Anacardium occidentale L., Anacardiaceae, is frequently used to treat infections. We evaluated the antimicrobial and antioxidant activity of extracts obtained from aerial parts of the plant in comparison with the extr...Anacardium occidentale L., Anacardiaceae, is frequently used to treat infections. We evaluated the antimicrobial and antioxidant activity of extracts obtained from aerial parts of the plant in comparison with the extract prepared with the flowers. Ethanol extracts of the leaves (EEL), stem bark (EEB), and flowers (EEF) were prepared separately. The antimicrobial activity was evaluated by agar diffusion and broth dilution methods. The minimum bactericidal and fungicidal concentrations were determined against: Streptococcus mutans, Lactobacillus acidophilus, Staphylococcus aureus, MRSA, Enterococcus faecalis, Streptococcus pyogenes, Pseudomonas aeruginosa, Proteus mirabilis, Escherichia coli, Klebsiella pneumoniae, Helicobacter pylori, Salmonella choleraesuis, Candida albicans, and Candida tropicalis. The antioxidant activity of the extracts was evaluated based on their scavenger capacity of 2,2-diphenyl-1-picrylhydrazyle. The phytochemical profile was analyzed by colorimetric methods and by HPLC with UV detection. All extracts exhibited antioxidant and antimicrobial activity. Although, the EEF was the most effective since it inhibited the growth of all 14 microorganisms tested. Anacardic and galic acids were identified in all extracts as well phenolic compounds, triterpenes, flavones and xanthones. In conclusion, the extracts obtained from the aerial parts of A. occidentale, mainly the extract of flowers that was the most effective, are rich in bioactive metabolites that exert a potent antioxidant and antimicrobial effect. Taken together, the results indicate an important biotechnological potential of A. occidentale as a source of compounds with broad-spectrum antimicrobial and of antioxidant activity to be used in the food and cosmetics industry.展开更多
Coir pith, a byproduct of coconut husk is difficult to decompose due to its high lignin and cellulose content. In this study, coir pith was composted with different agro industrial by products such as cow dung, vegeta...Coir pith, a byproduct of coconut husk is difficult to decompose due to its high lignin and cellulose content. In this study, coir pith was composted with different agro industrial by products such as cow dung, vegetable market waste, poultry waste and microbial consortium. The different treatment combinations used in the present study were Control, T1 (Coir pith + Cow dung + Vegetable market waste + Poultry waste + mixed microbial culture (Trichoderma viridae + Pleurotus sajar caju), T2 (Coir pith + Vegetable market waste + Poultry waste + Tank slit + Mixed microbial culture) and T3 (Coir pith + Cow dung + poultry waste + tank slit + mixed microbial culture). At the end of 12th week, in the treatment T1, C: N ratio of 21.8:1 was observed in the composted coir pith sample. Highest P content of 0.47% and K content of 1.2% and the least Cellulose and Lignin contents of 22.8% and 10.03% were recorded in the T1 treatment after a composting period of 12 weeks. Highest pH of 7.4 was observed in the treatment T3, this was followed by T1 (7.2) treatment.展开更多
The protoplasts of auxotrophic mutant monokaryon of Pleurotus. sajor-caju was used as recipient parenL the protoplasts of wild dikareon of Lentinus edodes were heat-inactivated and used as nuclear donor parent. The fu...The protoplasts of auxotrophic mutant monokaryon of Pleurotus. sajor-caju was used as recipient parenL the protoplasts of wild dikareon of Lentinus edodes were heat-inactivated and used as nuclear donor parent. The fusants of two parents showed some their individual characteristics and physiological difference between themselfs. Thrugh protoplast re-isolation and reversion of one randomly selective fusant, two parents had been recovered and the donor parent achieved some new physiological characteristics such as faster mycelial growth and earier fruiting.This experiment result showed that the idea and the method of polarized protoplast fusion used in this study may be used as routine procedure for strain improvement in adible fungi.展开更多
文摘Anacardium occidentale L., Anacardiaceae, is frequently used to treat infections. We evaluated the antimicrobial and antioxidant activity of extracts obtained from aerial parts of the plant in comparison with the extract prepared with the flowers. Ethanol extracts of the leaves (EEL), stem bark (EEB), and flowers (EEF) were prepared separately. The antimicrobial activity was evaluated by agar diffusion and broth dilution methods. The minimum bactericidal and fungicidal concentrations were determined against: Streptococcus mutans, Lactobacillus acidophilus, Staphylococcus aureus, MRSA, Enterococcus faecalis, Streptococcus pyogenes, Pseudomonas aeruginosa, Proteus mirabilis, Escherichia coli, Klebsiella pneumoniae, Helicobacter pylori, Salmonella choleraesuis, Candida albicans, and Candida tropicalis. The antioxidant activity of the extracts was evaluated based on their scavenger capacity of 2,2-diphenyl-1-picrylhydrazyle. The phytochemical profile was analyzed by colorimetric methods and by HPLC with UV detection. All extracts exhibited antioxidant and antimicrobial activity. Although, the EEF was the most effective since it inhibited the growth of all 14 microorganisms tested. Anacardic and galic acids were identified in all extracts as well phenolic compounds, triterpenes, flavones and xanthones. In conclusion, the extracts obtained from the aerial parts of A. occidentale, mainly the extract of flowers that was the most effective, are rich in bioactive metabolites that exert a potent antioxidant and antimicrobial effect. Taken together, the results indicate an important biotechnological potential of A. occidentale as a source of compounds with broad-spectrum antimicrobial and of antioxidant activity to be used in the food and cosmetics industry.
文摘Coir pith, a byproduct of coconut husk is difficult to decompose due to its high lignin and cellulose content. In this study, coir pith was composted with different agro industrial by products such as cow dung, vegetable market waste, poultry waste and microbial consortium. The different treatment combinations used in the present study were Control, T1 (Coir pith + Cow dung + Vegetable market waste + Poultry waste + mixed microbial culture (Trichoderma viridae + Pleurotus sajar caju), T2 (Coir pith + Vegetable market waste + Poultry waste + Tank slit + Mixed microbial culture) and T3 (Coir pith + Cow dung + poultry waste + tank slit + mixed microbial culture). At the end of 12th week, in the treatment T1, C: N ratio of 21.8:1 was observed in the composted coir pith sample. Highest P content of 0.47% and K content of 1.2% and the least Cellulose and Lignin contents of 22.8% and 10.03% were recorded in the T1 treatment after a composting period of 12 weeks. Highest pH of 7.4 was observed in the treatment T3, this was followed by T1 (7.2) treatment.
文摘The protoplasts of auxotrophic mutant monokaryon of Pleurotus. sajor-caju was used as recipient parenL the protoplasts of wild dikareon of Lentinus edodes were heat-inactivated and used as nuclear donor parent. The fusants of two parents showed some their individual characteristics and physiological difference between themselfs. Thrugh protoplast re-isolation and reversion of one randomly selective fusant, two parents had been recovered and the donor parent achieved some new physiological characteristics such as faster mycelial growth and earier fruiting.This experiment result showed that the idea and the method of polarized protoplast fusion used in this study may be used as routine procedure for strain improvement in adible fungi.
