Salmonella Corvallis ST1541 has recently emerged as a globally disseminated pathogenic strain that often causes severe food-borne infections.Unlike most pandemic serotypes of Salmonella,the ST1541 strains harbored Col...Salmonella Corvallis ST1541 has recently emerged as a globally disseminated pathogenic strain that often causes severe food-borne infections.Unlike most pandemic serotypes of Salmonella,the ST1541 strains harbored ColRNA1 plasmids that contain qnr-like determinants known to be responsible for the increasing incidence of ciprofloxacin-resistant food-borne Salmonella infections.In this study,we conducted a genomic analysis of a global collection of 388 S.Corvallis ST1541 strains collected within a twenty-year period.We investigated the genetic characteristics of plasmid-mediated quinolone resistance(PMQR)plasmids harbored by these S.Corvallis strains,established a mini-mum spanning tree(MST)to determine the temporal and spatial distribution of the top 10 MST clusters,inferred a time-phylogenies for the major sub-lineages and traced the routes of international dissemination of this serotype strains.Bayesian algorithm predicted that UK might be the origin of S.Corvallis strains currently prevalent in various countries.This idea is supported by the observation of the emergence of intercontinental-disseminated clonal strains and extensive transmission of the extensive-drug resistance(XDR)-encoding plasmid pSA663.This study there-fore provides valuable insight into the evolution of globally transmitted S.Corvallis strains and suggests a need to strengthen cooperation between different countries to control the dissemination of these drug-resistant bacteria.展开更多
目的了解2016年河南省食源性沙门菌优势血清型(科瓦利斯沙门菌)药物敏感性和分子分型特征。方法食品中沙门菌的检测和血清分型参照2016年国家食品污染物和有害因素风险监测工作手册、脉冲场凝胶电泳(pulsed field gel electrophoresis, ...目的了解2016年河南省食源性沙门菌优势血清型(科瓦利斯沙门菌)药物敏感性和分子分型特征。方法食品中沙门菌的检测和血清分型参照2016年国家食品污染物和有害因素风险监测工作手册、脉冲场凝胶电泳(pulsed field gel electrophoresis, PFGE)分子分型分析和药物敏感试验参照2016年食源性疾病监测工作手册和国家食源性疾病分子溯源网络(TraNetChina)使用手册。结果采用微量最低抑菌浓度法对20株食源性科瓦利斯沙门菌进行15种抗生素的药物敏感试验,20株菌对四环素、红霉素100%耐药,对环丙沙星、萘啶酸耐药率分别为80.00%、45.00%,对头孢西丁、头孢唑啉产生中度耐药,中度耐药率分别为15.00%、10.00%,对头孢噻圬、头孢他啶、亚胺培南、庆大霉素、氨苄西林、氨苄西林/舒巴坦、氯霉素、甲氧苄氨嘧啶/磺胺甲噁唑、阿奇霉素全部敏感。有8株菌出现多重耐药现象,最多对4类4种抗生素产生耐药。20株科瓦利斯沙门菌用XbaⅠ酶切、经脉冲场凝胶电泳后,获得9种带型,每种带型包括1～6株菌,相似度61.7%～100%,以SCVX01.HA0008和SCVX01.HA0009为主要优势带型。结论河南省食源性科瓦利斯沙门菌对第三代头孢类抗生素全部敏感,对喹诺酮类和氟喹诺酮类出现不同程度耐药,且带型存在地区聚集性提示存在共同来源或者交叉污染的情况,相关部门应加强监测,提早防范。展开更多
基金supported by the Key Research and Development Program of Guangdong Province(Grant number,2022B1111040002)the Collaborative Research Fund from Research Grant Council of the Government of Hong Kong SAR(Grant numbers,C7147-20G,C7003-20G).
文摘Salmonella Corvallis ST1541 has recently emerged as a globally disseminated pathogenic strain that often causes severe food-borne infections.Unlike most pandemic serotypes of Salmonella,the ST1541 strains harbored ColRNA1 plasmids that contain qnr-like determinants known to be responsible for the increasing incidence of ciprofloxacin-resistant food-borne Salmonella infections.In this study,we conducted a genomic analysis of a global collection of 388 S.Corvallis ST1541 strains collected within a twenty-year period.We investigated the genetic characteristics of plasmid-mediated quinolone resistance(PMQR)plasmids harbored by these S.Corvallis strains,established a mini-mum spanning tree(MST)to determine the temporal and spatial distribution of the top 10 MST clusters,inferred a time-phylogenies for the major sub-lineages and traced the routes of international dissemination of this serotype strains.Bayesian algorithm predicted that UK might be the origin of S.Corvallis strains currently prevalent in various countries.This idea is supported by the observation of the emergence of intercontinental-disseminated clonal strains and extensive transmission of the extensive-drug resistance(XDR)-encoding plasmid pSA663.This study there-fore provides valuable insight into the evolution of globally transmitted S.Corvallis strains and suggests a need to strengthen cooperation between different countries to control the dissemination of these drug-resistant bacteria.
文摘目的了解2016年河南省食源性沙门菌优势血清型(科瓦利斯沙门菌)药物敏感性和分子分型特征。方法食品中沙门菌的检测和血清分型参照2016年国家食品污染物和有害因素风险监测工作手册、脉冲场凝胶电泳(pulsed field gel electrophoresis, PFGE)分子分型分析和药物敏感试验参照2016年食源性疾病监测工作手册和国家食源性疾病分子溯源网络(TraNetChina)使用手册。结果采用微量最低抑菌浓度法对20株食源性科瓦利斯沙门菌进行15种抗生素的药物敏感试验,20株菌对四环素、红霉素100%耐药,对环丙沙星、萘啶酸耐药率分别为80.00%、45.00%,对头孢西丁、头孢唑啉产生中度耐药,中度耐药率分别为15.00%、10.00%,对头孢噻圬、头孢他啶、亚胺培南、庆大霉素、氨苄西林、氨苄西林/舒巴坦、氯霉素、甲氧苄氨嘧啶/磺胺甲噁唑、阿奇霉素全部敏感。有8株菌出现多重耐药现象,最多对4类4种抗生素产生耐药。20株科瓦利斯沙门菌用XbaⅠ酶切、经脉冲场凝胶电泳后,获得9种带型,每种带型包括1～6株菌,相似度61.7%～100%,以SCVX01.HA0008和SCVX01.HA0009为主要优势带型。结论河南省食源性科瓦利斯沙门菌对第三代头孢类抗生素全部敏感,对喹诺酮类和氟喹诺酮类出现不同程度耐药,且带型存在地区聚集性提示存在共同来源或者交叉污染的情况,相关部门应加强监测,提早防范。
