目的:观察新型顺铂植入剂(cisplatin forinjection)与常规顺铂注射液在人胃癌裸鼠移植瘤模型中促肿瘤细胞凋亡以及对脾酪氨酸激酶(spleen tyrosine kinase,SYK)、人表皮生长因子受体2(human epidermal growth factorreceptor 2,HER2)、...目的:观察新型顺铂植入剂(cisplatin forinjection)与常规顺铂注射液在人胃癌裸鼠移植瘤模型中促肿瘤细胞凋亡以及对脾酪氨酸激酶(spleen tyrosine kinase,SYK)、人表皮生长因子受体2(human epidermal growth factorreceptor 2,HER2)、黏附分子CD44的变异体6(CD44v6)、人第10号染色体缺失的磷酸酶及张力蛋白同源的基因(phosphate and tensionhomology deleted on chromsome ten,PTEN)、含半胱氨酸的天冬氨酸蛋白水解酶3(cysteinylaspartate specific protease 3,Caspase3)、存活素或凋亡抑制基因(Survivin)在胃癌组织中表达水平的影响差异,探讨新型顺铂植入剂在胃癌发生发展过程中的治疗优势.方法:用人胃癌SGC7901细胞株建立Balb/c裸鼠皮下移植瘤模型,随机分4组:PBS尾静脉注射组(A组)、顺铂尾静脉注射组(B组)、顺铂瘤内注射组(C组),顺铂植入剂植入组(D组),每组5只.经药物干预后,记录瘤质量,计算抑瘤率,并对瘤体进行HE染色,通过免疫组织化学法检测SYK、HER2、CD44v6、PTEN、Caspase3、Survivin表达水平,流式细胞术检测各组细胞凋亡情况.结果:与对照组比较后发现新型顺铂植入剂能显著抑制移植瘤生长,促进其凋亡并能较显著提高SYK、PTEN、Caspase3的表达:SYK的表达[D组与C组比较(73.42±4.92 vs 30.42±3.92),差异有统计学意义(P<0.05);而B组与A组比较(14.14±2.84 vs 5.06±2.96),差异有统计学意义(P<0.05)],PTEN的表达[D组与C组比较(62.46±4.36 vs 29.82±2.22),差异有统计学意义(P<0.05);而B组与A组比较(16.06±4.26 vs 6.44±2.84),差异有统计学意义(P<0.05)]、Caspase3的表达[D组与C组比较(77.58±6.28 vs 31.26±4.76),差异有统计学意义(P<0.05);而B组与A组比较(15.24±4.64vs 10.82±2.72),差异有统计学意义(P<0.05)],降低HER2、CD44v6、Survivin表达.HER2的表达[D组与C组比较(16.32±4.82 vs 34.82±7.32),差异有统计学意义(P<0.05);而B组与A组比较(45.8±6.60 vs 77.34±9.04),差异有统计学意义(P<0.05)],CD44v6的�展开更多
Objective: To study the influence of cisplatin implants on transplantation tumor growth and the expression of tissue kallikrein-7(KLK7) and E-cadherin(E-cad) in tumor-bearing mice with gastric cancer. Methods: BALB/c ...Objective: To study the influence of cisplatin implants on transplantation tumor growth and the expression of tissue kallikrein-7(KLK7) and E-cadherin(E-cad) in tumor-bearing mice with gastric cancer. Methods: BALB/c nude mice were collected as experimental animal and were randomly divided into model control group(Group A), tail intravenous injection of cisplatin group(Group B), intratumor injection of cisplatin group(Group C) and cisplatin implants treatment group(Group D). After the drugs intervening, the weight and volume of transplantation tumors were measured on Day 20, Day 30 and Day 40 and serum and KLK7 and E-cad contents in transplanted tumor tissue were examined. Results: On Day 20, Day 30 and Day 40 after treatment, the weight and volume of transplantation tumors of tumorbearing mice in four groups were different(Group A > Group B > Group C > Group D). The contents of KLK-7 and E-cad in tumor tissue and serum of tumor-bearing mice in four groups were different(Group A > Group B > Group C > Group D in KLK-7) and(Group A < Group B < Group C < Group D in E-cad). The weight and volume, and KLK7 and E-cad contents of transplantation tumors in four groups were significant difference(P<0.05). Conclusion: Cisplatin implants can inhibit the growth of transplanted tumor tissue and down-regulated KLK7 expression and up-regulated E-cad expression of tumor-bearing mice with gastric展开更多
文摘目的:观察新型顺铂植入剂(cisplatin forinjection)与常规顺铂注射液在人胃癌裸鼠移植瘤模型中促肿瘤细胞凋亡以及对脾酪氨酸激酶(spleen tyrosine kinase,SYK)、人表皮生长因子受体2(human epidermal growth factorreceptor 2,HER2)、黏附分子CD44的变异体6(CD44v6)、人第10号染色体缺失的磷酸酶及张力蛋白同源的基因(phosphate and tensionhomology deleted on chromsome ten,PTEN)、含半胱氨酸的天冬氨酸蛋白水解酶3(cysteinylaspartate specific protease 3,Caspase3)、存活素或凋亡抑制基因(Survivin)在胃癌组织中表达水平的影响差异,探讨新型顺铂植入剂在胃癌发生发展过程中的治疗优势.方法:用人胃癌SGC7901细胞株建立Balb/c裸鼠皮下移植瘤模型,随机分4组:PBS尾静脉注射组(A组)、顺铂尾静脉注射组(B组)、顺铂瘤内注射组(C组),顺铂植入剂植入组(D组),每组5只.经药物干预后,记录瘤质量,计算抑瘤率,并对瘤体进行HE染色,通过免疫组织化学法检测SYK、HER2、CD44v6、PTEN、Caspase3、Survivin表达水平,流式细胞术检测各组细胞凋亡情况.结果:与对照组比较后发现新型顺铂植入剂能显著抑制移植瘤生长,促进其凋亡并能较显著提高SYK、PTEN、Caspase3的表达:SYK的表达[D组与C组比较(73.42±4.92 vs 30.42±3.92),差异有统计学意义(P<0.05);而B组与A组比较(14.14±2.84 vs 5.06±2.96),差异有统计学意义(P<0.05)],PTEN的表达[D组与C组比较(62.46±4.36 vs 29.82±2.22),差异有统计学意义(P<0.05);而B组与A组比较(16.06±4.26 vs 6.44±2.84),差异有统计学意义(P<0.05)]、Caspase3的表达[D组与C组比较(77.58±6.28 vs 31.26±4.76),差异有统计学意义(P<0.05);而B组与A组比较(15.24±4.64vs 10.82±2.72),差异有统计学意义(P<0.05)],降低HER2、CD44v6、Survivin表达.HER2的表达[D组与C组比较(16.32±4.82 vs 34.82±7.32),差异有统计学意义(P<0.05);而B组与A组比较(45.8±6.60 vs 77.34±9.04),差异有统计学意义(P<0.05)],CD44v6的�
基金supported by Health Department of Hainan Province(Qiong 2013-self-financing 08)
文摘Objective: To study the influence of cisplatin implants on transplantation tumor growth and the expression of tissue kallikrein-7(KLK7) and E-cadherin(E-cad) in tumor-bearing mice with gastric cancer. Methods: BALB/c nude mice were collected as experimental animal and were randomly divided into model control group(Group A), tail intravenous injection of cisplatin group(Group B), intratumor injection of cisplatin group(Group C) and cisplatin implants treatment group(Group D). After the drugs intervening, the weight and volume of transplantation tumors were measured on Day 20, Day 30 and Day 40 and serum and KLK7 and E-cad contents in transplanted tumor tissue were examined. Results: On Day 20, Day 30 and Day 40 after treatment, the weight and volume of transplantation tumors of tumorbearing mice in four groups were different(Group A > Group B > Group C > Group D). The contents of KLK-7 and E-cad in tumor tissue and serum of tumor-bearing mice in four groups were different(Group A > Group B > Group C > Group D in KLK-7) and(Group A < Group B < Group C < Group D in E-cad). The weight and volume, and KLK7 and E-cad contents of transplantation tumors in four groups were significant difference(P<0.05). Conclusion: Cisplatin implants can inhibit the growth of transplanted tumor tissue and down-regulated KLK7 expression and up-regulated E-cad expression of tumor-bearing mice with gastric
