液,用ELISA法测定中TNF-α含量,Griess法检测NO的含量。结果:仙茅提取物的剂量在500μg/ml以下时,对细胞增殖影响不明显;与空白对照组相比,浓度高于62.5μg/ml的仙茅提取物刺激RAW264.7细胞可使TNF-a、i NOS mRNA表达量显著升高,促进细胞分泌活性因子TNF-α和NO;仙茅提取物可以有效增加RAW264.7细胞表面的模式识别受体的表达,TLR2由空白组的31.25±5.19上升到39.82±4.53,TLR4由空白组的6.41±2.44上升到24.34±5.22,MR由空白组的7.35±3.54上升到36.24±9.53,且均呈现剂量-反应关系。结论:仙茅提取物可能通过影响巨噬细胞膜表面部分模式识别受体的表达使TNF-α和NO的分泌量增多,诱导巨噬细胞活化,具有潜在的免疫调节价值,此作用与仙茅传统用于治疗虚劳内伤、现代用于免疫调节的临床应用有关。展开更多
文摘Curculigo(仙茅)is also called“Only Thatch Root,”“Ground Palm,”and“Brahman Ginseng.”The name of the“Only Thatch Root”vividly describes its shape characteristics.The nickname“Ground Palm”figuratively shows its growth environment and form feature.The another name“Brahman Ginseng”emphasizes the source,the value,and the efficiency of the immortal grass The name of“Curculigo”fully expresses its function feature and the application to the ancient regimen culture.Curculigo is not only a sacrificial offering often used by Brahmins to pray for good fortune and dispel disasters,but also used as a special medicine for nobles to live longer.After its introduction into China,Curculigo has been widely applied in both religious development and health preservation.
文摘目的:研究仙茅提取物对小鼠巨噬细胞株RAW264.7的诱导活化作用。方法:应用水提醇沉方式制备仙茅提取物,其中多糖含量为50%。将不同浓度的仙茅提取物(31.25~500μg/ml)作用于RAW264.7细胞36h后,采用流式细胞术检测膜表面模式识别受体TLR2/4和MR的表达;实时荧光定量PCR(RT-PCR)检测细胞i NOS和TNF-α的mRNA表达水平;收集培养上清液,用ELISA法测定中TNF-α含量,Griess法检测NO的含量。结果:仙茅提取物的剂量在500μg/ml以下时,对细胞增殖影响不明显;与空白对照组相比,浓度高于62.5μg/ml的仙茅提取物刺激RAW264.7细胞可使TNF-a、i NOS mRNA表达量显著升高,促进细胞分泌活性因子TNF-α和NO;仙茅提取物可以有效增加RAW264.7细胞表面的模式识别受体的表达,TLR2由空白组的31.25±5.19上升到39.82±4.53,TLR4由空白组的6.41±2.44上升到24.34±5.22,MR由空白组的7.35±3.54上升到36.24±9.53,且均呈现剂量-反应关系。结论:仙茅提取物可能通过影响巨噬细胞膜表面部分模式识别受体的表达使TNF-α和NO的分泌量增多,诱导巨噬细胞活化,具有潜在的免疫调节价值,此作用与仙茅传统用于治疗虚劳内伤、现代用于免疫调节的临床应用有关。
