The transcription factors FLOWERING LOCUS C(FLC) and SHORT VEGETATIVE PHASE(SVP) can interact to form homologous and heterologous protein complexes that regulate flowering time in Brassica juncea Coss.(Mustard). Previ...The transcription factors FLOWERING LOCUS C(FLC) and SHORT VEGETATIVE PHASE(SVP) can interact to form homologous and heterologous protein complexes that regulate flowering time in Brassica juncea Coss.(Mustard). Previous studies showed that protein interactions were mediated by the K domain,which contains the subdomains K1,K2 and K3. However,it remains unknown how the subdomains mediate the interactions between FLC and SVP. In the present study,we constructed several mutants of subdomains K1–K3 and investigated the mechanisms involved in the heterologous interaction of BjFLC/BjSVP and in the homologous interaction of BjFLC/Bj FLC or BjSVP/BjSVP. Yeast two-hybrid and β-galactosidase activity assays showed that the 19 amino acids of the K1 subdomain in BjSVP and the 17 amino acids of the K1 subdomain in BjFLC were functional subdomains that interact with each other to mediate hetero-dimerization. The heterologous interaction was enhanced by the K2 subdomain of BjSVP protein,but weakened by its interhelical domain L2. The heterologous interaction was also enhanced by the K2 subdomain of BjFLC protein,but weakened by its K3 subdomain. The homologous interaction of BjSVP was mediated by the full K-domain. However,the homologous interaction of BjFLC was regulated only by its K1 and weakened by its K2 and K3 subdomains. The results provided new insights into the interactions between FLC and SVP,which will be valuable for further studies on the molecular regulation mechanisms of the regulation of flowering time in B. juncea and other Brassicaceae.展开更多
以茎瘤芥(Brassica juncea Coss.var.tumida Tsen et Lee)黑斑病病株为试材,对黑斑病菌5.8 S rDNA及其侧翼ITS区序列进行克隆、测序和比对分析。结果表明,5个供试病菌碱基序列同芸薹链格孢的碱基序列相似度达到99.68%,不存在大于3 bp的...以茎瘤芥(Brassica juncea Coss.var.tumida Tsen et Lee)黑斑病病株为试材,对黑斑病菌5.8 S rDNA及其侧翼ITS区序列进行克隆、测序和比对分析。结果表明,5个供试病菌碱基序列同芸薹链格孢的碱基序列相似度达到99.68%,不存在大于3 bp的碱基差异;而与甘蓝链格孢和萝卜链格孢的碱基差异较明显,均存在大于3 bp的碱基差异,且存在大量的缺失片段。初步确定引起茎瘤芥黑斑病的病原菌为芸薹链格孢。展开更多
Objective:To study the bioactivity of methanolic extract of Brassica juncea on animal model of diabetes mellitus along with its effect on diabetic and metabolic parameters.Methods:Diabetes mellitus was induced in rats...Objective:To study the bioactivity of methanolic extract of Brassica juncea on animal model of diabetes mellitus along with its effect on diabetic and metabolic parameters.Methods:Diabetes mellitus was induced in rats by injecting streptozotocin(60 mg/kg)intraperitonealy.Blood glucose was measured on day 3 by GOD-POD method to confirm the diabetes mellitus.Rats having fasting blood glucose>250 mg/d L were further selected for study and they were divided into four groups,control,control+streptozotocin,streptozotocin+metformin(75 mg/kg)and streptozotocin+extract of B.juncea(450 mg/kg).Each group consisted of six rats of either sex.Metformin and experimental extract were administered for 21 d.Triglyceride,cholesterol level were measured on day 21 by commercially available kit.Blood glucose was measured on days 7 and 21.Anti-oxidant potential was assessed by estimating extent of lipid peroxidation(LPO)by malondialdehyde(MDA),nitric oxide(NO),superoxide dismutase(SOD)and glutathione(GSH)in liver,kidney,pancreas,muscle tissues on day 21.Unpaired and paired student’s t-test was applied for statistical analysis.Results:The extract of B.juncea showed significant decrease in blood glucose level on day 21.The treatment group showed significant difference in oxidative stress by increasing SOD and GSH and decreasing LPO and NO activity on day 21.The treatment did not show statistically significant difference of cholesterol,and triglycerides level on day 21.Conclusion:The study showed anti-hyperglycemic and anti-oxidative properties of methanolic extract of B.juncea.展开更多
The rich genetic variation preserved in collections of Aegilops tauschii can be readily exploited to improve common wheat using synthetic hexaploid wheat lines. However,hybrid necrosis, which is characterized by progr...The rich genetic variation preserved in collections of Aegilops tauschii can be readily exploited to improve common wheat using synthetic hexaploid wheat lines. However,hybrid necrosis, which is characterized by progressive death of leaves or plants, has been observed in certain interspecific crosses between tetraploid wheat and Ae. tauschii. The aim of this study was to construct a fine genetic map of a gene(temporarily named Net Jing Y176)conferring hybrid necrosis in Ae. tauschii accession Jing Y176. A triploid F1 population derived from distant hybridization between Ae. tauschii and tetraploid wheat was used to map the gene with microsatellite markers. The newly developed markers Xsdau K539 and Xsdau K561 co-segregated with Net Jing Y176 on chromosome arm 2DS. The tightly linked markers developed in this study were used to genotype 91 Ae. tauschii accessions. The marker genotype analysis suggested that 49.45% of the Ae. tauschii accessions carry Net Jing Y176. Interestingly, hybrid necrosis genotypes tended to appear more commonly in Ae. tauschii ssp. tauschii than in Ae. tauschii ssp. strangulata.展开更多
The present study depicts the therapeutic effect of Zygophyllum cornutum methanolic extract （ZCME） on metabolic disturbances, oxidative stress in heart and myocardium histological changes in streptozotocin （STZ）-i...The present study depicts the therapeutic effect of Zygophyllum cornutum methanolic extract （ZCME） on metabolic disturbances, oxidative stress in heart and myocardium histological changes in streptozotocin （STZ）-induced diabetic rats. Three days after diabetes induction, ZCME was administered orally for six weeks （700 mg/kg bw/day）. Serum glucose and lipid profile were evaluated. Reduced glutathione （GSH）, catalase （CAT） and thiobarbituric acid reactive substances （TBARS） were measured in heart tissue. The results showed increased levels of blood glucose, total cholesterol （TC）, LDL cholesterol （LDL-C） and triglyceride （TG） in the diabetic rats. On the other hand, the level of HDL cholesterol （HDL-C） decreased. Compared to the control normal rats, the level of TBARS in heart tissue was markedly increased while GSH and CAT were significantly modified in the diabetic rats. Oral administration of ZCME has normalized serum glucose and lipid profile. TBARS were significantly reduced in heart while CAT and GSH were markedly normalized. Myocardium sections showed the absence of histological changes observed in the diabetic rats. The study suggests that Zygophyllum cornutum may provide a useful therapeutic option in the reversal of metabolic disturbances and oxidative stress-induced cardiac dysfunction in diabetes mellitus.展开更多
Root and shoot growth of Brassica campestris L. and B. juncea increased significantly （P 〈 0.01） with enhanced Ca2＋ treatment along with 60 mM NaCl in the root medium. The maximum fresh mass of shoot and root in B...Root and shoot growth of Brassica campestris L. and B. juncea increased significantly （P 〈 0.01） with enhanced Ca2＋ treatment along with 60 mM NaCl in the root medium. The maximum fresh mass of shoot and root in B.juncea was recorded at 10 mM Ca2＋ concentration. The relative growth rate of shoot of both species reached its maximum at 8 mM of Ca2＋ concentration. Average rate of Ca2＋ intake （lCa） was higher in B. juncea than 6. campestris. In B. juncea, the average transport of Ca2＋ to shoot increased by 19%, 38%, 119%, 125% and 169% compared with the control. Furthermore specific utilization rate of Ca2＋ was higher in B. juncea than B. campestris. In B. campestris it increased by 9%, 32%, 41% and 59% at 4, 6, 8, and 10 mM of calcium in comparison to 2 mM Ca2＋ treatment. At 4, 6, 8 and 10 mM of Ca2＋ application, the increase in the leaf area ratio was 10,17, 23 and 30%, respectively. In the shoot and root portions of B. campestris and B.juncea, Ca2＋ had a linear relationship with potassium and sulfur, whereas it was in antagonism with sodium ion.展开更多
基金supported financially by grants from the National Program on Key Basic Research Projects(The 973 Program:2012CB113900)the National Natural Science Foundation of China(31000908)+1 种基金the Chongqing Natural Science Foundation (2011BA1002)the Fundamental Research Funds for the Central Universities (XDJK2012B020)
文摘The transcription factors FLOWERING LOCUS C(FLC) and SHORT VEGETATIVE PHASE(SVP) can interact to form homologous and heterologous protein complexes that regulate flowering time in Brassica juncea Coss.(Mustard). Previous studies showed that protein interactions were mediated by the K domain,which contains the subdomains K1,K2 and K3. However,it remains unknown how the subdomains mediate the interactions between FLC and SVP. In the present study,we constructed several mutants of subdomains K1–K3 and investigated the mechanisms involved in the heterologous interaction of BjFLC/BjSVP and in the homologous interaction of BjFLC/Bj FLC or BjSVP/BjSVP. Yeast two-hybrid and β-galactosidase activity assays showed that the 19 amino acids of the K1 subdomain in BjSVP and the 17 amino acids of the K1 subdomain in BjFLC were functional subdomains that interact with each other to mediate hetero-dimerization. The heterologous interaction was enhanced by the K2 subdomain of BjSVP protein,but weakened by its interhelical domain L2. The heterologous interaction was also enhanced by the K2 subdomain of BjFLC protein,but weakened by its K3 subdomain. The homologous interaction of BjSVP was mediated by the full K-domain. However,the homologous interaction of BjFLC was regulated only by its K1 and weakened by its K2 and K3 subdomains. The results provided new insights into the interactions between FLC and SVP,which will be valuable for further studies on the molecular regulation mechanisms of the regulation of flowering time in B. juncea and other Brassicaceae.
文摘以茎瘤芥(Brassica juncea Coss.var.tumida Tsen et Lee)黑斑病病株为试材,对黑斑病菌5.8 S rDNA及其侧翼ITS区序列进行克隆、测序和比对分析。结果表明,5个供试病菌碱基序列同芸薹链格孢的碱基序列相似度达到99.68%,不存在大于3 bp的碱基差异;而与甘蓝链格孢和萝卜链格孢的碱基差异较明显,均存在大于3 bp的碱基差异,且存在大量的缺失片段。初步确定引起茎瘤芥黑斑病的病原菌为芸薹链格孢。
基金Banaras Hindu University(BHU)for providing grant to carry out study.
文摘Objective:To study the bioactivity of methanolic extract of Brassica juncea on animal model of diabetes mellitus along with its effect on diabetic and metabolic parameters.Methods:Diabetes mellitus was induced in rats by injecting streptozotocin(60 mg/kg)intraperitonealy.Blood glucose was measured on day 3 by GOD-POD method to confirm the diabetes mellitus.Rats having fasting blood glucose>250 mg/d L were further selected for study and they were divided into four groups,control,control+streptozotocin,streptozotocin+metformin(75 mg/kg)and streptozotocin+extract of B.juncea(450 mg/kg).Each group consisted of six rats of either sex.Metformin and experimental extract were administered for 21 d.Triglyceride,cholesterol level were measured on day 21 by commercially available kit.Blood glucose was measured on days 7 and 21.Anti-oxidant potential was assessed by estimating extent of lipid peroxidation(LPO)by malondialdehyde(MDA),nitric oxide(NO),superoxide dismutase(SOD)and glutathione(GSH)in liver,kidney,pancreas,muscle tissues on day 21.Unpaired and paired student’s t-test was applied for statistical analysis.Results:The extract of B.juncea showed significant decrease in blood glucose level on day 21.The treatment group showed significant difference in oxidative stress by increasing SOD and GSH and decreasing LPO and NO activity on day 21.The treatment did not show statistically significant difference of cholesterol,and triglycerides level on day 21.Conclusion:The study showed anti-hyperglycemic and anti-oxidative properties of methanolic extract of B.juncea.
基金financially supported by the National Natural Science Foundation of China (grant numbers 31171553 and 31471488)the National Basic Research Program of China (2014CB138100)+2 种基金the National High Technology Research and Development Program of China (2011AA100102 and 2012AA101105)Shandong Seed Engineering Project (2015–2019)the Program of Introducing International Super Agricultural Science and Technology (948 program, 2013-S19)
文摘The rich genetic variation preserved in collections of Aegilops tauschii can be readily exploited to improve common wheat using synthetic hexaploid wheat lines. However,hybrid necrosis, which is characterized by progressive death of leaves or plants, has been observed in certain interspecific crosses between tetraploid wheat and Ae. tauschii. The aim of this study was to construct a fine genetic map of a gene(temporarily named Net Jing Y176)conferring hybrid necrosis in Ae. tauschii accession Jing Y176. A triploid F1 population derived from distant hybridization between Ae. tauschii and tetraploid wheat was used to map the gene with microsatellite markers. The newly developed markers Xsdau K539 and Xsdau K561 co-segregated with Net Jing Y176 on chromosome arm 2DS. The tightly linked markers developed in this study were used to genotype 91 Ae. tauschii accessions. The marker genotype analysis suggested that 49.45% of the Ae. tauschii accessions carry Net Jing Y176. Interestingly, hybrid necrosis genotypes tended to appear more commonly in Ae. tauschii ssp. tauschii than in Ae. tauschii ssp. strangulata.
文摘The present study depicts the therapeutic effect of Zygophyllum cornutum methanolic extract （ZCME） on metabolic disturbances, oxidative stress in heart and myocardium histological changes in streptozotocin （STZ）-induced diabetic rats. Three days after diabetes induction, ZCME was administered orally for six weeks （700 mg/kg bw/day）. Serum glucose and lipid profile were evaluated. Reduced glutathione （GSH）, catalase （CAT） and thiobarbituric acid reactive substances （TBARS） were measured in heart tissue. The results showed increased levels of blood glucose, total cholesterol （TC）, LDL cholesterol （LDL-C） and triglyceride （TG） in the diabetic rats. On the other hand, the level of HDL cholesterol （HDL-C） decreased. Compared to the control normal rats, the level of TBARS in heart tissue was markedly increased while GSH and CAT were significantly modified in the diabetic rats. Oral administration of ZCME has normalized serum glucose and lipid profile. TBARS were significantly reduced in heart while CAT and GSH were markedly normalized. Myocardium sections showed the absence of histological changes observed in the diabetic rats. The study suggests that Zygophyllum cornutum may provide a useful therapeutic option in the reversal of metabolic disturbances and oxidative stress-induced cardiac dysfunction in diabetes mellitus.
文摘Root and shoot growth of Brassica campestris L. and B. juncea increased significantly （P 〈 0.01） with enhanced Ca2＋ treatment along with 60 mM NaCl in the root medium. The maximum fresh mass of shoot and root in B.juncea was recorded at 10 mM Ca2＋ concentration. The relative growth rate of shoot of both species reached its maximum at 8 mM of Ca2＋ concentration. Average rate of Ca2＋ intake （lCa） was higher in B. juncea than 6. campestris. In B. juncea, the average transport of Ca2＋ to shoot increased by 19%, 38%, 119%, 125% and 169% compared with the control. Furthermore specific utilization rate of Ca2＋ was higher in B. juncea than B. campestris. In B. campestris it increased by 9%, 32%, 41% and 59% at 4, 6, 8, and 10 mM of calcium in comparison to 2 mM Ca2＋ treatment. At 4, 6, 8 and 10 mM of Ca2＋ application, the increase in the leaf area ratio was 10,17, 23 and 30%, respectively. In the shoot and root portions of B. campestris and B.juncea, Ca2＋ had a linear relationship with potassium and sulfur, whereas it was in antagonism with sodium ion.
