植物为了维持其生命系统的正常运转,需要对各种代谢产物和毒素进行转运和排出。多药与毒性化合物排出转运蛋白(multidrug and toxic compound extrusion,MATE)在多种底物和毒素的运输中起到重要作用。本研究利用生物信息学手段对芝麻MAT...植物为了维持其生命系统的正常运转,需要对各种代谢产物和毒素进行转运和排出。多药与毒性化合物排出转运蛋白(multidrug and toxic compound extrusion,MATE)在多种底物和毒素的运输中起到重要作用。本研究利用生物信息学手段对芝麻MATE基因家族进行了全基因组分析,鉴定得到67个MATE基因,分布于全部13条染色体上,亚细胞定位预测表明这些基因主要位于质膜上。串联复制和全基因组复制是芝麻MATE基因家族扩增的主要动力。比较基因组学分析发现在芝麻和拟南芥中具有许多共线性的MATE基因,且大部分串联复制SiMATE基因产生于芝麻和拟南芥分化之后。系统进化分析可将芝麻MATE成员分为4个亚家族,大部分相似功能的已知植物MATE成员被聚在同一分枝中,进化树中关系较近的芝麻MATE成员往往具有相似的基因结构和保守基序。基因表达分析表明一半以上的SiMATE基因具有组织表达特异性。这些结果为芝麻MATE基因功能的研究提供了重要参考。展开更多
A novel coronavirus has been identified as the causative agent of the severe acute respiratory syndrome(SARS). For all the SARS-CoV associated proteins derivatedfrom the SARS-CoV genome, the physiochemical propertiess...A novel coronavirus has been identified as the causative agent of the severe acute respiratory syndrome(SARS). For all the SARS-CoV associated proteins derivatedfrom the SARS-CoV genome, the physiochemical propertiessuch as the molecular weight, isoelectric point and extinction coefficient of each protein were calculated. Thetransmembrane segments and subcellular localization(SubLocation) prediction and conserved protein motifssearch against database were employed to analyze thefunction of SARS-CoV proteins. Also, the homology protein sequence alignment and evolutionary distance matrixcalculation between SARS-CoV associated proteins and the corresponding proteins of other coronaviruses wereemployed to identify the classification and phylogeneticrelationship between SARS-CoV and other coronaviruses.The results showed that SARS-CoV is a novel coronavirus which is different from any of the three previously knowngroups of coronviruses, but it is closer to BoCoV and MHV than to other coronaviruses. This study is in aid ofexperimental determination of SARS-CoV proteomics andthe development of antiviral vaccine.展开更多
文摘植物为了维持其生命系统的正常运转,需要对各种代谢产物和毒素进行转运和排出。多药与毒性化合物排出转运蛋白(multidrug and toxic compound extrusion,MATE)在多种底物和毒素的运输中起到重要作用。本研究利用生物信息学手段对芝麻MATE基因家族进行了全基因组分析,鉴定得到67个MATE基因,分布于全部13条染色体上,亚细胞定位预测表明这些基因主要位于质膜上。串联复制和全基因组复制是芝麻MATE基因家族扩增的主要动力。比较基因组学分析发现在芝麻和拟南芥中具有许多共线性的MATE基因,且大部分串联复制SiMATE基因产生于芝麻和拟南芥分化之后。系统进化分析可将芝麻MATE成员分为4个亚家族,大部分相似功能的已知植物MATE成员被聚在同一分枝中,进化树中关系较近的芝麻MATE成员往往具有相似的基因结构和保守基序。基因表达分析表明一半以上的SiMATE基因具有组织表达特异性。这些结果为芝麻MATE基因功能的研究提供了重要参考。
基金supported by the Natural Science Foundation of Heilongjiang ProvinceChina (No. C200624)+4 种基金the Scientific Research Fund of Department of EducationHeilongjiang ProvinceChina (No. 1521307)the Program for Young Teachers Scientific Research in Qiqihar UniversityChina (No. 2010K-Z10)
文摘A novel coronavirus has been identified as the causative agent of the severe acute respiratory syndrome(SARS). For all the SARS-CoV associated proteins derivatedfrom the SARS-CoV genome, the physiochemical propertiessuch as the molecular weight, isoelectric point and extinction coefficient of each protein were calculated. Thetransmembrane segments and subcellular localization(SubLocation) prediction and conserved protein motifssearch against database were employed to analyze thefunction of SARS-CoV proteins. Also, the homology protein sequence alignment and evolutionary distance matrixcalculation between SARS-CoV associated proteins and the corresponding proteins of other coronaviruses wereemployed to identify the classification and phylogeneticrelationship between SARS-CoV and other coronaviruses.The results showed that SARS-CoV is a novel coronavirus which is different from any of the three previously knowngroups of coronviruses, but it is closer to BoCoV and MHV than to other coronaviruses. This study is in aid ofexperimental determination of SARS-CoV proteomics andthe development of antiviral vaccine.
