This article thinks the original author of dream of the red chamber is Cao Shuo,Cao Xueqin’s second elder uncle,The manuscript reached the highest level of the world literature works after adding and deleting by Cao ...This article thinks the original author of dream of the red chamber is Cao Shuo,Cao Xueqin’s second elder uncle,The manuscript reached the highest level of the world literature works after adding and deleting by Cao xueqin,so Cao was the more greater author,he was the son of Cao Fu,born in the middle of May,1723,died in the spring of 1764,three people were the commentators:"Zhi YanZhai"was CaoYan,Cao xueqin’s third elder uncle,"JiHuSou"was Cao Fu,CaoXueQin’s father,"Song Zhai","MeiXi","Chang Cun"all was Cao Tangcun Cao Xueqin’s younger brother.展开更多
利用网络药理学方法预测加味甘草附子汤(modified Gan Cao Fu Zi Decoction, GCFZ)治疗类风湿关节炎(rheumatoid arthritis, RA)的作用机制,通过动物实验验证分析结果并探讨GCFZ的药效学效果。首先利用TCMID、SymMap、HERB、STITCH和GE...利用网络药理学方法预测加味甘草附子汤(modified Gan Cao Fu Zi Decoction, GCFZ)治疗类风湿关节炎(rheumatoid arthritis, RA)的作用机制,通过动物实验验证分析结果并探讨GCFZ的药效学效果。首先利用TCMID、SymMap、HERB、STITCH和GEO数据库获取GCFZ治疗RA的靶基因,共筛选得到RA差异表达基因1 250个, GCFZ靶点基因534个,交集基因83个。随后通过GO和KEGG数据库对交集基因进行功能富集分析,发现GCFZ及其活性成分主要通过细胞因子通路发挥作用,其中趋化因子信号通路、肿瘤坏死因子(tumor necrosis factor, TNF)信号通路富集基因数较多。接着使用Cytoscape3.8.0软件构建药物-靶点-疾病网络并筛选到TNF、趋化因子8 (C-X-C chemokine ligand 8, CXCL8)、趋化因子10 (C-X-C chemokine ligand 10, CXCL10)、趋化因子配体5(C-Cchemokineligand5,CCL5)、趋化因子2(C-X-Cchemokineligand2,CXCL2)、趋化因子受体4(C-X-C chemokine receptor type 4, CXCR4)等6个核心蛋白,并通过分子对接技术验证GCFZ主要活性成分与核心蛋白结合能力。通过构建胶原诱导性关节炎(collagen-induced arthritis, CIA)大鼠模型探讨GCFZ低、中、高(4、8、16 g·kg^(-1))剂量组的治疗效果, X射线影像学方法、HE染色、番红O-固绿染色结果显示, GCFZ干预能显著改善CIA大鼠骨质破坏、滑膜增生和软骨损伤,免疫荧光结果显示GCFZ治疗能够调节TNF、CXCL8、CCL5的表达。综上,本研究结果表明GCFZ含有多种小分子药效物质,能够通过多靶点、多通路发挥治疗作用,明显减轻CIA大鼠的关节炎症状。本研究动物实验获得蚌埠医学院实验动物管理和伦理委员会批准。展开更多
文摘This article thinks the original author of dream of the red chamber is Cao Shuo,Cao Xueqin’s second elder uncle,The manuscript reached the highest level of the world literature works after adding and deleting by Cao xueqin,so Cao was the more greater author,he was the son of Cao Fu,born in the middle of May,1723,died in the spring of 1764,three people were the commentators:"Zhi YanZhai"was CaoYan,Cao xueqin’s third elder uncle,"JiHuSou"was Cao Fu,CaoXueQin’s father,"Song Zhai","MeiXi","Chang Cun"all was Cao Tangcun Cao Xueqin’s younger brother.
