In the pathophysiology of neurodegenerative disorders,the role of misfolded protein deposition leading to neurodegeneration has been primarily discussed.In the last decade,however,it has been proposed a parallel invol...In the pathophysiology of neurodegenerative disorders,the role of misfolded protein deposition leading to neurodegeneration has been primarily discussed.In the last decade,however,it has been proposed a parallel involvement of innate immune activation,chronic inflammation and adaptive immunity in the neurodegeneration mechanisms triggered by proteinopathies.New insights in the neurodegenerative field strongly suggest a role for the immune system in the pathophysiology of neurodegenerative disorders.Therefore,the hypothesis underlining the modulation of the innate and the adaptive immune system in the events linked to brain deposition of misfolded proteins could open new perspectives in the setting of specific immunotherapeutic strategies for the treatment of neurodegenerative diseases.Therefore,we have reviewed the pathogenic hypothesis in neurodegenerative pathologies,underling the links between the deposition of misfolded protein mechanisms and the immune activation.展开更多
We examined a new method for visualization of the primo vascular system in the rat brain involving lateral ventricle injection of trypan blue. Results showed that the primo vascular system in the lateral ventricles an...We examined a new method for visualization of the primo vascular system in the rat brain involving lateral ventricle injection of trypan blue. Results showed that the primo vascular system in the lateral ventricles and arachnoid mater of the brain were preferentially stained relative to blood vessels and fascia. The primo-vessels along blood vessels in the brain were clearly exhibited. In addition, the primo vascular system was evident between the fourth ventricle and the quadrigeminal cistern. Our experimental findings indicate that this new technique of lateral ventricle injection of trypan blue can visualize the primo vascular system in lateral ventricles and arachnoid mater of rats in situ.展开更多
老龄化可引起铁元素在脑内的蓄积。随着年龄的增加,脉络丛上皮可能是脑铁毒性、炎症反应和氧化应激损伤的重要结构。本文旨在研究铁代谢调节中的关键激素——铁调素在脉络丛上皮表达的年龄变化,及其与白细胞介素-6（interleukin-6,IL-6...老龄化可引起铁元素在脑内的蓄积。随着年龄的增加,脉络丛上皮可能是脑铁毒性、炎症反应和氧化应激损伤的重要结构。本文旨在研究铁代谢调节中的关键激素——铁调素在脉络丛上皮表达的年龄变化,及其与白细胞介素-6（interleukin-6,IL-6）和信号转导子及转录激活子3（signal transducer and activator of transcription 3,Stat3）的关系。本文以不同年龄段（3、6、9、12、15、18、21、24、27、30、33和36月龄）大鼠为研究对象,用实时定量RT-PCR和Western blot法检测铁调素及IL-6/Stat3信号途径相关蛋白在脉络丛上皮的表达变化。结果显示,3~18月龄年轻大鼠脉络丛上皮铁调素m RNA没有明显变化,21~36月龄大鼠的铁调素m RNA水平显著增加。脉络丛上皮IL-6和p-Stat3 m RNA和蛋白表达的年龄变化趋势与铁调素m RNA一致。以上结果提示,随着年龄的增加,炎症因子IL-6可能作用于相应的受体,通过Stat3信号转导途径,参与调节铁调素在脉络丛上皮的表达变化。展开更多
文摘In the pathophysiology of neurodegenerative disorders,the role of misfolded protein deposition leading to neurodegeneration has been primarily discussed.In the last decade,however,it has been proposed a parallel involvement of innate immune activation,chronic inflammation and adaptive immunity in the neurodegeneration mechanisms triggered by proteinopathies.New insights in the neurodegenerative field strongly suggest a role for the immune system in the pathophysiology of neurodegenerative disorders.Therefore,the hypothesis underlining the modulation of the innate and the adaptive immune system in the events linked to brain deposition of misfolded proteins could open new perspectives in the setting of specific immunotherapeutic strategies for the treatment of neurodegenerative diseases.Therefore,we have reviewed the pathogenic hypothesis in neurodegenerative pathologies,underling the links between the deposition of misfolded protein mechanisms and the immune activation.
基金the National Natural Science Foundation of China for Youths (General Program),No.30801464the National Basic Research Program of China (973 Program),No.2007CB512705the GIST Systems Biology Program of Korea in 2010
文摘We examined a new method for visualization of the primo vascular system in the rat brain involving lateral ventricle injection of trypan blue. Results showed that the primo vascular system in the lateral ventricles and arachnoid mater of the brain were preferentially stained relative to blood vessels and fascia. The primo-vessels along blood vessels in the brain were clearly exhibited. In addition, the primo vascular system was evident between the fourth ventricle and the quadrigeminal cistern. Our experimental findings indicate that this new technique of lateral ventricle injection of trypan blue can visualize the primo vascular system in lateral ventricles and arachnoid mater of rats in situ.
基金supported by the Natural Science Foundation of Zhejiang Province,China(No.Y2110388)the Science and Technology Project of Zhejiang Province,China(No.Y2011C37091)
文摘老龄化可引起铁元素在脑内的蓄积。随着年龄的增加,脉络丛上皮可能是脑铁毒性、炎症反应和氧化应激损伤的重要结构。本文旨在研究铁代谢调节中的关键激素——铁调素在脉络丛上皮表达的年龄变化,及其与白细胞介素-6（interleukin-6,IL-6）和信号转导子及转录激活子3（signal transducer and activator of transcription 3,Stat3）的关系。本文以不同年龄段（3、6、9、12、15、18、21、24、27、30、33和36月龄）大鼠为研究对象,用实时定量RT-PCR和Western blot法检测铁调素及IL-6/Stat3信号途径相关蛋白在脉络丛上皮的表达变化。结果显示,3~18月龄年轻大鼠脉络丛上皮铁调素m RNA没有明显变化,21~36月龄大鼠的铁调素m RNA水平显著增加。脉络丛上皮IL-6和p-Stat3 m RNA和蛋白表达的年龄变化趋势与铁调素m RNA一致。以上结果提示,随着年龄的增加,炎症因子IL-6可能作用于相应的受体,通过Stat3信号转导途径,参与调节铁调素在脉络丛上皮的表达变化。
