Background Human papillomavirus （HPV） is believed to be the most common sexually transmitted infection. However,little is known about the prevalence and distribution of HPV types in China. We aimed to assess the pre...Background Human papillomavirus （HPV） is believed to be the most common sexually transmitted infection. However,little is known about the prevalence and distribution of HPV types in China. We aimed to assess the prevalence and the distribution of HPV types as well as risks for abnormal cervical cytology in women who reside in the Tibetan Autonomous Region of China.Methods A cross-sectional study was performed involving a sample of 3036 women. An epidemiological questionnaire was applied and cervical specimens were obtained for liquid-based cytology and HPV DNA detection. Statistical analysis included chi-square and Logistic regression model.Results In this population, 3.66% （111/3036） had atypical squamous cells of undetermined significance （ASCUS）,1.45% （44/3036） low-grade squamous intraepithelial lesions （LSIL） and 1.09% （33/3036） had high-grade squamous intraepithelial lesions （HSIL）. Tibetan women （5.74%, 137/2387） exhibited lower abnormal cytology rates than non-Tibetan women （8.01%, 52/649, P=0.03）. The overall prevalence of HPV infection was 9.19% （279/3036）. We failed to identify any differences in HPV prevalence by age. In the groups with normal, ASCUS, LSIL and HSIL, the overall HPV prevalences were 7.41% （211/2847）, 24.32% （27/111）, 56.82% （25/44） and 45.45% （15/33）, respectively. HPV 16 （1.52%,46/3036） was the most common type, and was also the most prevalent in women with ASCUS （8.11%, 9/111） and HSIL （15.15%, 5/33）. The most common HPV type for Tibetan women was HPV 16 （1.42%, 34/2387）, whereas for non-Tibetan individuals it was HPV 33 （2.31%, 15/649）. Of the 279 HPV-infected women, 40 individuals （14.34%） presented with multiple HPV positivity. Women who had two pregnancies were more likely to have abnormal cytology smear （OR=1.67;95% CI： 1.07-2.61）.Conclusions A low prevalence of HPV positivity was observed in women who reside in the Tibetan Autonomous Region of China. The prevalence of abnormal cervical cytology展开更多
Background It was reported that telomerase expression is closely associated with cellular immortality and cancer. This study was designed to investigate the relationship between telomerase expression and the carcinoge...Background It was reported that telomerase expression is closely associated with cellular immortality and cancer. This study was designed to investigate the relationship between telomerase expression and the carcinogenesis of cervical cancer, the possible use of telomerase as a marker of cervical intraepithelial neoplasia (CIN) progression or regression, and the natural history of CIN.Methods Telomeric repeat amplification protocol (TRAP) assay was used to measure telomerase activity in cervical scrapings and biopsy samples obtained from 105 cases affected with various cervical conditions, including chronic cervicitis (n =20), CIN (n =64, 16 cases of CIN Ⅰ , 20 cases of CIN Ⅱ , and 28 cases of CIN Ⅲ), and invasive squamous cell carcinoma (n =21).Results In exfoliated cell samples, telomerase activity was detected in 5 of 20 (25. 0%) cases of cervicitis, 10 of 16 (62. 5%) cases of CIN Ⅰ , 11 of 20 (55. 0%) cases of CIN Ⅱ , 23 of 28 (82.1%) cases of CIN Ⅲ, and 13 of 21 (61. 9%) cases of carcinoma. In cervical biopsy samples, telomerase activity was detected in 6 of 20 (30. 0%) cases of cervicitis, 8 of 16 (50. 0%) cases of CIN Ⅰ , 9 of 20 (45. 0%) cases of CIN Ⅱ , 27 of 28 (96. 4%) cases of CIN Ⅲ, and 20 of 21 (95. 2%) cases of carcinoma. Telomerase activation was significantly higher in CIN samples than in cervicitis samples. Telomerase activity was detected at similar frequency in samples from cervical scrapings and cervical biopsies.Conclusion These results seem to suggest that telomerase expression may be associated with carcinogenesis of the cervix. TRAP assay of cervical scraping samples could be used to monitor and predict the development of CIN in clinical practice.展开更多
文摘Background Human papillomavirus （HPV） is believed to be the most common sexually transmitted infection. However,little is known about the prevalence and distribution of HPV types in China. We aimed to assess the prevalence and the distribution of HPV types as well as risks for abnormal cervical cytology in women who reside in the Tibetan Autonomous Region of China.Methods A cross-sectional study was performed involving a sample of 3036 women. An epidemiological questionnaire was applied and cervical specimens were obtained for liquid-based cytology and HPV DNA detection. Statistical analysis included chi-square and Logistic regression model.Results In this population, 3.66% （111/3036） had atypical squamous cells of undetermined significance （ASCUS）,1.45% （44/3036） low-grade squamous intraepithelial lesions （LSIL） and 1.09% （33/3036） had high-grade squamous intraepithelial lesions （HSIL）. Tibetan women （5.74%, 137/2387） exhibited lower abnormal cytology rates than non-Tibetan women （8.01%, 52/649, P=0.03）. The overall prevalence of HPV infection was 9.19% （279/3036）. We failed to identify any differences in HPV prevalence by age. In the groups with normal, ASCUS, LSIL and HSIL, the overall HPV prevalences were 7.41% （211/2847）, 24.32% （27/111）, 56.82% （25/44） and 45.45% （15/33）, respectively. HPV 16 （1.52%,46/3036） was the most common type, and was also the most prevalent in women with ASCUS （8.11%, 9/111） and HSIL （15.15%, 5/33）. The most common HPV type for Tibetan women was HPV 16 （1.42%, 34/2387）, whereas for non-Tibetan individuals it was HPV 33 （2.31%, 15/649）. Of the 279 HPV-infected women, 40 individuals （14.34%） presented with multiple HPV positivity. Women who had two pregnancies were more likely to have abnormal cytology smear （OR=1.67;95% CI： 1.07-2.61）.Conclusions A low prevalence of HPV positivity was observed in women who reside in the Tibetan Autonomous Region of China. The prevalence of abnormal cervical cytology
文摘Background It was reported that telomerase expression is closely associated with cellular immortality and cancer. This study was designed to investigate the relationship between telomerase expression and the carcinogenesis of cervical cancer, the possible use of telomerase as a marker of cervical intraepithelial neoplasia (CIN) progression or regression, and the natural history of CIN.Methods Telomeric repeat amplification protocol (TRAP) assay was used to measure telomerase activity in cervical scrapings and biopsy samples obtained from 105 cases affected with various cervical conditions, including chronic cervicitis (n =20), CIN (n =64, 16 cases of CIN Ⅰ , 20 cases of CIN Ⅱ , and 28 cases of CIN Ⅲ), and invasive squamous cell carcinoma (n =21).Results In exfoliated cell samples, telomerase activity was detected in 5 of 20 (25. 0%) cases of cervicitis, 10 of 16 (62. 5%) cases of CIN Ⅰ , 11 of 20 (55. 0%) cases of CIN Ⅱ , 23 of 28 (82.1%) cases of CIN Ⅲ, and 13 of 21 (61. 9%) cases of carcinoma. In cervical biopsy samples, telomerase activity was detected in 6 of 20 (30. 0%) cases of cervicitis, 8 of 16 (50. 0%) cases of CIN Ⅰ , 9 of 20 (45. 0%) cases of CIN Ⅱ , 27 of 28 (96. 4%) cases of CIN Ⅲ, and 20 of 21 (95. 2%) cases of carcinoma. Telomerase activation was significantly higher in CIN samples than in cervicitis samples. Telomerase activity was detected at similar frequency in samples from cervical scrapings and cervical biopsies.Conclusion These results seem to suggest that telomerase expression may be associated with carcinogenesis of the cervix. TRAP assay of cervical scraping samples could be used to monitor and predict the development of CIN in clinical practice.
