Common wheat(Triticum aestivum)is one of the most widely cultivated and consumed crops globally.In the face of limited arable land and climate changes,it is a great challenge to maintain current and increase future wh...Common wheat(Triticum aestivum)is one of the most widely cultivated and consumed crops globally.In the face of limited arable land and climate changes,it is a great challenge to maintain current and increase future wheat production.Enhancing agronomic traits in wheat by introducing mutations across all three homoeologous copies of each gene has proven to be a difficult task due to its large genome with high repetition.However,clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associ-ated nuclease(Cas)genome editing technologies offer a powerful means of precisely manipulating the genomes of crop species,thereby opening up new possibilities for biotechnology and breeding.In this review,we first focus on the development and optimization of the current CRISPR-based genome editing tools in wheat,emphasizing recent breakthroughs in precise and multiplex genome editing.We then describe the general procedure of wheat genome editing and highlight different methods to deliver the genome editing reagents into wheat cells.Furthermore,we summarize the recent applications and ad-vancements of CRISPR/Cas technologies for wheat improvement.Lastly,we discuss the remaining chal-lenges specific to wheat genome editing and its future prospects.展开更多
公路行业数字化转型发展十分迫切,数字化交付是实现工程数字化的重要手段,但目前缺少对交付过程管控方法的研究,导致目前交付成果质量与效率不高,并存在交付成果跨阶段应用率低的问题。基于CDE(Common Data Environments),研究并提出了...公路行业数字化转型发展十分迫切,数字化交付是实现工程数字化的重要手段,但目前缺少对交付过程管控方法的研究,导致目前交付成果质量与效率不高,并存在交付成果跨阶段应用率低的问题。基于CDE(Common Data Environments),研究并提出了公路工程全生命期数字化交付技术路径,明确了数据准备、信息生产及信息应用的方法与内涵,规范了数字化交付过程。进一步研究了数字化交付过程中数据准备流程、责任矩阵与实施方案编制方法、CDE平台功能架构、全生命期一模到底技术应用方法等关键技术,为公路工程全生命期数字化、智慧化发展奠定基础。展开更多
基金supported by grants from the National Key Research and Development Program of China(No.2021YFF1000800)the Frontiers Science Center for Molecular Design Breeding(No.2022TC152)+1 种基金the Hainan Yazhou Bay Seed Laboratory(No.B21HJ0504)China Agricultural University Start-up Funding.
文摘Common wheat(Triticum aestivum)is one of the most widely cultivated and consumed crops globally.In the face of limited arable land and climate changes,it is a great challenge to maintain current and increase future wheat production.Enhancing agronomic traits in wheat by introducing mutations across all three homoeologous copies of each gene has proven to be a difficult task due to its large genome with high repetition.However,clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associ-ated nuclease(Cas)genome editing technologies offer a powerful means of precisely manipulating the genomes of crop species,thereby opening up new possibilities for biotechnology and breeding.In this review,we first focus on the development and optimization of the current CRISPR-based genome editing tools in wheat,emphasizing recent breakthroughs in precise and multiplex genome editing.We then describe the general procedure of wheat genome editing and highlight different methods to deliver the genome editing reagents into wheat cells.Furthermore,we summarize the recent applications and ad-vancements of CRISPR/Cas technologies for wheat improvement.Lastly,we discuss the remaining chal-lenges specific to wheat genome editing and its future prospects.
文摘公路行业数字化转型发展十分迫切,数字化交付是实现工程数字化的重要手段,但目前缺少对交付过程管控方法的研究,导致目前交付成果质量与效率不高,并存在交付成果跨阶段应用率低的问题。基于CDE(Common Data Environments),研究并提出了公路工程全生命期数字化交付技术路径,明确了数据准备、信息生产及信息应用的方法与内涵,规范了数字化交付过程。进一步研究了数字化交付过程中数据准备流程、责任矩阵与实施方案编制方法、CDE平台功能架构、全生命期一模到底技术应用方法等关键技术,为公路工程全生命期数字化、智慧化发展奠定基础。
