In-vitro callus induction and regeneration method was developed using different plant growth regulators (PGRs), and basal media (Murashige and Skoog (MS), CHU (N6) and Gamborg (B5) media) of Citrus sinensis (L.) Osbec...In-vitro callus induction and regeneration method was developed using different plant growth regulators (PGRs), and basal media (Murashige and Skoog (MS), CHU (N6) and Gamborg (B5) media) of Citrus sinensis (L.) Osbeck. Observations of the effect of PGRs were carried out using different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D),1-naphthalene acetic acid (NAA) and combinations of 2,4-D and NAA using different basal media. This study found Citrus sinensis (L.) Osbeck exhibited a high frequency of callus induction on MS medium supplemented with 3 mg/L 2,4-D and callus induction frequency was 86.7% ± 3.4% whereas N6 and B5 showed lower callus induction frequency of 83.3% ± 8.8% and 82.2% ± 1.9% respectively compared to that of MS media with supplementation of the same hormone. Among the induced calli, the morphological analysis showed only 40% - 50% was embryogenic calli. Regeneration of plantlets from calli was done using different concentrations and combinations of auxin and cytokinin. The study showed that 3 mg/L 6-benzylaminopurine (BAP) supplemented medium has the maximum potential to promote regeneration of Citrus sinensis (L.) Osbeck from embryogenic calli with the frequency of 89.3% ± 8.8% but no regeneration occurred from the non-embryogenic calli. The regenerated plantlets were rooted on MS medium with supplementation of 5 mg/l NAA. These observations in Citrus sinensis (L.) Osbeck regeneration will be helpful for genetic improvement with desired traits.展开更多
为降低非浓缩还原(not from concentrate,NFC)橙汁储运销成本并提高其贮藏品质,以长叶香橙为原料,脱气杀菌后分别灌装至玻璃(glass,GL)、聚丙烯(polypropylene,PP)和聚乙烯(polyethylene,PE)(透氧率:GL<PP<PE)材质的包装中,25℃...为降低非浓缩还原(not from concentrate,NFC)橙汁储运销成本并提高其贮藏品质,以长叶香橙为原料,脱气杀菌后分别灌装至玻璃(glass,GL)、聚丙烯(polypropylene,PP)和聚乙烯(polyethylene,PE)(透氧率:GL<PP<PE)材质的包装中,25℃下避光贮藏并定期测定溶解氧(dissolved oxygen,DO)含量和相关品质指标。结果显示,DO值变化速度与包装材料的透氧率差异一致,GL、PP和PE中的还原糖含量均先降后升,可接受感官品质的贮藏时间分别为60、15和7 d,VC含量和色泽指标(ΔE,A_(420))均与包装的透氧率呈极显著相关(P<0.01),类黄酮和酚酸化合物含量与包装透氧性不具有显著相关性。GL瓶中橙汁DO值在120 d内稳定在0.5 mg/L左右,其橙汁贮藏效果最佳。结果表明,在贮藏期间将DO值控制在0.5 mg/L及以下可更好地保持NFC橙汁的品质,实现NFC橙汁的常温储运。展开更多
文摘In-vitro callus induction and regeneration method was developed using different plant growth regulators (PGRs), and basal media (Murashige and Skoog (MS), CHU (N6) and Gamborg (B5) media) of Citrus sinensis (L.) Osbeck. Observations of the effect of PGRs were carried out using different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D),1-naphthalene acetic acid (NAA) and combinations of 2,4-D and NAA using different basal media. This study found Citrus sinensis (L.) Osbeck exhibited a high frequency of callus induction on MS medium supplemented with 3 mg/L 2,4-D and callus induction frequency was 86.7% ± 3.4% whereas N6 and B5 showed lower callus induction frequency of 83.3% ± 8.8% and 82.2% ± 1.9% respectively compared to that of MS media with supplementation of the same hormone. Among the induced calli, the morphological analysis showed only 40% - 50% was embryogenic calli. Regeneration of plantlets from calli was done using different concentrations and combinations of auxin and cytokinin. The study showed that 3 mg/L 6-benzylaminopurine (BAP) supplemented medium has the maximum potential to promote regeneration of Citrus sinensis (L.) Osbeck from embryogenic calli with the frequency of 89.3% ± 8.8% but no regeneration occurred from the non-embryogenic calli. The regenerated plantlets were rooted on MS medium with supplementation of 5 mg/l NAA. These observations in Citrus sinensis (L.) Osbeck regeneration will be helpful for genetic improvement with desired traits.