研究了在 Ca2 + 存在下 ,丹磺酰标记的花粉钙调素 ( D-p Ca M)与合成的多肽相互作用的规律 .研究结果表明 ,被研究的绝大多数多肽能与 D-p Ca M结合而形成复合物 ,使 D-p Ca M的荧光光谱发生变化 .用荧光法测定了这些复合物的解离常数 ...研究了在 Ca2 + 存在下 ,丹磺酰标记的花粉钙调素 ( D-p Ca M)与合成的多肽相互作用的规律 .研究结果表明 ,被研究的绝大多数多肽能与 D-p Ca M结合而形成复合物 ,使 D-p Ca M的荧光光谱发生变化 .用荧光法测定了这些复合物的解离常数 Kd,其中肽 BP-1 3的 Kd 值为 4 .0 nmol/ L,是与 p Ca M结合能力最强的一个多肽 .除证明了多肽的疏水性 ,预测二级结构形成倾向和链长对其与 p Ca M结合能力有影响外 ,还发现用D-丙氨酸取代肽链中的 Gly或 L-丙氨酸后 ,可改变肽对 p Ca M的亲和性 ,这为提高多肽与钙调素的亲和力提供了一条改进的途径 .我们的研究还可以定性地说明不同来源 Ca M性质相似又有差异 ,反映了它们的高度保守性和变异性 .同时还展示了所研究的多肽对细胞信息传导过程的影响 ,为研究 Ca M对细胞功能调节的作用机制和建立 p Ca展开更多
Chronic heart failure(CHF ) occurs when abnormality of cardiac function causes the heart to fail to pump bloodat a rate required by the metabolising tissue. CHF is highly complex disorders that arise as a result of co...Chronic heart failure(CHF ) occurs when abnormality of cardiac function causes the heart to fail to pump bloodat a rate required by the metabolising tissue. CHF is highly complex disorders that arise as a result of combination of genetic,pathophysiologic, and environmental factors. Cellular transduction plays an important role in the pathogenesis of CHF andmany signaling molecules, rebeptors, and intracellular signaling paths are involved. Peptide hormones, growth factors, andcytokines can activate heteromeric (Gq ) or low-molecular-weight guanosine triphosphate (GTP) -binding protein (ras ), andthe transmembrane signal transducer gp130 and induce CHF respectively. Recently it is reported that calcineurin (a Ca2+/calmodulin dependent phosphoprotein phosphatase) plays a significant role in myocyte hypertrophy and heart failure. Thepresent article reviews the progress in cellular signal transduction mechanisms in CHF.展开更多
The interaction between dansyl-labeled pollen calmodulin (D-pCaM) and synthesized peptides was studied in the presence of Ca2+ by fluorescence spectra. It is Found that Gly/L-Ala --> D-Ala substitution in peptide c...The interaction between dansyl-labeled pollen calmodulin (D-pCaM) and synthesized peptides was studied in the presence of Ca2+ by fluorescence spectra. It is Found that Gly/L-Ala --> D-Ala substitution in peptide chains caused great changes in their affinity for pCaM. Besides. our data provided evidence on the dissimilarity of different CaMs although they have highly-conserved structures. A preliminary study was carried out on the effects of CaM-binding peptides on cellular signal transduction, cell proliferation, showing the participation of CaM in cell functions mentioned above.展开更多
文摘研究了在 Ca2 + 存在下 ,丹磺酰标记的花粉钙调素 ( D-p Ca M)与合成的多肽相互作用的规律 .研究结果表明 ,被研究的绝大多数多肽能与 D-p Ca M结合而形成复合物 ,使 D-p Ca M的荧光光谱发生变化 .用荧光法测定了这些复合物的解离常数 Kd,其中肽 BP-1 3的 Kd 值为 4 .0 nmol/ L,是与 p Ca M结合能力最强的一个多肽 .除证明了多肽的疏水性 ,预测二级结构形成倾向和链长对其与 p Ca M结合能力有影响外 ,还发现用D-丙氨酸取代肽链中的 Gly或 L-丙氨酸后 ,可改变肽对 p Ca M的亲和性 ,这为提高多肽与钙调素的亲和力提供了一条改进的途径 .我们的研究还可以定性地说明不同来源 Ca M性质相似又有差异 ,反映了它们的高度保守性和变异性 .同时还展示了所研究的多肽对细胞信息传导过程的影响 ,为研究 Ca M对细胞功能调节的作用机制和建立 p Ca
文摘Chronic heart failure(CHF ) occurs when abnormality of cardiac function causes the heart to fail to pump bloodat a rate required by the metabolising tissue. CHF is highly complex disorders that arise as a result of combination of genetic,pathophysiologic, and environmental factors. Cellular transduction plays an important role in the pathogenesis of CHF andmany signaling molecules, rebeptors, and intracellular signaling paths are involved. Peptide hormones, growth factors, andcytokines can activate heteromeric (Gq ) or low-molecular-weight guanosine triphosphate (GTP) -binding protein (ras ), andthe transmembrane signal transducer gp130 and induce CHF respectively. Recently it is reported that calcineurin (a Ca2+/calmodulin dependent phosphoprotein phosphatase) plays a significant role in myocyte hypertrophy and heart failure. Thepresent article reviews the progress in cellular signal transduction mechanisms in CHF.
文摘The interaction between dansyl-labeled pollen calmodulin (D-pCaM) and synthesized peptides was studied in the presence of Ca2+ by fluorescence spectra. It is Found that Gly/L-Ala --> D-Ala substitution in peptide chains caused great changes in their affinity for pCaM. Besides. our data provided evidence on the dissimilarity of different CaMs although they have highly-conserved structures. A preliminary study was carried out on the effects of CaM-binding peptides on cellular signal transduction, cell proliferation, showing the participation of CaM in cell functions mentioned above.
