Biliary dyskinesia is a relatively common gastrointestinal disease that is increas-ing in incidence as living standards improve.However,its underlying pathogenesis remains unclear,hindering the development of therapeu...Biliary dyskinesia is a relatively common gastrointestinal disease that is increas-ing in incidence as living standards improve.However,its underlying pathogenesis remains unclear,hindering the development of therapeutic drugs.Recently,“Expression and functional study of cholecystokinin-A receptors on the interstitial Cajal-like cells of the guinea pig common bile duct”demonstrated that cholecystokinin(CCK)regulates the contractile function of the common bile duct through interaction with the CCK-A receptor in interstitial Cajal-like cells,contributing to improving the academic understanding of biliary tract dynamics and providing emerging directions for the pathogenesis and clinical management of biliary dyskinesia.This letter provides a brief overview of the role of CCK and CCK-A receptors in biliary dyskinesia from the perspective of animal experiments and clinical studies,and discusses prospects and challenges for the clinical application of CCK and CCK-A receptors as potential therapeutic targets.展开更多
BACKGROUND Many studies have shown that interstitial Cajal-like cell(ICLC)abnormalities are closely related to a variety of dynamic gastrointestinal disorders.ICLCs are pacemaker cells for gastrointestinal movement an...BACKGROUND Many studies have shown that interstitial Cajal-like cell(ICLC)abnormalities are closely related to a variety of dynamic gastrointestinal disorders.ICLCs are pacemaker cells for gastrointestinal movement and are involved in the transmission of nerve impulses.AIM To elucidate the expression profile and significance of cholecystokinin-A(CCK-A)receptors in ICLCs in the common bile duct(CBD),as well as the role of CCK in regulating CBD motility through CCK-A receptors on CBD ICLCs.METHODS The levels of tyrosine kinase receptor(c-kit)and CCK-A receptors in CBD tissues and isolated CBD cells were quantified using the double immunofluorescence labeling technique.The CCK-mediated enhancement of the movement of CBD muscle strips through CBD ICLCs was observed by a muscle strip contraction test.RESULTS Immunofluorescence showed co-expression of c-kit and CCK-A receptors in the CBD muscularis layer.Observations of isolated CBD cells showed that c-kit was expressed on the surface of ICLCs,the cell body and synapse were colored and polygonal,and some cells presented protrusions and formed networks adjacent to the CBD while others formed filaments at the synaptic terminals of local cells.CCK-A receptors were also expressed on CBD ICLCs.At concentrations ranging from 10^(-6) mol/L to 10^(-10) mol/L,CCK promoted CBD smooth muscle contractility in a dose-dependent manner.In contrast,after ICLC removal,the contractility mediated by CCK in CBD smooth muscle decreased.CONCLUSION CCK-A receptors are highly expressed on CBD ICLCs,and CCK may regulate CBD motility through the CCK-A receptors on ICLCs.展开更多
BACKGROUND Loss and/or dysfunction of interstitial Cajal-like cells(ICLCs)in the gallbladder may promote cholesterol gallstone formation by decreasing gallbladder motility.AIM To study the effect of cholesterol on the...BACKGROUND Loss and/or dysfunction of interstitial Cajal-like cells(ICLCs)in the gallbladder may promote cholesterol gallstone formation by decreasing gallbladder motility.AIM To study the effect of cholesterol on the proliferation and apoptosis of ICLCs from guinea pig gallbladders.METHODS Guinea pig gallbladder ICLCs were isolated and cultured in vitro.The cells were exposed to cholesterol solutions at different concentrations(0,25,50,and 100 mg/L)for 24 h.Then,cell proliferation was detected by the CCK-8 method and the apoptosis rate was detected by flow cytometry.Further,the expression of the c-Kit protein was detected by Western blot and the expression level of c-Kit m RNA in the cells was detected by real-time quantitative PCR.RESULTS After ICLCs were cultured with cholesterol at concentrations of 25,50,and 100 mg/L,the proliferation rates decreased significantly(P<0.05),whereas the apoptosis rates increased significantly(P<0.05).Moreover,the expression of cKit protein and m RNA decreased significantly(P<0.05).CONCLUSION High cholesterol concentrations can inhibit the proliferation of ICLCs and promote apoptosis.This decrease in the ICLC proliferation rate might be caused by the inhibition of the stem cell factor/c-Kit signaling pathway.展开更多
with a complex and multifactorial etiology.Declined gallbladder motility reportedly contributes to CG pathogenesis.Furthermore,interstitial Cajal-like cells(ICLCs)are reportedly present in human and guinea pig gallbla...with a complex and multifactorial etiology.Declined gallbladder motility reportedly contributes to CG pathogenesis.Furthermore,interstitial Cajal-like cells(ICLCs)are reportedly present in human and guinea pig gallbladder tissue.ICLCs potentially contribute to the regulation of gallbladder motility,and aberrant conditions involving the loss of ICLCs and/or a reduction in its pacing potential and reactivity to cholecystokinin may promote CG pathogenesis.This review discusses the association between ICLCs and CG pathogenesis and provides a basis for further studies on the functions of ICLCs and the etiologies of CG.展开更多
Interstitial Cajal-like cells(ICLC) have been found in the atrium,ventricle and pulmonary vein in human and anmials.ICLC with very long processes and space structure,Cell processes establish close spatial relationship...Interstitial Cajal-like cells(ICLC) have been found in the atrium,ventricle and pulmonary vein in human and anmials.ICLC with very long processes and space structure,Cell processes establish close spatial relationships between each other, as well as with cardiomyocyte and nerve endings.ICLC appear located among the myocardial cells and particularly at the border between the myocardial sleeve and pulmonary vein wall.ICLC seems to possess automaticity and triggered activity.ICLC is a potential substrate for arrhythmia.展开更多
文摘Biliary dyskinesia is a relatively common gastrointestinal disease that is increas-ing in incidence as living standards improve.However,its underlying pathogenesis remains unclear,hindering the development of therapeutic drugs.Recently,“Expression and functional study of cholecystokinin-A receptors on the interstitial Cajal-like cells of the guinea pig common bile duct”demonstrated that cholecystokinin(CCK)regulates the contractile function of the common bile duct through interaction with the CCK-A receptor in interstitial Cajal-like cells,contributing to improving the academic understanding of biliary tract dynamics and providing emerging directions for the pathogenesis and clinical management of biliary dyskinesia.This letter provides a brief overview of the role of CCK and CCK-A receptors in biliary dyskinesia from the perspective of animal experiments and clinical studies,and discusses prospects and challenges for the clinical application of CCK and CCK-A receptors as potential therapeutic targets.
文摘BACKGROUND Many studies have shown that interstitial Cajal-like cell(ICLC)abnormalities are closely related to a variety of dynamic gastrointestinal disorders.ICLCs are pacemaker cells for gastrointestinal movement and are involved in the transmission of nerve impulses.AIM To elucidate the expression profile and significance of cholecystokinin-A(CCK-A)receptors in ICLCs in the common bile duct(CBD),as well as the role of CCK in regulating CBD motility through CCK-A receptors on CBD ICLCs.METHODS The levels of tyrosine kinase receptor(c-kit)and CCK-A receptors in CBD tissues and isolated CBD cells were quantified using the double immunofluorescence labeling technique.The CCK-mediated enhancement of the movement of CBD muscle strips through CBD ICLCs was observed by a muscle strip contraction test.RESULTS Immunofluorescence showed co-expression of c-kit and CCK-A receptors in the CBD muscularis layer.Observations of isolated CBD cells showed that c-kit was expressed on the surface of ICLCs,the cell body and synapse were colored and polygonal,and some cells presented protrusions and formed networks adjacent to the CBD while others formed filaments at the synaptic terminals of local cells.CCK-A receptors were also expressed on CBD ICLCs.At concentrations ranging from 10^(-6) mol/L to 10^(-10) mol/L,CCK promoted CBD smooth muscle contractility in a dose-dependent manner.In contrast,after ICLC removal,the contractility mediated by CCK in CBD smooth muscle decreased.CONCLUSION CCK-A receptors are highly expressed on CBD ICLCs,and CCK may regulate CBD motility through the CCK-A receptors on ICLCs.
基金Supported by the National Natural Science Foundation of China,No.81000183the Natural Science Foundation of Liaoning Province,No.20180550125。
文摘BACKGROUND Loss and/or dysfunction of interstitial Cajal-like cells(ICLCs)in the gallbladder may promote cholesterol gallstone formation by decreasing gallbladder motility.AIM To study the effect of cholesterol on the proliferation and apoptosis of ICLCs from guinea pig gallbladders.METHODS Guinea pig gallbladder ICLCs were isolated and cultured in vitro.The cells were exposed to cholesterol solutions at different concentrations(0,25,50,and 100 mg/L)for 24 h.Then,cell proliferation was detected by the CCK-8 method and the apoptosis rate was detected by flow cytometry.Further,the expression of the c-Kit protein was detected by Western blot and the expression level of c-Kit m RNA in the cells was detected by real-time quantitative PCR.RESULTS After ICLCs were cultured with cholesterol at concentrations of 25,50,and 100 mg/L,the proliferation rates decreased significantly(P<0.05),whereas the apoptosis rates increased significantly(P<0.05).Moreover,the expression of cKit protein and m RNA decreased significantly(P<0.05).CONCLUSION High cholesterol concentrations can inhibit the proliferation of ICLCs and promote apoptosis.This decrease in the ICLC proliferation rate might be caused by the inhibition of the stem cell factor/c-Kit signaling pathway.
基金National Natural Science Foundation of China,No.81000183and Natural Science Foundation of Liaoning Province,No.20180550125.
文摘with a complex and multifactorial etiology.Declined gallbladder motility reportedly contributes to CG pathogenesis.Furthermore,interstitial Cajal-like cells(ICLCs)are reportedly present in human and guinea pig gallbladder tissue.ICLCs potentially contribute to the regulation of gallbladder motility,and aberrant conditions involving the loss of ICLCs and/or a reduction in its pacing potential and reactivity to cholecystokinin may promote CG pathogenesis.This review discusses the association between ICLCs and CG pathogenesis and provides a basis for further studies on the functions of ICLCs and the etiologies of CG.
文摘Interstitial Cajal-like cells(ICLC) have been found in the atrium,ventricle and pulmonary vein in human and anmials.ICLC with very long processes and space structure,Cell processes establish close spatial relationships between each other, as well as with cardiomyocyte and nerve endings.ICLC appear located among the myocardial cells and particularly at the border between the myocardial sleeve and pulmonary vein wall.ICLC seems to possess automaticity and triggered activity.ICLC is a potential substrate for arrhythmia.
