WRKY is a plant-specific transcription factor(TF) involved in the regulation of many biological processes; however, its role in leaf senescence of leafy vegetables remains unknown. In the present work, a WRKY TF, term...WRKY is a plant-specific transcription factor(TF) involved in the regulation of many biological processes; however, its role in leaf senescence of leafy vegetables remains unknown. In the present work, a WRKY TF, termed Br WRKY75 was isolated from Chinese flowering cabbage [Brassica rapa L. ssp. chinensis(L.) Mokino var. utilis Tsen et Lee]. Analysis of deduced amino acid sequence and the phylogenetic tree showed that Br WRKY75 has high homology with WRKY75 from Brassica oleracea and Arabidopsis thaliana, and belongs to the II c sub-group. Sub-cellular localization and transcriptional activity analysis revealed that Br WRKY75 is a nuclear protein with transcriptional repression activity, and was up-regulated during leaf senescence. Electrophoretic mobility shift assay confirmed that Br WRKY75 directly bound to the W-box(TTGAC) cis-element. Collectively,these results provide a basis for further investigation of the transcriptional regulation of Chinese flowering cabbage leaf senescence.展开更多
【目的】考察施用γ-聚谷氨酸(γ-PGA)对菜心生长、产量、品质和养分吸收的影响,揭示γ-PGA的农学施用效应。【方法】在酸性土壤(p H 5.89)和强酸性土壤(p H 4.85)以菜心为供试作物进行盆栽试验。在常规施肥(施N 1.33 g/kg土,分5次施入...【目的】考察施用γ-聚谷氨酸(γ-PGA)对菜心生长、产量、品质和养分吸收的影响,揭示γ-PGA的农学施用效应。【方法】在酸性土壤(p H 5.89)和强酸性土壤(p H 4.85)以菜心为供试作物进行盆栽试验。在常规施肥(施N 1.33 g/kg土,分5次施入土壤)的基础上,设不施γ-PGA包裹型肥料(T1)、分5次施用γ-PGA 0.53mg/kg土(T2)和1.06 mg/kg(T3)、在定苗一周后一次性淋施γ-PGA 0.53 mg/kg土(T4)和1.06 mg/kg土(T5)5个处理。调查了菜心根系活力、养分含量、养分吸收累积量、叶片抗氧化系统、菜心产量及品质。【结果】酸性、强酸性土壤上,γ-PGA处理(T2、T3、T4、T5)的伤流强度均高于T1,且T3、T4、T5的伤流液氨基酸输出强度显著高于T1,平均增幅分别达到38.1%、33.2%、44.2%(P<0.05)。酸性土壤上,T2、T3、T4、T5处理的菜心钙、镁含量显著高于T1(P<0.05),菜心钙的增幅分别为29.6%、21.8%、26.4%、22.7%,镁的增幅分别为11.7%、8.3%、22.2%、16.7%,锌、锰也有一定程度的提高;强酸性土壤上不同处理的菜心养分含量没有差异。T2、T3、T4、T5处理的氮、磷、钾、钙、镁、铜、锌、铁、锰累积量均显著高于T1;T4、T5处理的菜心叶片可溶性蛋白显著高于T1(P<0.05),T2、T3、T4、T5处理的菜心叶片CAT活性显著高于T1(P<0.05);而酸性土壤上,仅T3处理的菜心叶片CAT活性显著高于T1处理(P<0.05)。酸性、强酸性土壤上,T3、T4处理的菜心叶片SOD活性显著高于T1(P<0.05);不同处理的菜心叶片POD活性没有差异;T2、T3、T4、T5处理的菜心产量均显著高于T1(P<0.05),在酸性土壤上增幅分别为4.4%、7.3%、12.2%、12.3%,强酸性土壤上分别为9.7%、14.2%、12.2%、12.39%。两种类型土壤上,T2、T3、T4、T5处理的菜心地上部分和根系生物量均显著高于T1处理(P<0.05)。T4、T5处理的Vc含量和游离氨基酸含量显著高于T1(P<0.05),T2、T3、T4、T5处理的硝酸盐含量显著低于T1(P<0.05)。γ-PGA淋施处�展开更多
基金supported by the ‘The Twelfth Five-Year Plan’ National Science and Technology Support Program (2013BAD19B07)
文摘WRKY is a plant-specific transcription factor(TF) involved in the regulation of many biological processes; however, its role in leaf senescence of leafy vegetables remains unknown. In the present work, a WRKY TF, termed Br WRKY75 was isolated from Chinese flowering cabbage [Brassica rapa L. ssp. chinensis(L.) Mokino var. utilis Tsen et Lee]. Analysis of deduced amino acid sequence and the phylogenetic tree showed that Br WRKY75 has high homology with WRKY75 from Brassica oleracea and Arabidopsis thaliana, and belongs to the II c sub-group. Sub-cellular localization and transcriptional activity analysis revealed that Br WRKY75 is a nuclear protein with transcriptional repression activity, and was up-regulated during leaf senescence. Electrophoretic mobility shift assay confirmed that Br WRKY75 directly bound to the W-box(TTGAC) cis-element. Collectively,these results provide a basis for further investigation of the transcriptional regulation of Chinese flowering cabbage leaf senescence.
