Objective：To investigate effect of Chaiqin Chengqi Decoction（柴芩承气汤,CQCQD） on changes of neuronal acetylcholine receptor alpha 7（nAChRα7） of peritoneal macrophages in acute pancreatitis（AP）.Methods：Eighte...Objective：To investigate effect of Chaiqin Chengqi Decoction（柴芩承气汤,CQCQD） on changes of neuronal acetylcholine receptor alpha 7（nAChRα7） of peritoneal macrophages in acute pancreatitis（AP）.Methods：Eighteen Kunming mice were equally randomized into the control group,AP group and CQCQD treatment group.AP was induced by two intraperitoneal injections of 4 g/kg L-arginine at 1 h apart,while control mice received saline injections.At 72 h after the first injection of L-arginine,mice in the treatment group were intragastrically administered 0.1 mL/10 g CQCQD every 2 h for 3 times,whilst mice in the other two groups received the same amount of saline feeding.Mice were sacrificed by cervical dislocation 2 h after the last feeding of either CQCQD or saline.Peritoneal macrophages were collected for determination of nAChRα7 mRNA and protein expression.Serum was collected for detection of interleukin-6（IL-6）,IL-10 and acetylcholine（ACh）levels,and pancreas was for histopathology analysis.Results：The CQCQD treatment significantly ameliorated the severity of AP as evidenced by reducing the pancreatic histopathology score（4.5 ± 0.5 vs.6.2 ± 1.7,P〈0.05）and the serum IL-6 levels（1228.31419.2 pg/mL vs.1589.6 ±337.3 pg/mL,P〈0.05）.The mRNA and protein expression of nAChRα7 of the peritoneal macrophages in the AP group were similar to the control group（P〉0.05）,but were significantly up-regulated after the CQCQD treatment（P〈0.05）.The serum ACh levels in the AP group were significantly lower than those in the control group（3.1 ± 0.6 μg/mL vs 4.8 ± 0.7 μg/mL P〈0.05）,but were significantly increased after the CQCQD treatment（5.6±1.5 μg/mL vs 3.1 ±0.6 μg/mL,P〈0.05）.Conclusion：CQCQD is protective against L-arginine-induced AP through mechanisms involving nAChR α 7 of peritoneal macrophages.展开更多
Objective： To investigate the effect of Chaiqin Chengqi Decoction （柴芩承气汤, CQCQD） on regulating serum matrix metalloproteinase 9 （MMP-9） in patients with severe acute pancreatitis （SAP）. Methods： Thirty-fi...Objective： To investigate the effect of Chaiqin Chengqi Decoction （柴芩承气汤, CQCQD） on regulating serum matrix metalloproteinase 9 （MMP-9） in patients with severe acute pancreatitis （SAP）. Methods： Thirty-five SAP patients hospitalized in West China Hospital from September 1, 2008 to February 28, 2009 were randomly assigned to two groups using a computer-derived random number sequence in a ratio of 1：1, treatment group （18 patients） and the placebo control group （17 patients）. The patients in the treatment group were administered with CQCQD by gastric parfusion （50 mL/2 h） and retention enema （200 mL/6 h） for 7 days. The two groups had similar baseline information. The clinical indicators, including the initial Balthazar＇s computed tomography （CT） score, acute physiology and chronic health evaluation I1 （APACHE II） scores on 1st, 3rd, 5th and 7th day, incidences and durations of complications and the serum C-reactive protein （CRP）, levels of MMP-9 on the 1st, 3rd, 5th and 7th day, were recorded and compared between the two groups. Resalts： The serum MMP-9, CRP and the APACHE I1 scores on the 3rd, 5th and 7th day in the treatment group were lower than those in the control group （P〈0.05）. The serum MMP-9 was positively correlated with the APACHE II score on the 1st day （r=0.430, P=0.01）. The durations of acute respiratory distress syndrome （5.4± 2.4 vs. 2.9± 1.3）, acute hepatitis （4.6± 0.8 vs. 1.9 ± 0.6） and acute heart failure （3.9 ± 1.6 vs. 1.3 ± 0.6, P〈0.05） in the control group were longer than those in the treatment group. Conclusion： CQCQD could decrease the serum MMP-9 to relieve the severity of clinical symptoms and prevent the development of multiple organ dysfunction syndrome in patients with SAP.展开更多
OBJECTIVE: To investigate the effect of Chaiqin Chengqi decoction(CQCQD) on acute pancreatitis(AP) by janus kinase/signal transducer and activator of transcription(JAK/STAT) signaling pathway in vitro and in vivo.METH...OBJECTIVE: To investigate the effect of Chaiqin Chengqi decoction(CQCQD) on acute pancreatitis(AP) by janus kinase/signal transducer and activator of transcription(JAK/STAT) signaling pathway in vitro and in vivo.METHODS: AP was induced by caerulein both in AR42J cells and in mice. AR42J cells were divided into five groups: the control group, the AP group, the CQCQD group, JAK/STAT signaling pathway inhibitor AG490 group, and the CQCQD and AG490 group.After induction, cellular supernatant of five groups were collected for measuring the concentrations of inflammatory cytokine amylase, interleukin 6(IL-6),tumor necrosis factor α(TNF-α), interleukin 1β(IL-1β), nuclear factor κB(NF-κB) by enzyme-linked immunosorbent assay and the expression of JAK-2,STAT-3 signaling transduction proteins by Western blot, respectively. Experiments in mice were performed similar to that of in AR42J cells.RESULTS: Treatment of AR42J cells with CQCQD reduced the pancreatic injury and negatively regulated the activities of amylase, as well as inhibited expression of several inflammatory cytokines such as IL-6, TNF-α, IL-1β, NF-κB. Administration of CQCQD significantly inhibited JAK-2 activation and down-regulated phosphorylation of downstream substrate STAT-3 the same as AG490, resulting in inhibition of inflammatory mediators and amelioration of pancreatitis.CONCLUSION: The results suggested that CQCQD exerted anti-inflammatory effects on AP via reducing expression and phosphorylation of JAK and STAT.展开更多
目的系统评价柴芩承气汤联合常规西医治疗急性胰腺炎的有效性和安全性。方法计算机检索发表在PubMed、Embase、Cochrane Library、Web of Science、中国知网(CNKI)、万方(WanFang)、中国生物医学文献数据库(CBM)、维普(VIP)数据库上的...目的系统评价柴芩承气汤联合常规西医治疗急性胰腺炎的有效性和安全性。方法计算机检索发表在PubMed、Embase、Cochrane Library、Web of Science、中国知网(CNKI)、万方(WanFang)、中国生物医学文献数据库(CBM)、维普(VIP)数据库上的相关文献,检索时限为建库至2020年12月。根据纳入排除标准进行文献筛选和数据提取。采用Cochrane偏倚风险评估表评价文献质量,运用RevMan 5.4软件进行Meta分析。结果共纳入11项研究,共994例患者。Meta分析结果显示,试验组能够提高治疗总有效率,降低血清淀粉酶水平,减少住院时间、缩短腹痛缓解时间,且各方面均优于对照组,在不良反应发生率方面差异无统计学意义。结论柴芩承气汤联合西医常规治疗急性胰腺炎优于单纯西药疗法,但不良反应需进一步评价。展开更多
Objective： To investigate the effect of Chaiqin Chengqi Decoction （柴芩承气汤,CQCQD） on cholecystokinin receptor 1 （CCKR1）-mediated signal transduction of pancreatic acinar cell in rats with acute necrotic pancre...Objective： To investigate the effect of Chaiqin Chengqi Decoction （柴芩承气汤,CQCQD） on cholecystokinin receptor 1 （CCKR1）-mediated signal transduction of pancreatic acinar cell in rats with acute necrotic pancreatitis （ANP）. Methods： Twenty-seven Sprague-Dawley rats were randomized into three groups： the control group, the ANP group, and the CQCQD group （9 in each group）. ANP rats were induced by two intraperitoneal injections of 8% L-arginine （pH=7.0, 4.4 g/kg） over a 2-h period. Rats were treated with 1.5 mL/100 g body weight of CQCQD （CQCQD group） or physiological saline （control and ANP groups） at 2 h interval. And 6 h after induction, pancreatic tissues were collected for histopathological examination. Pancreatic acinar cells were isolated for determination of CCKR1 mRNA and protein expression, phospholipase C （PLC） and inositol-1,4,5-triphosphate （IP3）, and determination of fluorescence intensity （FI） as a measure of intracellular calcium ion concentration [Ca2＋]i. Results： The pancreatic histopathological score （6.2± 1.1） and the levels of PLC （1,187.2±228.2μg/mL） and IP3 （872.2±88.4 μg/mL） of acinar cells in the ANP group were higher than those in the control （2.8± 0.4, 682.5± 121.8 μ g/mL, 518.4 ± 115.8 μ g/mL） and the CQCQD （3.8± 0.8, 905.3± 78.5 μ g/mL, 611.0±42.5μ g/mL） groups （P〈0.05）. [Ca2＋]i FI for the ANP group （34.8± 27.0） was higher than that in the control （5.1 ± 2.2） and CQCQD （12.6± 2.5） groups （P〈0.05）. The expression of pancreatic acinar cell CCKR1 mRNA in the ANP group was up-regulated （expression ratio=1.761; ,0=0.024） compared with the control group. The expression of pancreatic acinar cell CCKR1 mRNA in the CQCQD group was down-regulated （expression ratio=0.311; P=0.035） compared with the ANP group. The ratio of gray values of the CCKR1 and β-actin in the ANP group （1.43±0.17） was higher than those in the control （0.70 ± 0.15） and CQCQD （0.79± 0.1展开更多
基金Supported by National Natural Science Foundation of China(No.81072910)Science and Technology Support Program of Sichuan(No.2011SZ0291,2010SZ0068)
文摘Objective：To investigate effect of Chaiqin Chengqi Decoction（柴芩承气汤,CQCQD） on changes of neuronal acetylcholine receptor alpha 7（nAChRα7） of peritoneal macrophages in acute pancreatitis（AP）.Methods：Eighteen Kunming mice were equally randomized into the control group,AP group and CQCQD treatment group.AP was induced by two intraperitoneal injections of 4 g/kg L-arginine at 1 h apart,while control mice received saline injections.At 72 h after the first injection of L-arginine,mice in the treatment group were intragastrically administered 0.1 mL/10 g CQCQD every 2 h for 3 times,whilst mice in the other two groups received the same amount of saline feeding.Mice were sacrificed by cervical dislocation 2 h after the last feeding of either CQCQD or saline.Peritoneal macrophages were collected for determination of nAChRα7 mRNA and protein expression.Serum was collected for detection of interleukin-6（IL-6）,IL-10 and acetylcholine（ACh）levels,and pancreas was for histopathology analysis.Results：The CQCQD treatment significantly ameliorated the severity of AP as evidenced by reducing the pancreatic histopathology score（4.5 ± 0.5 vs.6.2 ± 1.7,P〈0.05）and the serum IL-6 levels（1228.31419.2 pg/mL vs.1589.6 ±337.3 pg/mL,P〈0.05）.The mRNA and protein expression of nAChRα7 of the peritoneal macrophages in the AP group were similar to the control group（P〉0.05）,but were significantly up-regulated after the CQCQD treatment（P〈0.05）.The serum ACh levels in the AP group were significantly lower than those in the control group（3.1 ± 0.6 μg/mL vs 4.8 ± 0.7 μg/mL P〈0.05）,but were significantly increased after the CQCQD treatment（5.6±1.5 μg/mL vs 3.1 ±0.6 μg/mL,P〈0.05）.Conclusion：CQCQD is protective against L-arginine-induced AP through mechanisms involving nAChR α 7 of peritoneal macrophages.
基金Supported by Science and Technology Support Program of Sichuan(No.2011SZ0291,2010SZ0068)
文摘Objective： To investigate the effect of Chaiqin Chengqi Decoction （柴芩承气汤, CQCQD） on regulating serum matrix metalloproteinase 9 （MMP-9） in patients with severe acute pancreatitis （SAP）. Methods： Thirty-five SAP patients hospitalized in West China Hospital from September 1, 2008 to February 28, 2009 were randomly assigned to two groups using a computer-derived random number sequence in a ratio of 1：1, treatment group （18 patients） and the placebo control group （17 patients）. The patients in the treatment group were administered with CQCQD by gastric parfusion （50 mL/2 h） and retention enema （200 mL/6 h） for 7 days. The two groups had similar baseline information. The clinical indicators, including the initial Balthazar＇s computed tomography （CT） score, acute physiology and chronic health evaluation I1 （APACHE II） scores on 1st, 3rd, 5th and 7th day, incidences and durations of complications and the serum C-reactive protein （CRP）, levels of MMP-9 on the 1st, 3rd, 5th and 7th day, were recorded and compared between the two groups. Resalts： The serum MMP-9, CRP and the APACHE I1 scores on the 3rd, 5th and 7th day in the treatment group were lower than those in the control group （P〈0.05）. The serum MMP-9 was positively correlated with the APACHE II score on the 1st day （r=0.430, P=0.01）. The durations of acute respiratory distress syndrome （5.4± 2.4 vs. 2.9± 1.3）, acute hepatitis （4.6± 0.8 vs. 1.9 ± 0.6） and acute heart failure （3.9 ± 1.6 vs. 1.3 ± 0.6, P〈0.05） in the control group were longer than those in the treatment group. Conclusion： CQCQD could decrease the serum MMP-9 to relieve the severity of clinical symptoms and prevent the development of multiple organ dysfunction syndrome in patients with SAP.
基金Supported by the National S&T Major Project of China,Construction of Technical Platform for New Preparation and New Release Drug System in Compliance with GLP Requirements(No.2012ZX09304004001)
文摘OBJECTIVE: To investigate the effect of Chaiqin Chengqi decoction(CQCQD) on acute pancreatitis(AP) by janus kinase/signal transducer and activator of transcription(JAK/STAT) signaling pathway in vitro and in vivo.METHODS: AP was induced by caerulein both in AR42J cells and in mice. AR42J cells were divided into five groups: the control group, the AP group, the CQCQD group, JAK/STAT signaling pathway inhibitor AG490 group, and the CQCQD and AG490 group.After induction, cellular supernatant of five groups were collected for measuring the concentrations of inflammatory cytokine amylase, interleukin 6(IL-6),tumor necrosis factor α(TNF-α), interleukin 1β(IL-1β), nuclear factor κB(NF-κB) by enzyme-linked immunosorbent assay and the expression of JAK-2,STAT-3 signaling transduction proteins by Western blot, respectively. Experiments in mice were performed similar to that of in AR42J cells.RESULTS: Treatment of AR42J cells with CQCQD reduced the pancreatic injury and negatively regulated the activities of amylase, as well as inhibited expression of several inflammatory cytokines such as IL-6, TNF-α, IL-1β, NF-κB. Administration of CQCQD significantly inhibited JAK-2 activation and down-regulated phosphorylation of downstream substrate STAT-3 the same as AG490, resulting in inhibition of inflammatory mediators and amelioration of pancreatitis.CONCLUSION: The results suggested that CQCQD exerted anti-inflammatory effects on AP via reducing expression and phosphorylation of JAK and STAT.
文摘目的系统评价柴芩承气汤联合常规西医治疗急性胰腺炎的有效性和安全性。方法计算机检索发表在PubMed、Embase、Cochrane Library、Web of Science、中国知网(CNKI)、万方(WanFang)、中国生物医学文献数据库(CBM)、维普(VIP)数据库上的相关文献,检索时限为建库至2020年12月。根据纳入排除标准进行文献筛选和数据提取。采用Cochrane偏倚风险评估表评价文献质量,运用RevMan 5.4软件进行Meta分析。结果共纳入11项研究,共994例患者。Meta分析结果显示,试验组能够提高治疗总有效率,降低血清淀粉酶水平,减少住院时间、缩短腹痛缓解时间,且各方面均优于对照组,在不良反应发生率方面差异无统计学意义。结论柴芩承气汤联合西医常规治疗急性胰腺炎优于单纯西药疗法,但不良反应需进一步评价。
基金Supported by the National Natural Science Foundation of China(No.81072910)the Science and Technology Supported Program of Sichuan Province,China(No.2010SZ0068 and 2011SZ0291)
文摘Objective： To investigate the effect of Chaiqin Chengqi Decoction （柴芩承气汤,CQCQD） on cholecystokinin receptor 1 （CCKR1）-mediated signal transduction of pancreatic acinar cell in rats with acute necrotic pancreatitis （ANP）. Methods： Twenty-seven Sprague-Dawley rats were randomized into three groups： the control group, the ANP group, and the CQCQD group （9 in each group）. ANP rats were induced by two intraperitoneal injections of 8% L-arginine （pH=7.0, 4.4 g/kg） over a 2-h period. Rats were treated with 1.5 mL/100 g body weight of CQCQD （CQCQD group） or physiological saline （control and ANP groups） at 2 h interval. And 6 h after induction, pancreatic tissues were collected for histopathological examination. Pancreatic acinar cells were isolated for determination of CCKR1 mRNA and protein expression, phospholipase C （PLC） and inositol-1,4,5-triphosphate （IP3）, and determination of fluorescence intensity （FI） as a measure of intracellular calcium ion concentration [Ca2＋]i. Results： The pancreatic histopathological score （6.2± 1.1） and the levels of PLC （1,187.2±228.2μg/mL） and IP3 （872.2±88.4 μg/mL） of acinar cells in the ANP group were higher than those in the control （2.8± 0.4, 682.5± 121.8 μ g/mL, 518.4 ± 115.8 μ g/mL） and the CQCQD （3.8± 0.8, 905.3± 78.5 μ g/mL, 611.0±42.5μ g/mL） groups （P〈0.05）. [Ca2＋]i FI for the ANP group （34.8± 27.0） was higher than that in the control （5.1 ± 2.2） and CQCQD （12.6± 2.5） groups （P〈0.05）. The expression of pancreatic acinar cell CCKR1 mRNA in the ANP group was up-regulated （expression ratio=1.761; ,0=0.024） compared with the control group. The expression of pancreatic acinar cell CCKR1 mRNA in the CQCQD group was down-regulated （expression ratio=0.311; P=0.035） compared with the ANP group. The ratio of gray values of the CCKR1 and β-actin in the ANP group （1.43±0.17） was higher than those in the control （0.70 ± 0.15） and CQCQD （0.79± 0.1
