A full-length genome of a Chinese porcine reproductive and respiratory syndrome virus (PRRSV) isolate and designated as HB-1(sh)/2002,was sequenced and analyzed. The size of complete genome for the isolate is 15,411 n...A full-length genome of a Chinese porcine reproductive and respiratory syndrome virus (PRRSV) isolate and designated as HB-1(sh)/2002,was sequenced and analyzed. The size of complete genome for the isolate is 15,411 nucleotides obtained from a set of overlapping cDNA clones excluding poly A tail,and is comprised of 9 ORFs,two of which encode nonstructural proteins and the others encode the structural proteins. The full-length sequence of HB-1(sh)/2002 was compared with that of two Chinese isolates,BJ-4 and CH-1a.Comparative analysis presented that the complete nucleotide sequence of HB-1(sh)/2002 shared 89.8% and 96.0% identity with BJ-4 and CH-1a,respectively.Compared with the other isolates,it seemed to be more extensive in Nsp2 of nonstructural regions and ORF5 of structural coding regions,respectively.Especially,Nsp2 only shared 77.3% and 89.1% identity with BJ-4 and CH-1a.The ORF5 shared 87.0% and 93.5% identity with BJ-4 and CH-1a.Phylogenetic analysis based on Nsp2 deduced amino acid sequence suggested that HB-1(sh)/2002 belongs to the same subgroup as CH-1a, and Nsp2 can be used as basis for monitoring the genetic variation and typing the PRRSV isolates further.展开更多
Watermelon and melon mosaic disease is the important disease in agricultural production,mostly caused by watermelon mosaic virus.In this paper,the complete nucleotide sequence of watermelon mosaic virus Chinese isolat...Watermelon and melon mosaic disease is the important disease in agricultural production,mostly caused by watermelon mosaic virus.In this paper,the complete nucleotide sequence of watermelon mosaic virus Chinese isolate(WMV-Ch)was firstly determined.Excluding the 3’-terminal poly(A)tail,the genome was 10037 nt long,containing 5’-and 3’-terminal untranslated regions and a large open reading frame.The genome encoded a polyprotein of 3217 amino acids with a predicted Mr of 365.8 kD.Based on comparison with the proposed location of cleavage sites of other potyvirus polyproteins,10 mature proteins were predicted.There were 92.5% nucleotide and 96.4% amino acid identities over entire genome between WMV-Ch and France isolate(WMV-Fr),respectively.The nucleotide identity of the coat protein genes among WMV-Ch and WMV from different countries was all less than 95%,and phylogenetic tree revealed that WMV-Ch was most close to Japanese isolate.展开更多
文摘A full-length genome of a Chinese porcine reproductive and respiratory syndrome virus (PRRSV) isolate and designated as HB-1(sh)/2002,was sequenced and analyzed. The size of complete genome for the isolate is 15,411 nucleotides obtained from a set of overlapping cDNA clones excluding poly A tail,and is comprised of 9 ORFs,two of which encode nonstructural proteins and the others encode the structural proteins. The full-length sequence of HB-1(sh)/2002 was compared with that of two Chinese isolates,BJ-4 and CH-1a.Comparative analysis presented that the complete nucleotide sequence of HB-1(sh)/2002 shared 89.8% and 96.0% identity with BJ-4 and CH-1a,respectively.Compared with the other isolates,it seemed to be more extensive in Nsp2 of nonstructural regions and ORF5 of structural coding regions,respectively.Especially,Nsp2 only shared 77.3% and 89.1% identity with BJ-4 and CH-1a.The ORF5 shared 87.0% and 93.5% identity with BJ-4 and CH-1a.Phylogenetic analysis based on Nsp2 deduced amino acid sequence suggested that HB-1(sh)/2002 belongs to the same subgroup as CH-1a, and Nsp2 can be used as basis for monitoring the genetic variation and typing the PRRSV isolates further.
文摘Watermelon and melon mosaic disease is the important disease in agricultural production,mostly caused by watermelon mosaic virus.In this paper,the complete nucleotide sequence of watermelon mosaic virus Chinese isolate(WMV-Ch)was firstly determined.Excluding the 3’-terminal poly(A)tail,the genome was 10037 nt long,containing 5’-and 3’-terminal untranslated regions and a large open reading frame.The genome encoded a polyprotein of 3217 amino acids with a predicted Mr of 365.8 kD.Based on comparison with the proposed location of cleavage sites of other potyvirus polyproteins,10 mature proteins were predicted.There were 92.5% nucleotide and 96.4% amino acid identities over entire genome between WMV-Ch and France isolate(WMV-Fr),respectively.The nucleotide identity of the coat protein genes among WMV-Ch and WMV from different countries was all less than 95%,and phylogenetic tree revealed that WMV-Ch was most close to Japanese isolate.
