3D-printed Porous Titanium Alloy Implants(pTi),owing to their biologically inertness and relatively smooth surface morphology,adversely affect the biological functions of surrounding cells.To address the challenges,co...3D-printed Porous Titanium Alloy Implants(pTi),owing to their biologically inertness and relatively smooth surface morphology,adversely affect the biological functions of surrounding cells.To address the challenges,constructing a bioinspired interface that mimics the hierarchical structure of bone tissue can enhance the cellular functions of cells.In this context,Hollow Mesoporous Silica Nanoparticles(HMSNs),renowned for their unique physicochemical properties and superior biocompatibility,offer a promising direction for this research.In this research,the initially synthesized HMSNs were used to construct a“hollow-mesoporous-macroporous”hierarchical bioinspired coating on the pTi surface through the Layer-by-Layer technique.Simultaneously,diverse morphologies of coatings were established by adjusting the deposition strategy of PDDA/HMSNs on the pTi surface(pTi-HMSN-2,pTi-HMSN-4,pTi-HMSN-6).A range of techniques were employed to investigate the physicochemical properties and regulation of cellular biological functions of the diverse HMSN coating strategies.Notably,the pTi-HMSN-4 and pTi-HMSN-6 groups exhibited the uniform coatings,leading to a substantial enhancement in surface roughness and hydrophilicity.Meantime,the coating constructed strategy of pTi-HMSN-4 possessed commendable stability.Based on the aforementioned findings,both pTi-HMSN-4 and pTi-HMSN-6 facilitated the adhesion,spreading,and pseudopodia extension of BMSCs,which led to a notable upsurge in the expression levels of vinculin protein in BMSCs.Comprehensive analysis indicates that the coating,when PDDA/HMSNs are deposited four times,possesses favorable overall performance.The research will provide a solid theoretical basis for the translation of HMSN bioinspired coatings for orthopedic implants.展开更多
We identify the functions whose polynomial multiples are weak＊ dense in Qp spaces and prove that if ｜f（z）｜ ≥ ｜g（z）｜ and g is cyclic in Qp, then f is cyclic in Qp. We also show that the multiplication operato...We identify the functions whose polynomial multiples are weak＊ dense in Qp spaces and prove that if ｜f（z）｜ ≥ ｜g（z）｜ and g is cyclic in Qp, then f is cyclic in Qp. We also show that the multiplication operator Mx on Qp spaces is cellular indecomposable.展开更多
目的探讨人T细胞免疫球蛋白与黏蛋白1(Tim-1)在胶质母细胞瘤中的表达及对肿瘤细胞生物学功能的影响。方法通过组织芯片免疫组织化学、蛋白质印迹法(Western blot)检测Tim-1在胶质母细胞瘤组织(西安百思达生物科技有限公司,GL805a、GL80...目的探讨人T细胞免疫球蛋白与黏蛋白1(Tim-1)在胶质母细胞瘤中的表达及对肿瘤细胞生物学功能的影响。方法通过组织芯片免疫组织化学、蛋白质印迹法(Western blot)检测Tim-1在胶质母细胞瘤组织(西安百思达生物科技有限公司,GL805a、GL805c)及细胞株(购自中国科学院上海细胞研究所)中的表达;通过RNA干扰(RNAi)技术构建下调Tim-1表达的胶质母细胞瘤细胞株,采用t检验比较Tim-1敲低组和Tim-1对照组细胞在增殖、侵袭、迁移上的差异。结果Tim-1在胶质母细胞瘤组织中的表达(142.769±37.552)高于正常脑组织(43.501±23.811);在胶质母细胞瘤细胞株(U87、U251)中的表达高于人脑正常胶质细胞株(HEB)(HEB:0.339±0.021,U87:0.919±0.066,t=21.910,P<0.01;U251:1.074±0.085,t=12.559,P<0.01);成功构建下调Tim-1的胶质母细胞瘤细胞株(sh-Tim-1-U87、sh-Tim-1-U251);细胞计数试剂盒(CCK-8)结果显示下调Tim-1后肿瘤细胞活力显著降低(24 h sh-NC:0.379±0.059,sh-U87:0.223±0.046,t=2.767,P<0.05;sh-U251:0.210±0.036,t=5.637,P<0.01。48 h sh-NC:0.677±0.034,sh-U87:0.444±0.052,t=4.968,P<0.01;sh-U251:0.441±0.056,t=8.831,P<0.01。72 h sh-NC:0.801±0.052,sh-U87:0.579±0.060,t=4.487,P<0.01;sh-U251:0.636±0.056,t=8.991,P<0.01);划痕实验结果表明下调Tim-1后肿瘤细胞迁移能力显著降低(sh-NC:74.667±4.510,sh-U87:44.333±4.509,t=11.651,P<0.01;sh-U251:34.003±3.606,t=18.401,P<0.01);Transwell实验显示下调Tim-1后肿瘤细胞侵袭能力显著降低(sh-NC:202.000±10.149,sh-U87:108.333±7.506,t=11.294,P<0.01;sh-U251:137.667±11.061,t=12.763,P<0.01)。结论Tim-1在胶母细胞瘤组织和细胞株中高表达,下调Tim-1可有效抑制胶质母细胞瘤细胞的增殖、迁移和侵袭能力。展开更多
Good’s buffers have been widely applied in cell/organ culture over the past half a century as biocompatible pH stabilizers.However,the emergence of severe adverse effects,such as cellular uptake,lysosomal autophagic ...Good’s buffers have been widely applied in cell/organ culture over the past half a century as biocompatible pH stabilizers.However,the emergence of severe adverse effects,such as cellular uptake,lysosomal autophagic activation,and visible light-induced cytotoxicity,raises serious questions over its biocompatibility while underlying mechanism was unclear.Here we report that riboflavin(RF,component of cell culture medium)generates ^(1)O_(2),⋅OH,and O_(2)^(·-)under visible light exposure during regular cell manipulation.These short half-life reactive oxygen species(ROS)react with tertiary amine groups of HEPES,producing 106.6μM of H_(2)O_(2).Orders of magnitude elevated half-life of ROS in the medium caused severe cytotoxicity and systematic disorder of normal cell functions.We have further designed and validated zwitterionic betaines as the new generation biocompatible organic pH buffers,which is able to completely avoid the adverse effects that found on HEPES and derivate Good’s buffers.These findings may also open a new avenue for zwitterionic betaine based materials for biomedical applications.展开更多
Melanoma is one of the most dangerous types of cutaneous neoplasms,which are pigment-producing cells of neuroectodermal origin found all over the body.A great deal of research is focused on the mechanisms of melanoma ...Melanoma is one of the most dangerous types of cutaneous neoplasms,which are pigment-producing cells of neuroectodermal origin found all over the body.A great deal of research is focused on the mechanisms of melanoma to promote better diagnostic and treatment options for melanoma in its advanced stages.The progression of melanoma involves alteration in different levels of gene expression.With the successful implementation of next-generation sequencing technology,an increasing number of long noncoding RNAs(lncRNAs)sequences have been discovered,and a significant number of them have phenotypic effects in both in vitro and in vivo studies,implying that they play an important role in the occurrence and progression of human cancers,particularly melanoma.A number of evidence indicated that lncRNAs are important regulators in tumor cell proliferation,invasion,apoptosis,immune escape,energy metabolism,drug resistance,epigenetic regulation.To better understand the role of lncRNAs in melanoma tumorigenesis,we categorize melanomaassociated lncRNAs according to their cellular functions and associations with gene expression and signaling pathways in this review.Based on the mechanisms of lncRNA,we discuss the possibility of lncRNA-target treatments,and the application of liquid biopsies to detect lncRNAs in melanomadiagnosisandprognosis.展开更多
This review discusses the various regulatory charac-teristics of microRNAs that are capable of generating widespread changes in gene expression via post translational repression of many mRNA targets and control self-r...This review discusses the various regulatory charac-teristics of microRNAs that are capable of generating widespread changes in gene expression via post translational repression of many mRNA targets and control self-renewal, differentiation and division of cells. It controls the stem cell functions by controlling a wide range of pathological and physiological processes, including development, differentiation, cellular proliferation, programmed cell death, oncogenesis and metastasis. Through either mRNA cleavage or translational repression, miRNAs alter the expression of their cognate target genes; thereby modulating cellular pathways that affect the normal functions of stem cells, turning them into cancer stem cells, a likely cause of relapse in cancer patients. This present review further emphasizes the recent discoveries on the functional analysis of miRNAs in cancer metastasis and implications on miRNA based therapy using miRNA replacement or anti-miRNA technologies in specific cancer stem cells that are required to establish their efficacy in controlling tumorigenic potential and safe therapeutics.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.82372391,82001971,82102358,82202698,52105343,U21A2099 and U23A20523)Project of“Medical+X”interdisciplinary innovation team of Norman Bethune Health Science Center of Jilin University(Grant No.2022JBGS06)+5 种基金Project of youth interdisciplinary innovation team of Jilin University(Grant No.419070623054)China Postdoctoral Science Foundation(Grant No.2021M701384)Bethune Plan of Jilin University(Grant No.2022B27,2022B03)Wu Jieping Medical Foundation(Grant No.320.6750.18522)Scientific Development Program of Jilin Province(Grant No.20220402067GH)Jilin Province Development and Reform Commission(Grant No.2022C044-2).
文摘3D-printed Porous Titanium Alloy Implants(pTi),owing to their biologically inertness and relatively smooth surface morphology,adversely affect the biological functions of surrounding cells.To address the challenges,constructing a bioinspired interface that mimics the hierarchical structure of bone tissue can enhance the cellular functions of cells.In this context,Hollow Mesoporous Silica Nanoparticles(HMSNs),renowned for their unique physicochemical properties and superior biocompatibility,offer a promising direction for this research.In this research,the initially synthesized HMSNs were used to construct a“hollow-mesoporous-macroporous”hierarchical bioinspired coating on the pTi surface through the Layer-by-Layer technique.Simultaneously,diverse morphologies of coatings were established by adjusting the deposition strategy of PDDA/HMSNs on the pTi surface(pTi-HMSN-2,pTi-HMSN-4,pTi-HMSN-6).A range of techniques were employed to investigate the physicochemical properties and regulation of cellular biological functions of the diverse HMSN coating strategies.Notably,the pTi-HMSN-4 and pTi-HMSN-6 groups exhibited the uniform coatings,leading to a substantial enhancement in surface roughness and hydrophilicity.Meantime,the coating constructed strategy of pTi-HMSN-4 possessed commendable stability.Based on the aforementioned findings,both pTi-HMSN-4 and pTi-HMSN-6 facilitated the adhesion,spreading,and pseudopodia extension of BMSCs,which led to a notable upsurge in the expression levels of vinculin protein in BMSCs.Comprehensive analysis indicates that the coating,when PDDA/HMSNs are deposited four times,possesses favorable overall performance.The research will provide a solid theoretical basis for the translation of HMSN bioinspired coatings for orthopedic implants.
基金supported by NNSF of China (10771130)Specialized Research Fund for the Doctoral Program of High Education (2007056004)+1 种基金NSF of GuangdongProvince (10151503101000025)NSF of Fujian Province (2009J01004)
文摘We identify the functions whose polynomial multiples are weak＊ dense in Qp spaces and prove that if ｜f（z）｜ ≥ ｜g（z）｜ and g is cyclic in Qp, then f is cyclic in Qp. We also show that the multiplication operator Mx on Qp spaces is cellular indecomposable.
文摘目的探讨人T细胞免疫球蛋白与黏蛋白1(Tim-1)在胶质母细胞瘤中的表达及对肿瘤细胞生物学功能的影响。方法通过组织芯片免疫组织化学、蛋白质印迹法(Western blot)检测Tim-1在胶质母细胞瘤组织(西安百思达生物科技有限公司,GL805a、GL805c)及细胞株(购自中国科学院上海细胞研究所)中的表达;通过RNA干扰(RNAi)技术构建下调Tim-1表达的胶质母细胞瘤细胞株,采用t检验比较Tim-1敲低组和Tim-1对照组细胞在增殖、侵袭、迁移上的差异。结果Tim-1在胶质母细胞瘤组织中的表达(142.769±37.552)高于正常脑组织(43.501±23.811);在胶质母细胞瘤细胞株(U87、U251)中的表达高于人脑正常胶质细胞株(HEB)(HEB:0.339±0.021,U87:0.919±0.066,t=21.910,P<0.01;U251:1.074±0.085,t=12.559,P<0.01);成功构建下调Tim-1的胶质母细胞瘤细胞株(sh-Tim-1-U87、sh-Tim-1-U251);细胞计数试剂盒(CCK-8)结果显示下调Tim-1后肿瘤细胞活力显著降低(24 h sh-NC:0.379±0.059,sh-U87:0.223±0.046,t=2.767,P<0.05;sh-U251:0.210±0.036,t=5.637,P<0.01。48 h sh-NC:0.677±0.034,sh-U87:0.444±0.052,t=4.968,P<0.01;sh-U251:0.441±0.056,t=8.831,P<0.01。72 h sh-NC:0.801±0.052,sh-U87:0.579±0.060,t=4.487,P<0.01;sh-U251:0.636±0.056,t=8.991,P<0.01);划痕实验结果表明下调Tim-1后肿瘤细胞迁移能力显著降低(sh-NC:74.667±4.510,sh-U87:44.333±4.509,t=11.651,P<0.01;sh-U251:34.003±3.606,t=18.401,P<0.01);Transwell实验显示下调Tim-1后肿瘤细胞侵袭能力显著降低(sh-NC:202.000±10.149,sh-U87:108.333±7.506,t=11.294,P<0.01;sh-U251:137.667±11.061,t=12.763,P<0.01)。结论Tim-1在胶母细胞瘤组织和细胞株中高表达,下调Tim-1可有效抑制胶质母细胞瘤细胞的增殖、迁移和侵袭能力。
基金support from National Natural Science Foundation of China(No.21504046)Natural Science Foundation of Jiangsu Province(No.BK20150970)+1 种基金the Six Talent Peaks Project in Jiangsu Province(SWYY-060)the Projects of Nanjing Normal University(No.184080H20192,184080H10386).
文摘Good’s buffers have been widely applied in cell/organ culture over the past half a century as biocompatible pH stabilizers.However,the emergence of severe adverse effects,such as cellular uptake,lysosomal autophagic activation,and visible light-induced cytotoxicity,raises serious questions over its biocompatibility while underlying mechanism was unclear.Here we report that riboflavin(RF,component of cell culture medium)generates ^(1)O_(2),⋅OH,and O_(2)^(·-)under visible light exposure during regular cell manipulation.These short half-life reactive oxygen species(ROS)react with tertiary amine groups of HEPES,producing 106.6μM of H_(2)O_(2).Orders of magnitude elevated half-life of ROS in the medium caused severe cytotoxicity and systematic disorder of normal cell functions.We have further designed and validated zwitterionic betaines as the new generation biocompatible organic pH buffers,which is able to completely avoid the adverse effects that found on HEPES and derivate Good’s buffers.These findings may also open a new avenue for zwitterionic betaine based materials for biomedical applications.
基金This work was supported by the National Natural Science Foundation of China(No.81871578).
文摘Melanoma is one of the most dangerous types of cutaneous neoplasms,which are pigment-producing cells of neuroectodermal origin found all over the body.A great deal of research is focused on the mechanisms of melanoma to promote better diagnostic and treatment options for melanoma in its advanced stages.The progression of melanoma involves alteration in different levels of gene expression.With the successful implementation of next-generation sequencing technology,an increasing number of long noncoding RNAs(lncRNAs)sequences have been discovered,and a significant number of them have phenotypic effects in both in vitro and in vivo studies,implying that they play an important role in the occurrence and progression of human cancers,particularly melanoma.A number of evidence indicated that lncRNAs are important regulators in tumor cell proliferation,invasion,apoptosis,immune escape,energy metabolism,drug resistance,epigenetic regulation.To better understand the role of lncRNAs in melanoma tumorigenesis,we categorize melanomaassociated lncRNAs according to their cellular functions and associations with gene expression and signaling pathways in this review.Based on the mechanisms of lncRNA,we discuss the possibility of lncRNA-target treatments,and the application of liquid biopsies to detect lncRNAs in melanomadiagnosisandprognosis.
基金The Department of Science and Technology,Govt. of India for providing BOYSCAST fellowship 2011-2012
文摘This review discusses the various regulatory charac-teristics of microRNAs that are capable of generating widespread changes in gene expression via post translational repression of many mRNA targets and control self-renewal, differentiation and division of cells. It controls the stem cell functions by controlling a wide range of pathological and physiological processes, including development, differentiation, cellular proliferation, programmed cell death, oncogenesis and metastasis. Through either mRNA cleavage or translational repression, miRNAs alter the expression of their cognate target genes; thereby modulating cellular pathways that affect the normal functions of stem cells, turning them into cancer stem cells, a likely cause of relapse in cancer patients. This present review further emphasizes the recent discoveries on the functional analysis of miRNAs in cancer metastasis and implications on miRNA based therapy using miRNA replacement or anti-miRNA technologies in specific cancer stem cells that are required to establish their efficacy in controlling tumorigenic potential and safe therapeutics.
