Summary： This study aimed to identify biochemical predictors of adverse perinatal outcomes in in- trahepatic cholestasis of pregnancy （ICP）. A total of 106 ICP cases were analyzed retrospectively by the combination...Summary： This study aimed to identify biochemical predictors of adverse perinatal outcomes in in- trahepatic cholestasis of pregnancy （ICP）. A total of 106 ICP cases were analyzed retrospectively by the combination of receiver operating characteristic curve and binary logistic regression analysis. ＂Adverse perinatal outcomes＂ included spontaneous preterm labor, meconium-staining of amniotic fluid, stillbirth and Apgar score ≤7 at 1 or 5 min. Total bile acid （TBA） [AUC=0.658, 95%CI （0.536, 0.781）, P=0.031] was a valuable predictor for adverse perinatal outcomes. The critical value of TBA above which adverse perinatal outcomes were observed was 40.15 μmol/L （Youden＇s index=0.3）. Binary multivariate logistic regression analysis revealed that the risk of adverse perinatal outcomes increased when TBA ≥40.15 /.tmol/L [OR=3.792, 95%CI （1.226, 11.727）, P=0.021]. It is concluded that the risk of adverse perinatal outcomes in ICP increases when maternal TBA ≥40.15 gmol/L.展开更多
This study examined the effect of cholic acid （CA） on cultured cardiac myoeytes （CMs） from neonatal rats with an attempt to explore the possible mechanism of sudden fetal death in intra- hepatic cholestasis of pre...This study examined the effect of cholic acid （CA） on cultured cardiac myoeytes （CMs） from neonatal rats with an attempt to explore the possible mechanism of sudden fetal death in intra- hepatic cholestasis of pregnancy （ICP）. Inverted microscopy was performed to detect the impact of CA on the beating rates of rat CMs. MTT method was used to study the effect of CA on the viability of CMs. CMs cultured in vitro were incubated with 10 ~maol/L Ca2＋-sensitive fluorescence indicator fluo-3/AM. The fluorescence signals of free calcium induced by CA were measured under a laser scanning confocal microscope. The results showed that CA decreased the beating rates of the CMs in a dose-dependent manner. CA could suppress the activities of CMs in a time- and dose-dependent manner. CA increased the concentration of intracellular free calcium in a dose-dependent manner. Our study suggested that CA could inhibit the activity of CMs by causing calcium overload, thereby leading to the sudden fetal death in ICP.展开更多
目的探讨胃液总胆汁酸(total bile acid,TBA)和幽门螺旋杆菌(Helicobacter pylori,Hp)感染与胃癌的关系。方法选择胃镜下诊断为胃癌的患者60例为研究组,病理检查诊断为腺癌;选择同期健康体检人群胃镜提示慢性非萎缩性胃炎患者60例为对照...目的探讨胃液总胆汁酸(total bile acid,TBA)和幽门螺旋杆菌(Helicobacter pylori,Hp)感染与胃癌的关系。方法选择胃镜下诊断为胃癌的患者60例为研究组,病理检查诊断为腺癌;选择同期健康体检人群胃镜提示慢性非萎缩性胃炎患者60例为对照组,病理提示无腺体萎缩、异型增生、肠化生。抽取患者的胃液标准检测TBA浓度,对组织标本行免疫组化染色检测尾型同源盒2(caudal type homeobox 2,CDX2)、黏蛋白2(mucin 2,MUC2)、黏蛋白5AC(mucin 5AC,MUC5AC)的表达,采用快速尿素酶试验和组织学改良Giemsa染色检测Hp。结果研究组的TBA浓度高于对照组,Hp阳性感染率高于对照组(P<0.05)。研究组CDX2阳性表达32例,占比53.33%,MUC2阳性表达33例,占比55.00%,MUC5AC阳性表达25例,占比41.67%;对照组CDX2、MUC2均无阳性表达,MUC5AC阳性表达52例,占比86.67%。研究组的CDX2和MUC2的阳性表达率高于对照组,MUC5AC阳性表达率低于对照组(P<0.05)。研究组Hp阳性者的CDX2和MUC2的阳性表达率高于Hp阴性者,MUC5AC阳性表达率低于Hp阴性者(P<0.05)。Hp阳性和胃液TBA浓度≥300μmol/L是CDX2、MUC2阳性表达的危险因素(P<0.05),是MUC5AC阳性表达的保护因素(P<0.05)。结论胃液TBA浓度≥300μmol/L和Hp阳性是CDX2、MUC2阳性表达的危险因素,是MUC5AC阳性表达的保护因素。胃液TBA和Hp可能通过影响CDX2、MUC2、MUC5AC的表达而影响胃癌的发生。展开更多
基金supported by grants from the National Natural Science Foundation of China (Nos. 30973205,81172464)
文摘Summary： This study aimed to identify biochemical predictors of adverse perinatal outcomes in in- trahepatic cholestasis of pregnancy （ICP）. A total of 106 ICP cases were analyzed retrospectively by the combination of receiver operating characteristic curve and binary logistic regression analysis. ＂Adverse perinatal outcomes＂ included spontaneous preterm labor, meconium-staining of amniotic fluid, stillbirth and Apgar score ≤7 at 1 or 5 min. Total bile acid （TBA） [AUC=0.658, 95%CI （0.536, 0.781）, P=0.031] was a valuable predictor for adverse perinatal outcomes. The critical value of TBA above which adverse perinatal outcomes were observed was 40.15 μmol/L （Youden＇s index=0.3）. Binary multivariate logistic regression analysis revealed that the risk of adverse perinatal outcomes increased when TBA ≥40.15 /.tmol/L [OR=3.792, 95%CI （1.226, 11.727）, P=0.021]. It is concluded that the risk of adverse perinatal outcomes in ICP increases when maternal TBA ≥40.15 gmol/L.
基金supported by a grant from Union Hospital,Tongji Medical College,Huazhong University of Science and Technology(No.Union-Grant 02.03.2012-03)
文摘This study examined the effect of cholic acid （CA） on cultured cardiac myoeytes （CMs） from neonatal rats with an attempt to explore the possible mechanism of sudden fetal death in intra- hepatic cholestasis of pregnancy （ICP）. Inverted microscopy was performed to detect the impact of CA on the beating rates of rat CMs. MTT method was used to study the effect of CA on the viability of CMs. CMs cultured in vitro were incubated with 10 ~maol/L Ca2＋-sensitive fluorescence indicator fluo-3/AM. The fluorescence signals of free calcium induced by CA were measured under a laser scanning confocal microscope. The results showed that CA decreased the beating rates of the CMs in a dose-dependent manner. CA could suppress the activities of CMs in a time- and dose-dependent manner. CA increased the concentration of intracellular free calcium in a dose-dependent manner. Our study suggested that CA could inhibit the activity of CMs by causing calcium overload, thereby leading to the sudden fetal death in ICP.
文摘目的探讨胃液总胆汁酸(total bile acid,TBA)和幽门螺旋杆菌(Helicobacter pylori,Hp)感染与胃癌的关系。方法选择胃镜下诊断为胃癌的患者60例为研究组,病理检查诊断为腺癌;选择同期健康体检人群胃镜提示慢性非萎缩性胃炎患者60例为对照组,病理提示无腺体萎缩、异型增生、肠化生。抽取患者的胃液标准检测TBA浓度,对组织标本行免疫组化染色检测尾型同源盒2(caudal type homeobox 2,CDX2)、黏蛋白2(mucin 2,MUC2)、黏蛋白5AC(mucin 5AC,MUC5AC)的表达,采用快速尿素酶试验和组织学改良Giemsa染色检测Hp。结果研究组的TBA浓度高于对照组,Hp阳性感染率高于对照组(P<0.05)。研究组CDX2阳性表达32例,占比53.33%,MUC2阳性表达33例,占比55.00%,MUC5AC阳性表达25例,占比41.67%;对照组CDX2、MUC2均无阳性表达,MUC5AC阳性表达52例,占比86.67%。研究组的CDX2和MUC2的阳性表达率高于对照组,MUC5AC阳性表达率低于对照组(P<0.05)。研究组Hp阳性者的CDX2和MUC2的阳性表达率高于Hp阴性者,MUC5AC阳性表达率低于Hp阴性者(P<0.05)。Hp阳性和胃液TBA浓度≥300μmol/L是CDX2、MUC2阳性表达的危险因素(P<0.05),是MUC5AC阳性表达的保护因素(P<0.05)。结论胃液TBA浓度≥300μmol/L和Hp阳性是CDX2、MUC2阳性表达的危险因素,是MUC5AC阳性表达的保护因素。胃液TBA和Hp可能通过影响CDX2、MUC2、MUC5AC的表达而影响胃癌的发生。
