目的探讨缝隙连接(GJ)在脑缺血再灌注血脑屏障通透性变化中的作用及其可能机制。方法①应用激光共聚焦显微镜技术观察 GJ 蛋白 Cx43在缺血再灌注半暗带区脑毛细血管周终足上含量及分布情况的变化。②将 Wistar 大鼠随机分成假手术组,手...目的探讨缝隙连接(GJ)在脑缺血再灌注血脑屏障通透性变化中的作用及其可能机制。方法①应用激光共聚焦显微镜技术观察 GJ 蛋白 Cx43在缺血再灌注半暗带区脑毛细血管周终足上含量及分布情况的变化。②将 Wistar 大鼠随机分成假手术组,手术组。线栓法制备大鼠大脑中动脉缺血再灌注模型。通过荧光分光光度定量的方法测定不同时间点脑组织中的伊文蓝含量来观察血脑屏障的通透性的改变。③取伊文蓝漏出最多的时间点,增设辛醇干预组和 DMSO 溶剂对照组,与相同时间点手术对照组比较,观察辛醇对血脑屏障通透性的影响。结果缺血2 h 再灌注3 h 脑组织伊文蓝含量开始增加,再灌注24 h 达高峰。激光共聚焦显微镜发现 GJ 蛋白 Cx43在脑内毛细血管周围的星形胶质细胞终足上分布密集。在缺血2 h 再灌注24 h 半暗带内,终足上的 Cx43分布发生变化,聚集成较大斑块。在缺血2 h 再灌注24 h 组术前给予辛醇干预,脑组织伊文蓝含量[(4.924±0.296)μg/g]低于手术对照组[(5.543±0.506)μg/g],二者差异有统计学意义(P<0.05)。结论GJ 在脑缺血再灌注后半暗带终足上分布变化明显,可能加重了血脑屏障通透性的增加;辛醇阻断 GJ可以降低脑缺血再灌注血脑屏障的通透性,从而起到减轻脑水肿的作用。展开更多
Gap junctions are intercellular channels, which connect adjacent cells and allow direct molecular exchange of low molecular weight between them. Three protein families including connexins, innexins and ponnexins are i...Gap junctions are intercellular channels, which connect adjacent cells and allow direct molecular exchange of low molecular weight between them. Three protein families including connexins, innexins and ponnexins are involved in gap junctions. Connexins are only found in chordate and innexins compose the gap junctions of invertebrates, while pannexins are ubiquitous and present in both vertebrates and invertebrates. Gap junctions in metazoan play important roles in electrical coupling, regulating proliferation, differentiation, and embryonic development. Evidently, the defects of gap junctions resulting form proteins contribute to many diseases of vertebrates and invertebrates, such as tumor, heart diseases, nervous system disorders and other diseases in human.展开更多
文摘目的探讨缝隙连接(GJ)在脑缺血再灌注血脑屏障通透性变化中的作用及其可能机制。方法①应用激光共聚焦显微镜技术观察 GJ 蛋白 Cx43在缺血再灌注半暗带区脑毛细血管周终足上含量及分布情况的变化。②将 Wistar 大鼠随机分成假手术组,手术组。线栓法制备大鼠大脑中动脉缺血再灌注模型。通过荧光分光光度定量的方法测定不同时间点脑组织中的伊文蓝含量来观察血脑屏障的通透性的改变。③取伊文蓝漏出最多的时间点,增设辛醇干预组和 DMSO 溶剂对照组,与相同时间点手术对照组比较,观察辛醇对血脑屏障通透性的影响。结果缺血2 h 再灌注3 h 脑组织伊文蓝含量开始增加,再灌注24 h 达高峰。激光共聚焦显微镜发现 GJ 蛋白 Cx43在脑内毛细血管周围的星形胶质细胞终足上分布密集。在缺血2 h 再灌注24 h 半暗带内,终足上的 Cx43分布发生变化,聚集成较大斑块。在缺血2 h 再灌注24 h 组术前给予辛醇干预,脑组织伊文蓝含量[(4.924±0.296)μg/g]低于手术对照组[(5.543±0.506)μg/g],二者差异有统计学意义(P<0.05)。结论GJ 在脑缺血再灌注后半暗带终足上分布变化明显,可能加重了血脑屏障通透性的增加;辛醇阻断 GJ可以降低脑缺血再灌注血脑屏障的通透性,从而起到减轻脑水肿的作用。
文摘Gap junctions are intercellular channels, which connect adjacent cells and allow direct molecular exchange of low molecular weight between them. Three protein families including connexins, innexins and ponnexins are involved in gap junctions. Connexins are only found in chordate and innexins compose the gap junctions of invertebrates, while pannexins are ubiquitous and present in both vertebrates and invertebrates. Gap junctions in metazoan play important roles in electrical coupling, regulating proliferation, differentiation, and embryonic development. Evidently, the defects of gap junctions resulting form proteins contribute to many diseases of vertebrates and invertebrates, such as tumor, heart diseases, nervous system disorders and other diseases in human.
