Cucumber leaves at 6 leaves phase, after treated with ρ (SA)/100 mg L -1 for 0, 1, 3, 6 d, were infected by Cladosporium cucumerinum . The results showed that the leaves presented the resistance to Cladosporium cucum...Cucumber leaves at 6 leaves phase, after treated with ρ (SA)/100 mg L -1 for 0, 1, 3, 6 d, were infected by Cladosporium cucumerinum . The results showed that the leaves presented the resistance to Cladosporium cucumerinum . The strongest resistance was found in the leaves pretreated for 3 d. But, the resistance then began to decrease obviously. Even in the same pretreatment time, the resistance reflected differently in different positions of the plant. The resistance was also found in the cucumber leaf from seed pretreated by ρ (SA)/20, 100 mg L -1 . When Cladosporium cucumerinum was cultured in the medium containing SA with different concentrations, no inhibition effect on Cladosporium cucumerinum was observed in the medium with ρ (SA)/ 20, 100 mg L -1 . Only in the medium with ρ (SA)/1000 mg L -1 inhibiting effect could be observed.展开更多
Dry heat treatment has been identifie as a method for disinfecting seed-borne pathogens in vegetable seeds. This study demonstrated that three seed-borne pathogens of cucumber(Cladosporium cucumerinum that causes scab...Dry heat treatment has been identifie as a method for disinfecting seed-borne pathogens in vegetable seeds. This study demonstrated that three seed-borne pathogens of cucumber(Cladosporium cucumerinum that causes scabs, Ascochyta citrullina that results in gummy stem blight,and Colletotrichum orbiculare that induces anthracnose) could be effectively eradicated from cucumber seeds by dry heat treatment. In vitro growth of these three pathogens was inhibited by dry heat treatment at 70 °C for 40 min. These pathogens were inactivated after exposing infected seeds to 70 °C dry heat for at least 90 min. Seed infection was significant y reduced by exposing the seeds to 70 °C dry heat for at least 40 min. Seed moisture content and germination were slightly reduced after 70 °C heat treatment for 40–120 min. Seed vigor remained at a high level after dry heat treatment at 70 °C for 90 min. In conclusion, 70 °C dry heat treatment for 90 min was determined to be the optimal method for eradication of C. cucumerinum, Didymella bryoniae, and C. orbiculare from cucumber seeds.展开更多
报道了一种新的黄瓜黑星病抗病性鉴定方法——离体子叶接种法,并对接种鉴定条件进行了研究。该方法将刚展平的子叶剪下铺到有二层滤纸的培养皿内保湿,(?)滴管将10~4～10~5个·mL^(-1)孢子浓度的悬浮液,点滴接种到离体子叶的上表皮,...报道了一种新的黄瓜黑星病抗病性鉴定方法——离体子叶接种法,并对接种鉴定条件进行了研究。该方法将刚展平的子叶剪下铺到有二层滤纸的培养皿内保湿,(?)滴管将10~4～10~5个·mL^(-1)孢子浓度的悬浮液,点滴接种到离体子叶的上表皮,每滴约0.02 mL.在15～20℃,黑暗条件下鉴定,接种后5 d 调查病情。这一方法简单、可靠、不影响幼苗继续生长。展开更多
文摘Cucumber leaves at 6 leaves phase, after treated with ρ (SA)/100 mg L -1 for 0, 1, 3, 6 d, were infected by Cladosporium cucumerinum . The results showed that the leaves presented the resistance to Cladosporium cucumerinum . The strongest resistance was found in the leaves pretreated for 3 d. But, the resistance then began to decrease obviously. Even in the same pretreatment time, the resistance reflected differently in different positions of the plant. The resistance was also found in the cucumber leaf from seed pretreated by ρ (SA)/20, 100 mg L -1 . When Cladosporium cucumerinum was cultured in the medium containing SA with different concentrations, no inhibition effect on Cladosporium cucumerinum was observed in the medium with ρ (SA)/ 20, 100 mg L -1 . Only in the medium with ρ (SA)/1000 mg L -1 inhibiting effect could be observed.
基金supported by the earmarked fund for the Science and Technology Innovation Program of the Chinese Academy of Agricultural Science(CAAS-ASTIP-IVFCAAS)the National Key Technology Support Program(2012BAD19B06)funded by the Key Laboratory of Horticultural Crops Genetic Improvement,Ministry of Agriculture in China
文摘Dry heat treatment has been identifie as a method for disinfecting seed-borne pathogens in vegetable seeds. This study demonstrated that three seed-borne pathogens of cucumber(Cladosporium cucumerinum that causes scabs, Ascochyta citrullina that results in gummy stem blight,and Colletotrichum orbiculare that induces anthracnose) could be effectively eradicated from cucumber seeds by dry heat treatment. In vitro growth of these three pathogens was inhibited by dry heat treatment at 70 °C for 40 min. These pathogens were inactivated after exposing infected seeds to 70 °C dry heat for at least 90 min. Seed infection was significant y reduced by exposing the seeds to 70 °C dry heat for at least 40 min. Seed moisture content and germination were slightly reduced after 70 °C heat treatment for 40–120 min. Seed vigor remained at a high level after dry heat treatment at 70 °C for 90 min. In conclusion, 70 °C dry heat treatment for 90 min was determined to be the optimal method for eradication of C. cucumerinum, Didymella bryoniae, and C. orbiculare from cucumber seeds.
文摘报道了一种新的黄瓜黑星病抗病性鉴定方法——离体子叶接种法,并对接种鉴定条件进行了研究。该方法将刚展平的子叶剪下铺到有二层滤纸的培养皿内保湿,(?)滴管将10~4～10~5个·mL^(-1)孢子浓度的悬浮液,点滴接种到离体子叶的上表皮,每滴约0.02 mL.在15～20℃,黑暗条件下鉴定,接种后5 d 调查病情。这一方法简单、可靠、不影响幼苗继续生长。
