目的探讨电刺激小脑顶核对大鼠局灶脑缺血再灌注后脑保护作用的机制。方法 Sprague-Dawley大鼠分为正常对照组(NC组)、缺血再灌注组(I/R组)、缺血再灌注后小脑顶核刺激组(FNS组)、毁损小脑顶核组(FNL组),后3组根据再灌注时间分为7 d和1...目的探讨电刺激小脑顶核对大鼠局灶脑缺血再灌注后脑保护作用的机制。方法 Sprague-Dawley大鼠分为正常对照组(NC组)、缺血再灌注组(I/R组)、缺血再灌注后小脑顶核刺激组(FNS组)、毁损小脑顶核组(FNL组),后3组根据再灌注时间分为7 d和14 d两个亚组,每个亚组6只。大脑中动脉线栓法制作缺血再灌注模型。相应时间点采用Western blotting检测脑梗死周围组织核因子-κB(NF-κB)P50蛋白表达,逆转录-聚合酶链反应法(RT-PCR)检测肿瘤坏死因子-α(TNF-α)和Bcl-x L m RNA表达,同时检测各组脑梗死体积。结果与I/R组比较,FNS组NF-κB P50蛋白表达各时间点均增高(P<0.05),TNF-αm RNA表达明显降低(P<0.01),Bcl-x L m RNA表达升高(P<0.05),梗死面积明显减少(P<0.01)。与I/R组比较,FNL组上述指标均无显著性差异(P>0.05)。结论FNS可有效提高脑缺血再灌注后NF-κB P50蛋白、Bcl-x L m RNA表达,抑制下游炎症因子TNF-αm RNA的表达,减小脑梗死体积,可能是FNS发挥中枢神经保护的机制之一。展开更多
The effects of stimulating locus coeruleus (LC) on neuronal activity of cerebellar fastigial nucleus (FN) was investigated. Stimulation of LC elicited inhibitory, excitatory and biphasic (inhibition-excitation) respon...The effects of stimulating locus coeruleus (LC) on neuronal activity of cerebellar fastigial nucleus (FN) was investigated. Stimulation of LC elicited inhibitory, excitatory and biphasic (inhibition-excitation) responses from FN cells. The majority of responsive cells showed an inhibitory response with a latency of less than 10 ms. Injection of α adrenoreceptor antagonists phentolamine (ⅳ) could block the inhibitory response of FN cells to the LC stimulation, but propranolol (ⅳ), a β adrenoreceptor antagonist, could not. These results suggest that LC-cerebellar noradrenergic afferent fibers may be involved in the cerebellar sensorimotor integration process by exerting their modulatory action on the cerebellar nuclear cells’ activities.展开更多
基金supported by the National Natural Science Foundation of China(No.30670671)the Educational Science Research Foundation of Jiangsu Province(No.09KJD310009)the Research Foundation of Xuzhou Medical college
文摘目的探讨电刺激小脑顶核对大鼠局灶脑缺血再灌注后脑保护作用的机制。方法 Sprague-Dawley大鼠分为正常对照组(NC组)、缺血再灌注组(I/R组)、缺血再灌注后小脑顶核刺激组(FNS组)、毁损小脑顶核组(FNL组),后3组根据再灌注时间分为7 d和14 d两个亚组,每个亚组6只。大脑中动脉线栓法制作缺血再灌注模型。相应时间点采用Western blotting检测脑梗死周围组织核因子-κB(NF-κB)P50蛋白表达,逆转录-聚合酶链反应法(RT-PCR)检测肿瘤坏死因子-α(TNF-α)和Bcl-x L m RNA表达,同时检测各组脑梗死体积。结果与I/R组比较,FNS组NF-κB P50蛋白表达各时间点均增高(P<0.05),TNF-αm RNA表达明显降低(P<0.01),Bcl-x L m RNA表达升高(P<0.05),梗死面积明显减少(P<0.01)。与I/R组比较,FNL组上述指标均无显著性差异(P>0.05)。结论FNS可有效提高脑缺血再灌注后NF-κB P50蛋白、Bcl-x L m RNA表达,抑制下游炎症因子TNF-αm RNA的表达,减小脑梗死体积,可能是FNS发挥中枢神经保护的机制之一。
文摘The effects of stimulating locus coeruleus (LC) on neuronal activity of cerebellar fastigial nucleus (FN) was investigated. Stimulation of LC elicited inhibitory, excitatory and biphasic (inhibition-excitation) responses from FN cells. The majority of responsive cells showed an inhibitory response with a latency of less than 10 ms. Injection of α adrenoreceptor antagonists phentolamine (ⅳ) could block the inhibitory response of FN cells to the LC stimulation, but propranolol (ⅳ), a β adrenoreceptor antagonist, could not. These results suggest that LC-cerebellar noradrenergic afferent fibers may be involved in the cerebellar sensorimotor integration process by exerting their modulatory action on the cerebellar nuclear cells’ activities.
