Gastric cancer(GC)is one of the most common malignancies,with an everincreasing incidence and high mortality rate.Chromobox4(CBX4),also named hPC2,is a small ubiquitin-related modifier(SUMO)E3 ligase.Previous studies ...Gastric cancer(GC)is one of the most common malignancies,with an everincreasing incidence and high mortality rate.Chromobox4(CBX4),also named hPC2,is a small ubiquitin-related modifier(SUMO)E3 ligase.Previous studies have found that high CBX4 expression is associated with tumor size,pathologic differentiation and decreased patient survival in hepatocellular carcinoma(HCC).However,the expression and prognostic value of CBX4 in GC have not been clarified.In our study,ONCOMINE,UALCAN,KaplanMeier Plotter,cBioPortal,DAVID 6.8 and TIMER were utilized.RT-PCR,immunohistochemistry(IHC),Western blot,CCK-8 assay,cell apoptosis assay,cell cycle assay were used to further verify in GC tissue samples or cell line.The transcriptional and protein level of CBX4 in GC tissues was found significantly elevated and a significant association between the expression of CBX4 and clinicopathological parameters was found in GC patients.Low expression of CBX4 in GC patients were correlated with a significantly improved prognosis.The functions of CBX4 are primarily related to the stem cell pluripotency signaling pathway,Hippo signaling pathway,HTLV-I infection,Notch signaling pathway,and N-glycan biosynthesis.Our results may provide novel insights for the selection of therapeutic targets and prognostic biomarkers for GC.展开更多
基金This project was supported by the National Natural Science Foundation of China[grant number 81972655]the Program for Young Eastern Scholars at the Shanghai Institutions of Higher Learning[grant number QD2016004]+1 种基金the Shanghai Science and Technology Commission Research Project[grant number 14441903103]Shanghai Municipal Key Clinic Specialty.
文摘Gastric cancer(GC)is one of the most common malignancies,with an everincreasing incidence and high mortality rate.Chromobox4(CBX4),also named hPC2,is a small ubiquitin-related modifier(SUMO)E3 ligase.Previous studies have found that high CBX4 expression is associated with tumor size,pathologic differentiation and decreased patient survival in hepatocellular carcinoma(HCC).However,the expression and prognostic value of CBX4 in GC have not been clarified.In our study,ONCOMINE,UALCAN,KaplanMeier Plotter,cBioPortal,DAVID 6.8 and TIMER were utilized.RT-PCR,immunohistochemistry(IHC),Western blot,CCK-8 assay,cell apoptosis assay,cell cycle assay were used to further verify in GC tissue samples or cell line.The transcriptional and protein level of CBX4 in GC tissues was found significantly elevated and a significant association between the expression of CBX4 and clinicopathological parameters was found in GC patients.Low expression of CBX4 in GC patients were correlated with a significantly improved prognosis.The functions of CBX4 are primarily related to the stem cell pluripotency signaling pathway,Hippo signaling pathway,HTLV-I infection,Notch signaling pathway,and N-glycan biosynthesis.Our results may provide novel insights for the selection of therapeutic targets and prognostic biomarkers for GC.
