Objective: To determine the knowledge, attitude and practice of healthcare workers in Kermanshah Province about Crimean-Congo hemorrhagic fever(CCHF).Methods: This study was conducted in 2014 on healthcare personnel i...Objective: To determine the knowledge, attitude and practice of healthcare workers in Kermanshah Province about Crimean-Congo hemorrhagic fever(CCHF).Methods: This study was conducted in 2014 on healthcare personnel in different job categories including physicians, nurses, midwives, laboratory staff and network health staff of Kermanshah Province by direct interview.Results: A total of 367 respondents who had more than 5 years of experience in their jobs were interviewed. Among them 91% of physicians and nurses, 97% of midwives and health workers and 96% of laboratory staff stated that they had not been confronted with CCHF patients so far. Regarding knowledge, 76% of physicians, 78% of nurses, 77% of midwives and 58% of laboratory staff believed that the disease is remediable. Most of the interviewed participants stated that the disease pertains to people who are in close contact with domestic animals, but they did not consider their own occupations as one of the risk factors. More than 70% of the respondents believed that the disease may exist in the province or their work field. Generally, the knowledge about CCHF was inadequate, with nurses having the lowest level of knowledge.Conclusions: Knowledge of Kermanshah healthcare staff about CCHF was poor,especially nurses in a high risk job category. Therefore, it is necessary to conduct specific training programs for the disease identification, transmission, prevention, and treatment as well as the use of personal protection and safety devices.展开更多
On the basis of sequencing and analyzing of the whole M genes(encoding viral membrane antigen glycoprotein) of three Crimean Congo hemorrhagic fever viruses(CCHFV),the Chinese isolates(Xinjiang hemorrhagic fever virus...On the basis of sequencing and analyzing of the whole M genes(encoding viral membrane antigen glycoprotein) of three Crimean Congo hemorrhagic fever viruses(CCHFV),the Chinese isolates(Xinjiang hemorrhagic fever virus,XHFV),we first expressed the glycoprotein (GP) gene of the prototype human origin XHFV strain BA66019 in eukaryotic cells and investigated the expression profiles.Three eukaryotic expression plasmids were constructed starting from ATG 78 (the first start codon of the deduced entire XHFv GP precursor gene positioned between the 78 th ～80 th nucleotides),ATG 93 and ATG 3084 (the potential start codon for G1 precursor gene).The constructs were transfected to COS-7 cells and the expressed products were characterized as membrane-bound proteins which could induce cell fusion This was much more apparent for recombinant plasmid with ATG 3084 A recombinant baculovirus was further created harboring full length GP gene(starting from ATG 78 ) and the expression could also result in the membrane fusion as well as swelling of the infected Sf9 cells The insect cell expressed G1 was smaller in M W than natural G1 (approximately 67kD) on SDS-PAGE and no recombinant G2 band was detectable Western-blot only detected the native G1,while there was no specific corresponding band of recombinant G1 These data suggested that G1 was structurally and functionally important in XHFV and the glycosylation may have great influence on M W as well as antigenicity This study provides a foundation for the future study of viral pathogenesis ,antigenicity and immunity,that are the theoretical and experimental backgrounds for vaccine展开更多
基金Supported by Kerman University of Medical Sciences,Kerman,Iran(Grant No.94251)
文摘Objective: To determine the knowledge, attitude and practice of healthcare workers in Kermanshah Province about Crimean-Congo hemorrhagic fever(CCHF).Methods: This study was conducted in 2014 on healthcare personnel in different job categories including physicians, nurses, midwives, laboratory staff and network health staff of Kermanshah Province by direct interview.Results: A total of 367 respondents who had more than 5 years of experience in their jobs were interviewed. Among them 91% of physicians and nurses, 97% of midwives and health workers and 96% of laboratory staff stated that they had not been confronted with CCHF patients so far. Regarding knowledge, 76% of physicians, 78% of nurses, 77% of midwives and 58% of laboratory staff believed that the disease is remediable. Most of the interviewed participants stated that the disease pertains to people who are in close contact with domestic animals, but they did not consider their own occupations as one of the risk factors. More than 70% of the respondents believed that the disease may exist in the province or their work field. Generally, the knowledge about CCHF was inadequate, with nurses having the lowest level of knowledge.Conclusions: Knowledge of Kermanshah healthcare staff about CCHF was poor,especially nurses in a high risk job category. Therefore, it is necessary to conduct specific training programs for the disease identification, transmission, prevention, and treatment as well as the use of personal protection and safety devices.
文摘On the basis of sequencing and analyzing of the whole M genes(encoding viral membrane antigen glycoprotein) of three Crimean Congo hemorrhagic fever viruses(CCHFV),the Chinese isolates(Xinjiang hemorrhagic fever virus,XHFV),we first expressed the glycoprotein (GP) gene of the prototype human origin XHFV strain BA66019 in eukaryotic cells and investigated the expression profiles.Three eukaryotic expression plasmids were constructed starting from ATG 78 (the first start codon of the deduced entire XHFv GP precursor gene positioned between the 78 th ～80 th nucleotides),ATG 93 and ATG 3084 (the potential start codon for G1 precursor gene).The constructs were transfected to COS-7 cells and the expressed products were characterized as membrane-bound proteins which could induce cell fusion This was much more apparent for recombinant plasmid with ATG 3084 A recombinant baculovirus was further created harboring full length GP gene(starting from ATG 78 ) and the expression could also result in the membrane fusion as well as swelling of the infected Sf9 cells The insect cell expressed G1 was smaller in M W than natural G1 (approximately 67kD) on SDS-PAGE and no recombinant G2 band was detectable Western-blot only detected the native G1,while there was no specific corresponding band of recombinant G1 These data suggested that G1 was structurally and functionally important in XHFV and the glycosylation may have great influence on M W as well as antigenicity This study provides a foundation for the future study of viral pathogenesis ,antigenicity and immunity,that are the theoretical and experimental backgrounds for vaccine
