Cholate-CoA ligase （,EEL） and bile acid-CoA： amino acid N-acyltransferase （BAAT） sequentially mediate bile-acid amidation. Defects can cause intrahepatic cholestasis. Distinction has required gene sequencing. We ...Cholate-CoA ligase （,EEL） and bile acid-CoA： amino acid N-acyltransferase （BAAT） sequentially mediate bile-acid amidation. Defects can cause intrahepatic cholestasis. Distinction has required gene sequencing. We assessed potential clinical utility of immunostaining of liver for CCL and BAAT. Using commercially available antibodies against BAAT and CCL, we immunostained liver from an infant with jaundice, deficiency of amidated bile acids, and transcription-terminating mutation in BAAT. CCL was normally expressed. BAAT expression was not de- tected. Immunostaining may facilitate diagnosis in bile- acid amidation defects.展开更多
Focusing light though scattering media beyond the ballistic regime is a challenging task in biomedical optical imaging.This challenge can be overcome by wavefront shaping technique,in which a time reversed(TR)wavefron...Focusing light though scattering media beyond the ballistic regime is a challenging task in biomedical optical imaging.This challenge can be overcome by wavefront shaping technique,in which a time reversed(TR)wavefront of scattered light is generated to suppress the scattering.In previous TR optical focusing experiments,a phase only spatial light modulator(SLM)has.been typically used to control the wavefront of incident light.Unfortunately,although the phase information is reconstructed by the phase-only SLM,the amplitude information is lost,resulting in decreased peak to-background ratio(PBR)of optical focusing in the TR wavefront recon-struction.A new method of TR optical focusing through scattering media is proposed here,which numerically reonstructs the full phase and amplitude of a simulated scattered light field by using a single phase only SLM.Simulation results and the proposed optical setup show that the time-reversal of a fully developed speckle field can be digially implemented with both phase and ampltude recovery,affording a way to improve the performance of light focusing through scattering media.展开更多
基金Supported by Great Ormond Street Hospital Children’s Charity, to Clayton PTNational Institutes of HealthGrant R01 DK58214, to Bull LN
文摘Cholate-CoA ligase （,EEL） and bile acid-CoA： amino acid N-acyltransferase （BAAT） sequentially mediate bile-acid amidation. Defects can cause intrahepatic cholestasis. Distinction has required gene sequencing. We assessed potential clinical utility of immunostaining of liver for CCL and BAAT. Using commercially available antibodies against BAAT and CCL, we immunostained liver from an infant with jaundice, deficiency of amidated bile acids, and transcription-terminating mutation in BAAT. CCL was normally expressed. BAAT expression was not de- tected. Immunostaining may facilitate diagnosis in bile- acid amidation defects.
基金supported by the National Science Foundation of China (61177018)the Program for New Century Excellent Talents in University (NECT-11-0596)Beijing Nova Program (2011066).
文摘Focusing light though scattering media beyond the ballistic regime is a challenging task in biomedical optical imaging.This challenge can be overcome by wavefront shaping technique,in which a time reversed(TR)wavefront of scattered light is generated to suppress the scattering.In previous TR optical focusing experiments,a phase only spatial light modulator(SLM)has.been typically used to control the wavefront of incident light.Unfortunately,although the phase information is reconstructed by the phase-only SLM,the amplitude information is lost,resulting in decreased peak to-background ratio(PBR)of optical focusing in the TR wavefront recon-struction.A new method of TR optical focusing through scattering media is proposed here,which numerically reonstructs the full phase and amplitude of a simulated scattered light field by using a single phase only SLM.Simulation results and the proposed optical setup show that the time-reversal of a fully developed speckle field can be digially implemented with both phase and ampltude recovery,affording a way to improve the performance of light focusing through scattering media.
