The complex of Rubisco and Rubisco activase from LaCl3-, CeCl3- treated spinach in vivo is induced. SDS-PAGE result shows that the purified proteins from LaCl3-, CeCl3- treated spinach have not only large and small su...The complex of Rubisco and Rubisco activase from LaCl3-, CeCl3- treated spinach in vivo is induced. SDS-PAGE result shows that the purified proteins from LaCl3-, CeCl3- treated spinach have not only large and small subunits (55 kD, 14.4 kD) of Rubisco, but also two large subunits of 45 kD and 41 kD near the large subunits of Rubisco. Native-PAGE shows that the purified proteins from LaCl3-, CeCl3- treated spinach have not only a band of Rubisco (560 kD), but also a band of about 1100 kD, about twice distant from Rubisco, which might be a complex of Rubisco and Rubisco activase. The purified enzyme activities from LaCl3-, CeCl3-treated spinach are 1.8 and 2.8 times that of the control,the intensities of absorption and fluorescence are sig- nificantly higher than that of the purified Rubisco from the control, and the total sulfhydryl groups and available sulfhydryl groups are 36—39 –SH per mol enzyme, 14—25 –SH per mol enzyme more than those of the purified Rubisco from the control, respectively. The CD spectra show that the secondary structure of the purified enzyme from LaCl3-, CeCl3 -treated spinach is very dif- ferent from the control. The enzyme activities from LaCl3-, CeCl3- treated spinach in vivo are 1.5 and 1.9 times those of the control.展开更多
The sulfate ions modified YAG：Ce^（3＋） phosphors were prepared by co-precipitation method and characterized by X-ray diffraction,transmission electron microscopy,and photoluminescence.Effects of sulfate ions on the...The sulfate ions modified YAG：Ce^（3＋） phosphors were prepared by co-precipitation method and characterized by X-ray diffraction,transmission electron microscopy,and photoluminescence.Effects of sulfate ions on the photoluminescence（PL） property of the as-prepared YAG：Ce^（3＋） phosphors were studied,with sodium dodecyl sulfate（SDS） being added to R^（3＋）（Ce^（3＋）,Y^（3＋）,Al^（3＋）） ions.Results indicated that pure YAG：Ce^（3＋） phosphors with different ratios of sulfate ions could be easily obtained by calcining the as-synthesized precursor at 950 ℃ for 2 h,the YAG：Ce^（3＋） phosphors with an optimal mass ratio of 3.5 wt.%SDS had the highest emission intensity and the best dispersion behavior,and the fluorescence decay of the as-obtained YAG：Ce^（3＋） phosphors was related to the lattice defect,reabsorption and cross correlation.Furthermore,thermal quenching properties of the YAG：Ce^（3＋） phosphors and the YAG：Ce^（3＋） phosphors with 3.5 wt.%SDS were also discussed,indicating that the YAG：Ce^（3＋） with SDS phosphors could have potential applications in the daylight LEDs or warm white LEDs.展开更多
Ce3+/Eu2+ co-doped LiBaBO3 phosphor was synthesized by high temperature solid-state reaction method, and its luminescent character- istics were investigated. The hues of the LiBaBO3:Ce3+, Eu2+ phosphor varies from blu...Ce3+/Eu2+ co-doped LiBaBO3 phosphor was synthesized by high temperature solid-state reaction method, and its luminescent character- istics were investigated. The hues of the LiBaBO3:Ce3+, Eu2+ phosphor varies from blue to white and eventually to yellow-green by properly tuning the Ce3+/Eu2+ ratio. Under UV excitation, white light was generated by coupling blue and yellow-green emission bands attributed to Ce3+ and Eu2+ emissions, respectively. The luminous efficacy of LiBaBO3:1%Ce3+, 2%Eu2+ calculated from ...展开更多
It was studied by spectroscopy that PSII reaction center complex consisting of three polypeptides, D1, D2 and Cytb559, were purified from PSII particle of CeCl3 treated spinach. The results of the experiment show that...It was studied by spectroscopy that PSII reaction center complex consisting of three polypeptides, D1, D2 and Cytb559, were purified from PSII particle of CeCl3 treated spinach. The results of the experiment show that Ce3+ could improve the growth of spinach, and accelerate electron transport of PSII particles. Of chl-a of UV-Vis spectrum of D1/D2/Cytb559 complex, Soret band was blue-shifted by 3 nm and Q band by 2 nm, respectively, and the fluorescence emission peak was blue-shifted by 5 nm in CeCl3-treated spinach compared with the one in control. By the extended X-ray absorption fine structure (EXAFS) spectroscopy methods, it has been found that Ce3+ is coordinated with 8 nitrogen atoms in the first coordination shell with Ce-N bond length of 0.253 nm, and Ce3+ with 6 oxygen atoms in the second coordination shell with Ce-O bond length of 0.32 nm. However, the secondary structure of D1/D2/Cytb559 complex by circular dichroism (CD) spectroscopy has no significant change after CeCl3 treated. It might be that Ce3+ binds to porphyrin rings of chlorophyll and oxygen of amino acid residue of polypeptide in D1/D2/Cytb559 complex, and then accelerates the primary reaction of PSII, intensifies function of P680+ primary electron donor of D1/D2/Cytb559, but there is little change in conformation of PSII reaction center complex.展开更多
Ce3+,Eu3+ and Tb3+ singly doped and Ce3+/Eu3+ and Ce3+/Tb3+ co-doped zinc phosphate glasses were prepared by sintering P2O5,ZnO,Ce2(C2O4)3·10H2O and Eu2O3/Tb4O7 mixtures at 1200 °C in the air for 2 h and the...Ce3+,Eu3+ and Tb3+ singly doped and Ce3+/Eu3+ and Ce3+/Tb3+ co-doped zinc phosphate glasses were prepared by sintering P2O5,ZnO,Ce2(C2O4)3·10H2O and Eu2O3/Tb4O7 mixtures at 1200 °C in the air for 2 h and then annealing at 450 °C for 10 h.The obtained glasses were homogeneous and transparent.The glasses without Ce3+ were colorless and those with Ce3+ showed slightly yellow.The singly doped glasses showed strong emissions and excitations from doped trivalent rare earth ions.Strong energy transfer from Ce3+ to...展开更多
基金the National Natural Science Foundation of China(Grant No.30470150) the Natural Science Foundation of Jiangsu Province Universities(Grant No.03KJB180122).
文摘The complex of Rubisco and Rubisco activase from LaCl3-, CeCl3- treated spinach in vivo is induced. SDS-PAGE result shows that the purified proteins from LaCl3-, CeCl3- treated spinach have not only large and small subunits (55 kD, 14.4 kD) of Rubisco, but also two large subunits of 45 kD and 41 kD near the large subunits of Rubisco. Native-PAGE shows that the purified proteins from LaCl3-, CeCl3- treated spinach have not only a band of Rubisco (560 kD), but also a band of about 1100 kD, about twice distant from Rubisco, which might be a complex of Rubisco and Rubisco activase. The purified enzyme activities from LaCl3-, CeCl3-treated spinach are 1.8 and 2.8 times that of the control,the intensities of absorption and fluorescence are sig- nificantly higher than that of the purified Rubisco from the control, and the total sulfhydryl groups and available sulfhydryl groups are 36—39 –SH per mol enzyme, 14—25 –SH per mol enzyme more than those of the purified Rubisco from the control, respectively. The CD spectra show that the secondary structure of the purified enzyme from LaCl3-, CeCl3 -treated spinach is very dif- ferent from the control. The enzyme activities from LaCl3-, CeCl3- treated spinach in vivo are 1.5 and 1.9 times those of the control.
基金supported by the Research Fund for Doctoral Program of Higher Education of China(20120201130004)the Science and Technology Developing Project of Shaanxi Province(2015KW-001)+1 种基金the National Natural Science Foundation of China(61078058)Partially by the National Natural Science Foundation of China Major Research Plan on Nanomanufacturing(91323303)
文摘The sulfate ions modified YAG：Ce^（3＋） phosphors were prepared by co-precipitation method and characterized by X-ray diffraction,transmission electron microscopy,and photoluminescence.Effects of sulfate ions on the photoluminescence（PL） property of the as-prepared YAG：Ce^（3＋） phosphors were studied,with sodium dodecyl sulfate（SDS） being added to R^（3＋）（Ce^（3＋）,Y^（3＋）,Al^（3＋）） ions.Results indicated that pure YAG：Ce^（3＋） phosphors with different ratios of sulfate ions could be easily obtained by calcining the as-synthesized precursor at 950 ℃ for 2 h,the YAG：Ce^（3＋） phosphors with an optimal mass ratio of 3.5 wt.%SDS had the highest emission intensity and the best dispersion behavior,and the fluorescence decay of the as-obtained YAG：Ce^（3＋） phosphors was related to the lattice defect,reabsorption and cross correlation.Furthermore,thermal quenching properties of the YAG：Ce^（3＋） phosphors and the YAG：Ce^（3＋） phosphors with 3.5 wt.%SDS were also discussed,indicating that the YAG：Ce^（3＋） with SDS phosphors could have potential applications in the daylight LEDs or warm white LEDs.
基金Project supported by the National Natural Science Foundation of China (50902042)the Natural Science Foundation of Hebei Province, China (E2009000209, E2010000283)the Research Foundation of Education Bureau of Hebei Province, China (2009313)
文摘Ce3+/Eu2+ co-doped LiBaBO3 phosphor was synthesized by high temperature solid-state reaction method, and its luminescent character- istics were investigated. The hues of the LiBaBO3:Ce3+, Eu2+ phosphor varies from blue to white and eventually to yellow-green by properly tuning the Ce3+/Eu2+ ratio. Under UV excitation, white light was generated by coupling blue and yellow-green emission bands attributed to Ce3+ and Eu2+ emissions, respectively. The luminous efficacy of LiBaBO3:1%Ce3+, 2%Eu2+ calculated from ...
基金This work was supported by the National Natural Science Foundation of China (Grant No. 29805003) Person of Ability Recommended Foundation of Suzhou University (Grant No. XQ316011).
文摘It was studied by spectroscopy that PSII reaction center complex consisting of three polypeptides, D1, D2 and Cytb559, were purified from PSII particle of CeCl3 treated spinach. The results of the experiment show that Ce3+ could improve the growth of spinach, and accelerate electron transport of PSII particles. Of chl-a of UV-Vis spectrum of D1/D2/Cytb559 complex, Soret band was blue-shifted by 3 nm and Q band by 2 nm, respectively, and the fluorescence emission peak was blue-shifted by 5 nm in CeCl3-treated spinach compared with the one in control. By the extended X-ray absorption fine structure (EXAFS) spectroscopy methods, it has been found that Ce3+ is coordinated with 8 nitrogen atoms in the first coordination shell with Ce-N bond length of 0.253 nm, and Ce3+ with 6 oxygen atoms in the second coordination shell with Ce-O bond length of 0.32 nm. However, the secondary structure of D1/D2/Cytb559 complex by circular dichroism (CD) spectroscopy has no significant change after CeCl3 treated. It might be that Ce3+ binds to porphyrin rings of chlorophyll and oxygen of amino acid residue of polypeptide in D1/D2/Cytb559 complex, and then accelerates the primary reaction of PSII, intensifies function of P680+ primary electron donor of D1/D2/Cytb559, but there is little change in conformation of PSII reaction center complex.
基金supported by the National Natural Science Foundation of China (10704090, 10874253)Foundation of the Education Committee of Chongqing (10874173)
文摘Ce3+,Eu3+ and Tb3+ singly doped and Ce3+/Eu3+ and Ce3+/Tb3+ co-doped zinc phosphate glasses were prepared by sintering P2O5,ZnO,Ce2(C2O4)3·10H2O and Eu2O3/Tb4O7 mixtures at 1200 °C in the air for 2 h and then annealing at 450 °C for 10 h.The obtained glasses were homogeneous and transparent.The glasses without Ce3+ were colorless and those with Ce3+ showed slightly yellow.The singly doped glasses showed strong emissions and excitations from doped trivalent rare earth ions.Strong energy transfer from Ce3+ to...
