The revolutionary improvement of hardware and algorithm in cryogenic electron microscopy(cryo-EM)has made it a routine method to obtain structures of macromolecules at near-atomic resolution.Nevertheless,this techniqu...The revolutionary improvement of hardware and algorithm in cryogenic electron microscopy(cryo-EM)has made it a routine method to obtain structures of macromolecules at near-atomic resolution.Nevertheless,this technique still faces many challenges.The structure-solving efficiency of cryo-EM can be significantly reduced by the biomolecules'denaturation on the air–water interfaces,the preferred orientation,strong background noise from supporting films and particle motion,and so forth.To overcome these problems,nanomaterials with ultrahigh electronic conductivity and ultrathin thickness are explored as promising cryo-EM specimen supporting films.Herein,we summarize the structural engineering of graphene,for example,surface and interface modification,as supporting films for grids and the application on high-resolution cryo-EM and discuss potential future perspectives.展开更多
Direct absorption spectra of the 2v3 band of methane （CH4） from 6038 to 6050 cm 1 were studied at different low temperatures using a newly developed cryogenic cell in combination with a distributed feedback （DFB） ...Direct absorption spectra of the 2v3 band of methane （CH4） from 6038 to 6050 cm 1 were studied at different low temperatures using a newly developed cryogenic cell in combination with a distributed feedback （DFB） diode laser. The cryogenic cell can operate at any stabilized temperature ranging from room temperature down to 100 K with temperature fluctuation less than =t=1 K within 1 hour. In the present work, the CH4 spectra in the range of 6038-6050 cm-1 were recorded at 296, 266, 248, 223, 198, and 176 K. The lower state energy Ett and the rotational assignment of the angular momentum J were determined by a ＂2-low-temperature spectra method＂ using the spectra recorded at 198 and 176 K. The results were compared with the data from the GOSAT and the recently reported results from Campargue and co-workers using two spectra measured at room temperature and 81 K. We demonstrated that the use of a 2-low-temperature spectra method permits one to complete the Ett and J values missed in the previous studies.展开更多
基金supported by the Ministry of Science and Technology of China(2016YFA0501100)the National Natural Science Foundation of China(31825009)to Hong-Wei Wang.
文摘The revolutionary improvement of hardware and algorithm in cryogenic electron microscopy(cryo-EM)has made it a routine method to obtain structures of macromolecules at near-atomic resolution.Nevertheless,this technique still faces many challenges.The structure-solving efficiency of cryo-EM can be significantly reduced by the biomolecules'denaturation on the air–water interfaces,the preferred orientation,strong background noise from supporting films and particle motion,and so forth.To overcome these problems,nanomaterials with ultrahigh electronic conductivity and ultrathin thickness are explored as promising cryo-EM specimen supporting films.Herein,we summarize the structural engineering of graphene,for example,surface and interface modification,as supporting films for grids and the application on high-resolution cryo-EM and discuss potential future perspectives.
基金Project supported by the National Natural Science Foundation of China(Grant No.41175036)
文摘Direct absorption spectra of the 2v3 band of methane （CH4） from 6038 to 6050 cm 1 were studied at different low temperatures using a newly developed cryogenic cell in combination with a distributed feedback （DFB） diode laser. The cryogenic cell can operate at any stabilized temperature ranging from room temperature down to 100 K with temperature fluctuation less than =t=1 K within 1 hour. In the present work, the CH4 spectra in the range of 6038-6050 cm-1 were recorded at 296, 266, 248, 223, 198, and 176 K. The lower state energy Ett and the rotational assignment of the angular momentum J were determined by a ＂2-low-temperature spectra method＂ using the spectra recorded at 198 and 176 K. The results were compared with the data from the GOSAT and the recently reported results from Campargue and co-workers using two spectra measured at room temperature and 81 K. We demonstrated that the use of a 2-low-temperature spectra method permits one to complete the Ett and J values missed in the previous studies.
