With the enhancement of copper （Cu） stress, the germination percentage of wheat seeds decreased gradually. Pretreatment with sodium hydrosulfide （NariS）, hydrogen sulfide （H2S） donor alleviated the inhibitory ef...With the enhancement of copper （Cu） stress, the germination percentage of wheat seeds decreased gradually. Pretreatment with sodium hydrosulfide （NariS）, hydrogen sulfide （H2S） donor alleviated the inhibitory effect of Cu stress in a dose- dependent manner; whereas little visible symptom was observed in germinating seeds and radicle tips cultured in NariS solutions. It was verified that H2S or HS- rather than other sulfur-containing components derived from NariS attribute to the potential role in promoting seed germination against Cu stress. Further studies showed that NariS could promote amylase and esterase activities, reduce Cu-induced disturbance of plasma membrane integrity in the radicle tips, and sustain lower levels of malondialdehyde and H202 in germinating seeds. Furthermore, NariS pretreatment increased activities of superoxide dismutase and catalase and decreased that of lipoxygenase, but showed no significant effect on ascorbate peroxidase. Alternatively, NariS prevented uptake of Cu and promoted the accumulation of free amino acids in seeds exposed to Cu. In addition, a rapid accumulation of endogenous H2S in seeds was observed at the early stage of germination, and higher level of H2S in NaHS-pretreated seeds. These data indicated that H2S was involved in the mechanism of germinating seeds＇ responses to Cu stress.展开更多
采用AG MP-1阴离子交换树脂,分别以7 mol/L HC l、2 mol/L HC l、0.5 mol/L HNO3作为淋洗剂,可有效分离Cu、Fe、Zn。介绍了方法的基本原理、化学分离过程及混合标准溶液与地质标样的分离结果。结果表明,Cu、Fe、Zn回收率均接近100%,标...采用AG MP-1阴离子交换树脂,分别以7 mol/L HC l、2 mol/L HC l、0.5 mol/L HNO3作为淋洗剂,可有效分离Cu、Fe、Zn。介绍了方法的基本原理、化学分离过程及混合标准溶液与地质标样的分离结果。结果表明,Cu、Fe、Zn回收率均接近100%,标准溶液在离子交换分离前后同位素组成一致,可以满足多接收器等离子体质谱对Cu、Fe、Zn同位素高精度分析的要求。展开更多
基金Supported by the Natural Science Foundation of Anhui Province (070411009)the Innovation Fund from Hefei University of Technology to undergraduate students (XS0637).
文摘With the enhancement of copper （Cu） stress, the germination percentage of wheat seeds decreased gradually. Pretreatment with sodium hydrosulfide （NariS）, hydrogen sulfide （H2S） donor alleviated the inhibitory effect of Cu stress in a dose- dependent manner; whereas little visible symptom was observed in germinating seeds and radicle tips cultured in NariS solutions. It was verified that H2S or HS- rather than other sulfur-containing components derived from NariS attribute to the potential role in promoting seed germination against Cu stress. Further studies showed that NariS could promote amylase and esterase activities, reduce Cu-induced disturbance of plasma membrane integrity in the radicle tips, and sustain lower levels of malondialdehyde and H202 in germinating seeds. Furthermore, NariS pretreatment increased activities of superoxide dismutase and catalase and decreased that of lipoxygenase, but showed no significant effect on ascorbate peroxidase. Alternatively, NariS prevented uptake of Cu and promoted the accumulation of free amino acids in seeds exposed to Cu. In addition, a rapid accumulation of endogenous H2S in seeds was observed at the early stage of germination, and higher level of H2S in NaHS-pretreated seeds. These data indicated that H2S was involved in the mechanism of germinating seeds＇ responses to Cu stress.
文摘采用AG MP-1阴离子交换树脂,分别以7 mol/L HC l、2 mol/L HC l、0.5 mol/L HNO3作为淋洗剂,可有效分离Cu、Fe、Zn。介绍了方法的基本原理、化学分离过程及混合标准溶液与地质标样的分离结果。结果表明,Cu、Fe、Zn回收率均接近100%,标准溶液在离子交换分离前后同位素组成一致,可以满足多接收器等离子体质谱对Cu、Fe、Zn同位素高精度分析的要求。
