This paper deals with tubeseedling afforestation of Calamus simplicifolius. The survival rate of tubeseedlings planted in arboretum and Duadanga grandiflora forest is not lower than that of normal seedling...This paper deals with tubeseedling afforestation of Calamus simplicifolius. The survival rate of tubeseedlings planted in arboretum and Duadanga grandiflora forest is not lower than that of normal seedlings. That indicates the afforestation requirements of tubeseedlings, such as site selection, site preparation, shading trees and planting, can be similar to those of normal seedlings. The survival rate of tubeseedlings planted in arboretum, teak forest, Michelia macclurei forest, Duadanga grandiflora forest and natural secondary forest can be higher than ninety percent. The afforestation results of clump seedlings show that the survival rate of shoots in the clump is relative to their initial height before afforestation. The highest shoot in clumps has the highest survival rate. In clump seedlings, individual shoots with a height lower than 20 cm have very low afforestation survival rate. There is eminent difference among the height growth of different tissue culture families. This indicates the selection of elite family or plus plant can enhance the production of rattan forests. Experiment results also show that plants developed from tubeseedlings have stronger suckering ability and can germinate suckers one or two years earlier than those developed from normal seedlings. This phenomenon needs more research and more attention should be paid on the remnant impact of plant growth regulators applied in proliferation culture.展开更多
The growth character of 4 tube seedling families, namely, CS3, CS5, CS10 and CS11 of Calamus simplicifolius was analyzed through the comparitive experiment with normal seedlings(CK). The result showed that at the age ...The growth character of 4 tube seedling families, namely, CS3, CS5, CS10 and CS11 of Calamus simplicifolius was analyzed through the comparitive experiment with normal seedlings(CK). The result showed that at the age of 1, the seedling’ height of CS3 and CS5 was higher than that of both CS10 and CS11, gaining highly significant difference while the height of CS10 and CS11 was higher than that of CK at significant and extremely significant level respectively. After afforestation, the survival rate of 4 families decreased rapidly at first, and then decreasing gradually slow down, finally, the rate kept steady with year increasing. Survival rate varied a bit, but no significant difference was found among families. Tissue culture families started to sucker more identically and earlier than normal seedlings. As for suckering rate and mean number of sucker shoot, tissue culture families were higher than normal seedlings, and then the sequence of suckering ability, CS11>CS10>CS5>CS3 was arranged. The total stem number of the clump was consistent with suckering ability among families where CS11 was the largest, CS10 and CS5 was larger, and CS3 as well as CK was the smallest, but no significant difference appeared. Mean length of mother stem and mean total length of rattan clump differed significantly at the level of 0.05 and 0.01 among families. Based on the variance analysis and multiple comparisons, two types of tissue culture families, superior type and common one were classified.展开更多
The random amplified polymorphic DNA （RAPD） molecular marker technique was used to determine the sex of Calamus simplicifolius C. F. Wei In the present study, DNA samples were extracted individually from 10 male and...The random amplified polymorphic DNA （RAPD） molecular marker technique was used to determine the sex of Calamus simplicifolius C. F. Wei In the present study, DNA samples were extracted individually from 10 male and 10 female plants. After a total of 1 040 decamer primers had been tested, an approximate 500-bp male-specific DNA fragment was generated with the S 1443 primer. It is feasible to identify sex at the early stages of plant life, which is beneficial for improving breeding programs of this dioecious species. In addition, we have obtained a proper RAPD protocol that is useful for other species of rattan.展开更多
文摘This paper deals with tubeseedling afforestation of Calamus simplicifolius. The survival rate of tubeseedlings planted in arboretum and Duadanga grandiflora forest is not lower than that of normal seedlings. That indicates the afforestation requirements of tubeseedlings, such as site selection, site preparation, shading trees and planting, can be similar to those of normal seedlings. The survival rate of tubeseedlings planted in arboretum, teak forest, Michelia macclurei forest, Duadanga grandiflora forest and natural secondary forest can be higher than ninety percent. The afforestation results of clump seedlings show that the survival rate of shoots in the clump is relative to their initial height before afforestation. The highest shoot in clumps has the highest survival rate. In clump seedlings, individual shoots with a height lower than 20 cm have very low afforestation survival rate. There is eminent difference among the height growth of different tissue culture families. This indicates the selection of elite family or plus plant can enhance the production of rattan forests. Experiment results also show that plants developed from tubeseedlings have stronger suckering ability and can germinate suckers one or two years earlier than those developed from normal seedlings. This phenomenon needs more research and more attention should be paid on the remnant impact of plant growth regulators applied in proliferation culture.
文摘The growth character of 4 tube seedling families, namely, CS3, CS5, CS10 and CS11 of Calamus simplicifolius was analyzed through the comparitive experiment with normal seedlings(CK). The result showed that at the age of 1, the seedling’ height of CS3 and CS5 was higher than that of both CS10 and CS11, gaining highly significant difference while the height of CS10 and CS11 was higher than that of CK at significant and extremely significant level respectively. After afforestation, the survival rate of 4 families decreased rapidly at first, and then decreasing gradually slow down, finally, the rate kept steady with year increasing. Survival rate varied a bit, but no significant difference was found among families. Tissue culture families started to sucker more identically and earlier than normal seedlings. As for suckering rate and mean number of sucker shoot, tissue culture families were higher than normal seedlings, and then the sequence of suckering ability, CS11>CS10>CS5>CS3 was arranged. The total stem number of the clump was consistent with suckering ability among families where CS11 was the largest, CS10 and CS5 was larger, and CS3 as well as CK was the smallest, but no significant difference appeared. Mean length of mother stem and mean total length of rattan clump differed significantly at the level of 0.05 and 0.01 among families. Based on the variance analysis and multiple comparisons, two types of tissue culture families, superior type and common one were classified.
文摘The random amplified polymorphic DNA （RAPD） molecular marker technique was used to determine the sex of Calamus simplicifolius C. F. Wei In the present study, DNA samples were extracted individually from 10 male and 10 female plants. After a total of 1 040 decamer primers had been tested, an approximate 500-bp male-specific DNA fragment was generated with the S 1443 primer. It is feasible to identify sex at the early stages of plant life, which is beneficial for improving breeding programs of this dioecious species. In addition, we have obtained a proper RAPD protocol that is useful for other species of rattan.
