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马蜂柑响应黄龙病菌不同侵染时期的生物学和转录组学分析 被引量:5
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作者 滕彩玲 钟晰 +3 位作者 吴昊娣 胡燕 周常勇 王雪峰 《中国农业科学》 CAS CSCD 北大核心 2020年第7期1368-1380,共13页
【目的】柑橘黄龙病(Huanglongbing,HLB)是制约柑橘产业发展的重大病害,我国发生的黄龙病由韧皮部杆菌亚洲种(‘Candidatus Liberibacter asiaticus’,CLas)引起。本研究通过分析潜在耐黄龙病柑橘品种马蜂柑(Citrus hystrix)在感染黄龙... 【目的】柑橘黄龙病(Huanglongbing,HLB)是制约柑橘产业发展的重大病害,我国发生的黄龙病由韧皮部杆菌亚洲种(‘Candidatus Liberibacter asiaticus’,CLas)引起。本研究通过分析潜在耐黄龙病柑橘品种马蜂柑(Citrus hystrix)在感染黄龙病后不同时期的生物学症状、显微结构和转录组学差异,揭示马蜂柑不同时期对黄龙病菌具有耐性的分子机理,为进一步深入挖掘抗性基因及开展抗病育种打下基础。【方法】以嫁接在两年生卡里佐枳橙砧木上的马蜂柑作为供试材料,使用只感染CLas、其他常见柑橘病毒类病原均呈阴性的毒源为接毒材料对马蜂柑进行接种,并在接种毒源后每隔15 d进行定期的荧光定量PCR检测。将马蜂柑接种毒源4个月(最早检测到CLas阳性)作为感病前期处理,接种毒源14个月作为感病后期处理,以健康植株为对照,进行生物学症状和显微结构观察,分析其感病后不同时期的结构变化。结合比较转录组学分析与荧光定量PCR验证,解析其耐病相关机制。【结果】症状观察发现,马蜂柑在感病前期和感病后期,植株叶片几乎不显症;显微结构观察表明,马蜂柑在感病前期中脉组织结构清晰,细胞形态正常,无淀粉粒积累的现象,仅在后期出现韧皮部极少数的筛管被堵塞;对转录组测序结果进行筛选鉴定,马蜂柑感病前期共筛选鉴定到181个差异表达基因,感病后期共筛选鉴定到1384个差异表达基因;比较转录组分析表明,马蜂柑感病前期和后期的差异表达基因主要涉及细胞壁代谢、防御反应、淀粉与蔗糖代谢、胼胝质合成以及信号转导等方面,其中在感病前期,淀粉与蔗糖代谢、细胞壁代谢相关的调控基因下调表达,在感病后期,水杨酸代谢及其信号转导途径、病程相关蛋白和谷胱甘肽转移酶相关的调控基因上调表达。【结论】马蜂柑响应CLas早期侵染主要表现为物理结构稳定、淀� 展开更多
关键词 柑橘黄龙病 韧皮部杆菌亚洲种 马蜂柑 耐病性 转录组
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Oviposition preference of swallowtail butterfly, Papilio polytes (Lepidoptera Papilionidae) on four Rutaceae (Sapindales) host plant species 被引量:1
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作者 Suwarno M.R. Che Salmah +1 位作者 Arshad Ali A. Abu Hassan 《Insect Science》 SCIE CAS CSCD 2010年第4期369-378,共10页
Oviposition preference and egg deposition site selection by the butterfly, Papilio polytes L. (Lepidoptera: Papilionidae) on four rutaceous host plants, Citrus aurantofolia (Christm.) Swing., Citrus hystrix DC., ... Oviposition preference and egg deposition site selection by the butterfly, Papilio polytes L. (Lepidoptera: Papilionidae) on four rutaceous host plants, Citrus aurantofolia (Christm.) Swing., Citrus hystrix DC., Citrus reticulata Blanco, and Murraya koenigii (L.) Sprengel, were assessed in field cages measuring 2 × 2 ×2 m. Simultaneous two-choice and four-choice oviposition tests of whole host plants were conducted. The mean total number of eggs laid per plant on C. reticulata in the two-choice test was significantly higher (P 〈 0.01) than those on C. aurantifolia, C. hystrix, andM. koenigii. Among the three other host plants, C. aurantifolia was preferred over C. hystrix, and M. koenigii (P 〈 0.01) and C. hystrix was preferred over M. koenigii (P 〈 0.01). In the four-choice test, C. reticulata was highly preferred and significantly different from C. hystrix and M. koenigii. However, no oviposition preference was detected with C. aurantifolia. Among the various plant parts in the two- and four-choice tests, eggs on leaves of each plant were the highest, followed by numbers on stems, and negligible numbers on pots. These numbers on leaves of C. reticulata and C. aurantifolia were not significantly different (P 〉 0.05), but differed significantly (P 〈 0.01) from those on leaves of C. hystrix and M. koenigii. The quantitative trend of egg-laying on stems was very similar to that observed for the leaves. Papilio polytes showed strong preference to lay eggs on the underside of leaves of all host plants than on the upper side or on the petiole. More eggs were laid on the upper side of each host plant than on its petiole. The four host plants in descending order of preference were C. reticulata 〉 C. aurantifolia 〉 C. hystrix 〉 M. koenigii. Although M. koenigii was the least preferred, it has the potential to serve as an alternative host plant for P. polytes which can be manipulated when necessary, to alleviate the infestation of this pest to the citrus industry. 展开更多
关键词 citrus aurantifolia citrus hystrix citrus reticulata Murraya koenigii ovipo-sition preference Papilio polytes
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Cardioprotective and hepatoprotective effects of Citrus hystrix peels extract on rats model 被引量:1
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作者 Herwandhani Putri Standie Nagadi +2 位作者 Yonika Arum Larasati Nindi Wulandari Adam Hermawan 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2013年第5期371-375,共5页
Objective:To observe the combination effect of doxorubicin and Citrus hystrix(kaffir lime's)peel ethanolic extract(ChEE)on blood serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)activity and c... Objective:To observe the combination effect of doxorubicin and Citrus hystrix(kaffir lime's)peel ethanolic extract(ChEE)on blood serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)activity and cardio-hepato-histopathology of female Sprague Dawley rats.Methods:Doxorubicin and ChEE(5 rats per group)were administered in five groups of 3 rats each for 11 d.Group I:doxorubicin(dox)4.67 mg/kg body weight;Group II:dox+ChEE 500 mg/kg body weight;Group HI:dox+ChEE 1000 mg/kg body weight;Group IV:ChEE 1000 mg/kg body weight;Group V:untreated(control).Results:ChEE repaired cardiohistopathology profile of doxorubicin induced cardiotoxicity and hepatotoxicity rats,but did not repair neither hepatohistopathology profile nor reduce serum activity of ALT and AST.Conclusion:ChEE has potency to be developed as cardioprotector agent in chemotherapy. 展开更多
关键词 citrus hystrix D.C. Cardiohepatoprotector HISTOPATHOLOGY Chemoterapy
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