[目的]通过白藜芦醇刺激体外培养的小鼠膝关节软骨细胞,探讨白藜芦醇通过PI3K/Akt信号通路对软骨细胞细胞外基质合成的影响。[方法]体外分离培养小鼠膝关节软骨细胞,采用HE染色、甲苯胺蓝染色和Ⅱ型胶原免疫荧光染色观察鉴定软骨细胞。...[目的]通过白藜芦醇刺激体外培养的小鼠膝关节软骨细胞,探讨白藜芦醇通过PI3K/Akt信号通路对软骨细胞细胞外基质合成的影响。[方法]体外分离培养小鼠膝关节软骨细胞,采用HE染色、甲苯胺蓝染色和Ⅱ型胶原免疫荧光染色观察鉴定软骨细胞。根据作用药物的不同分为3组:空白对照组、白藜芦醇组(100μmol/L白藜芦醇)和LY294002组(100μmol/L白藜芦醇+25μmol/L LY294002)。采用RT-PCR检测软骨细胞中Akt1,Aggrecan、Collagen II的m RNA表达水平。[结果]荧光显微镜下可见软骨细胞胞核呈蓝色荧光,胞质呈红色荧光。显示Ⅱ型胶原染色阳性细胞数占90%以上,证实所培养细胞为软骨细胞。RT-PCR检测结果显示白藜芦醇组Akt1、Aggrecan、Collagen II m RNA表达量较空白组明显升高(P<0.05)。LY294002组Akt1、Aggrecan、Collagen II m RNA表达量较白藜芦醇组下降,但仍高于空白组(P<0.05)。[结论]白藜芦醇可以通过激活PI3K/Akt信号通路,增加软骨细胞细胞外基质合成,起到保护关节软骨的作用。展开更多
Kaschin-Beck disease （KBD）, endemic in China, occurs along a low selenium belt where the Se contents of water, soil, crops and patients’ blood and hair are all in a low state. Supplementation of Na<sub>2</...Kaschin-Beck disease （KBD）, endemic in China, occurs along a low selenium belt where the Se contents of water, soil, crops and patients’ blood and hair are all in a low state. Supplementation of Na<sub>2</sub>SeO<sub>3</sub> has been effective in preventing such a disease. The main pathological changes of KBD are the necrosis of cartilage, early展开更多
The effects of various cartilage extracellular matrix on the construction of rabbit growth plate cartilage tissue in vitro were studied. The results show that collagen, proteoglycan and hyaluronic acid can promote the...The effects of various cartilage extracellular matrix on the construction of rabbit growth plate cartilage tissue in vitro were studied. The results show that collagen, proteoglycan and hyaluronic acid can promote the growth of cultured chondrocytes but the effects of various cartilage extracellular matrix(ECM)on chondrocyte differentiation are different. Collagen can promote the hypertrophy of chondrocytes while proteoglycan and hyaluronic acid inhibit the transition of mature chondrocytes into hypertrophied chondrocytes.展开更多
The effects of simulated microgravity on cell growth and extracellular matrix of chondrocytes have been examined by a light microscope and a scanning electron microscope using cultured chicken embryonic chondrocytes a...The effects of simulated microgravity on cell growth and extracellular matrix of chondrocytes have been examined by a light microscope and a scanning electron microscope using cultured chicken embryonic chondrocytes as the model. No notable difference of the cell density between the rotation group and the control group has been found. But during the same period,the growth spots of the rotation group were sparser than those of the control group. These results indicated that the lower level of cell development and differentiation happened in the rotation group. Observation by the scanning electron microscope showed that the extracellualr matrix decreased after rotating, and the fibres in the extracellular matrix were slighter and blurrier than those of the control group. It is concluded that the simulated microgravity can affect the secreting and assembly of the extracellular matrix. The possible mechanism for them is discussed.展开更多
文摘[目的]通过白藜芦醇刺激体外培养的小鼠膝关节软骨细胞,探讨白藜芦醇通过PI3K/Akt信号通路对软骨细胞细胞外基质合成的影响。[方法]体外分离培养小鼠膝关节软骨细胞,采用HE染色、甲苯胺蓝染色和Ⅱ型胶原免疫荧光染色观察鉴定软骨细胞。根据作用药物的不同分为3组:空白对照组、白藜芦醇组(100μmol/L白藜芦醇)和LY294002组(100μmol/L白藜芦醇+25μmol/L LY294002)。采用RT-PCR检测软骨细胞中Akt1,Aggrecan、Collagen II的m RNA表达水平。[结果]荧光显微镜下可见软骨细胞胞核呈蓝色荧光,胞质呈红色荧光。显示Ⅱ型胶原染色阳性细胞数占90%以上,证实所培养细胞为软骨细胞。RT-PCR检测结果显示白藜芦醇组Akt1、Aggrecan、Collagen II m RNA表达量较空白组明显升高(P<0.05)。LY294002组Akt1、Aggrecan、Collagen II m RNA表达量较白藜芦醇组下降,但仍高于空白组(P<0.05)。[结论]白藜芦醇可以通过激活PI3K/Akt信号通路,增加软骨细胞细胞外基质合成,起到保护关节软骨的作用。
文摘Kaschin-Beck disease （KBD）, endemic in China, occurs along a low selenium belt where the Se contents of water, soil, crops and patients’ blood and hair are all in a low state. Supplementation of Na<sub>2</sub>SeO<sub>3</sub> has been effective in preventing such a disease. The main pathological changes of KBD are the necrosis of cartilage, early
基金This work was supported by a grant from Foundation of Education Department of China (NO.02190) and the Science and Technology Foundation of Guang-zhou City (NO.2002 Z1 -E0032)
文摘The effects of various cartilage extracellular matrix on the construction of rabbit growth plate cartilage tissue in vitro were studied. The results show that collagen, proteoglycan and hyaluronic acid can promote the growth of cultured chondrocytes but the effects of various cartilage extracellular matrix(ECM)on chondrocyte differentiation are different. Collagen can promote the hypertrophy of chondrocytes while proteoglycan and hyaluronic acid inhibit the transition of mature chondrocytes into hypertrophied chondrocytes.
文摘The effects of simulated microgravity on cell growth and extracellular matrix of chondrocytes have been examined by a light microscope and a scanning electron microscope using cultured chicken embryonic chondrocytes as the model. No notable difference of the cell density between the rotation group and the control group has been found. But during the same period,the growth spots of the rotation group were sparser than those of the control group. These results indicated that the lower level of cell development and differentiation happened in the rotation group. Observation by the scanning electron microscope showed that the extracellualr matrix decreased after rotating, and the fibres in the extracellular matrix were slighter and blurrier than those of the control group. It is concluded that the simulated microgravity can affect the secreting and assembly of the extracellular matrix. The possible mechanism for them is discussed.
