In this study,juvenile turbot Scophthalmus maximus were vaccinated with attenuated Edwardsiella tarda(EIBAV1)and reared at two different densities,low density(LD),(5.25±0.02)kg m−2,as control group and high densi...In this study,juvenile turbot Scophthalmus maximus were vaccinated with attenuated Edwardsiella tarda(EIBAV1)and reared at two different densities,low density(LD),(5.25±0.02)kg m−2,as control group and high density(HD),(20.53±0.05)kg m−2,as experimental group.Only density was considered as the variable.Five weeks after vaccination,the transcriptomes of spleen and head kidney from the turbot in two groups were analyzed with RNA-Seq technology.A total of 447 million reads were assembled into 41136 genes with an average length of 1274 bp and a N50 size of 2295 bp.A comparison of gene expression between HD and LD groups revealed 1155 differentially expressed genes(DEGs).Enrichment and pathway analysis of the 10 immune-related DEGs showed the centrality of toll-like receptor signaling pathway,cytosolic DNA-sensing pathway and platelet activation in the host immune responses.The 5 overexpressed inflammatory cytokines and 5 downregulated signal-regulated cytokines genes are covered by these immune-related DEGs.It was inferred that cells suffer damage and the immune response is restrained in turbot under crowding stress.展开更多
基金the National Key R&D Program of China(No.2017YFB0404001)the Central Public-Interest Scientific Institution Basal Research Fund,CAFS(No.2017HYZD04)+1 种基金the Qingdao Shinan District Science and Technology Bureau(No.2016-3-006)the Modern Agriculture Industry System Construction Special Funds(No.CARS-47-G24).
文摘In this study,juvenile turbot Scophthalmus maximus were vaccinated with attenuated Edwardsiella tarda(EIBAV1)and reared at two different densities,low density(LD),(5.25±0.02)kg m−2,as control group and high density(HD),(20.53±0.05)kg m−2,as experimental group.Only density was considered as the variable.Five weeks after vaccination,the transcriptomes of spleen and head kidney from the turbot in two groups were analyzed with RNA-Seq technology.A total of 447 million reads were assembled into 41136 genes with an average length of 1274 bp and a N50 size of 2295 bp.A comparison of gene expression between HD and LD groups revealed 1155 differentially expressed genes(DEGs).Enrichment and pathway analysis of the 10 immune-related DEGs showed the centrality of toll-like receptor signaling pathway,cytosolic DNA-sensing pathway and platelet activation in the host immune responses.The 5 overexpressed inflammatory cytokines and 5 downregulated signal-regulated cytokines genes are covered by these immune-related DEGs.It was inferred that cells suffer damage and the immune response is restrained in turbot under crowding stress.
