Pentachlorophenol（PCP）, a priority pollutant due to its persistence and high toxicity, has been used worldwide as a pesticide and biocide. To understand the adverse effects of PCP, adult male white-rumped munias（Lo...Pentachlorophenol（PCP）, a priority pollutant due to its persistence and high toxicity, has been used worldwide as a pesticide and biocide. To understand the adverse effects of PCP, adult male white-rumped munias（Lonchura striata） were orally administrated commercial PCP mixed with corn oil at dosages of 0, 0.05, 0.5, and 5 mg/（kg·day） for 42 day. Gas chromatography–mass spectrometry（GC–MS） analysis found that PCP was preferentially accumulated in the kidney rather than in the liver and muscle in all exposure groups. To examine the function of CYP1A in pollutant metabolism, we isolated two full-length cDNA fragments（designated as CYP1A4 and CYP1A5） from L. striata liver using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. PCP induced the expression of CYP1A5, although no obvious change was observed in CYP1A4 expression. Furthermore, PCP significantly elevated the activities of ethoxyresorufin O-deethylase and methoxyresorufin O-demethylase and decreased the activity of benzyloxy-trifluoromethyl-coumarin, with no significant responses observed in benzyloxyresorufin O-debenzylase. PCP induced significant changes in antioxidant enzyme（superoxide dismutase and catalase） activities and malondialdehyde content, but decreased glutathione peroxidase（GSH-Px） and glutathione S-transferase activities and GSH content in the liver of L. striata. The present study demonstrated that PCP had hepatic toxic effects by affecting CYP1As and anti-oxidative status.展开更多
AIM: To investigate CYP2E1 IgG4 autoantibody levels and liver biochemical markers in adult patients after anesthesia with desflurane. METHODS: Forty patients who were > 18 years old and undergoing elective surgery ...AIM: To investigate CYP2E1 IgG4 autoantibody levels and liver biochemical markers in adult patients after anesthesia with desflurane. METHODS: Forty patients who were > 18 years old and undergoing elective surgery under general anes-thesia with desflurane were studied. Alpha-glutathione-S-transferase(aGST) and IgG4 antibodies againstCYP2E1 were measured preoperatively and 96 h post-operatively, as well as complete blood count, prothrom-bin time(PT), activated partial thromboplastin time(aPTT), international normalized ratio(INR), aspartate aminotransferase(SGOT), alanine aminotransferase(SGPT), g-glutamyl-transpeptidase(gGT), alkaline phosphatase, total serum proteins, albumin and bili-rubin. A separate group of 8 patients who received re-gional anesthesia was also studied for calibration of the methodology used for CYP2E1 IgG4 and aGST measure-ments. Student's t-test and the Mann-Whitney U test were used for comparison of the continuous variables, and Fisher's exact test was used for the categorical variables. All tests were two-tailed, with statistical sig-nificance set as P < 0.05.RESULTS: None of the patients developed postopera-tive liver dysfunction, and all patients were successfully discharged from the hospital. No statistically significant difference was observed regarding liver function tests(SGOT, SGPT, γGT, bilirubin, INR), aGST and CYP2E1 IgG4, before and after exposure to desflurane. After dividing patients into two subgroups based on whether or not they had received general anesthesia in the past, no significant difference in the levels of CYP2E1 IgG4 was observed at baseline or 96 h after desflurane administration(P = 0.099 and P = 0.051, respectively). Alpha-GST baseline levels and levels after the interven-tion also did not differ significantly between these two subgroups(P > 0.1). The mean aGST differences were statistically elevated in men by 2.15 ng/mL compared to women when adjusted for BMI, duration of anesthe-sia, number of times anesthesia was administered pre-viously and length of hospi展开更多
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDB14040202)the National Natural Science Foundation of China (Nos. 31320103915 and 21377128)
文摘Pentachlorophenol（PCP）, a priority pollutant due to its persistence and high toxicity, has been used worldwide as a pesticide and biocide. To understand the adverse effects of PCP, adult male white-rumped munias（Lonchura striata） were orally administrated commercial PCP mixed with corn oil at dosages of 0, 0.05, 0.5, and 5 mg/（kg·day） for 42 day. Gas chromatography–mass spectrometry（GC–MS） analysis found that PCP was preferentially accumulated in the kidney rather than in the liver and muscle in all exposure groups. To examine the function of CYP1A in pollutant metabolism, we isolated two full-length cDNA fragments（designated as CYP1A4 and CYP1A5） from L. striata liver using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. PCP induced the expression of CYP1A5, although no obvious change was observed in CYP1A4 expression. Furthermore, PCP significantly elevated the activities of ethoxyresorufin O-deethylase and methoxyresorufin O-demethylase and decreased the activity of benzyloxy-trifluoromethyl-coumarin, with no significant responses observed in benzyloxyresorufin O-debenzylase. PCP induced significant changes in antioxidant enzyme（superoxide dismutase and catalase） activities and malondialdehyde content, but decreased glutathione peroxidase（GSH-Px） and glutathione S-transferase activities and GSH content in the liver of L. striata. The present study demonstrated that PCP had hepatic toxic effects by affecting CYP1As and anti-oxidative status.
文摘AIM: To investigate CYP2E1 IgG4 autoantibody levels and liver biochemical markers in adult patients after anesthesia with desflurane. METHODS: Forty patients who were > 18 years old and undergoing elective surgery under general anes-thesia with desflurane were studied. Alpha-glutathione-S-transferase(aGST) and IgG4 antibodies againstCYP2E1 were measured preoperatively and 96 h post-operatively, as well as complete blood count, prothrom-bin time(PT), activated partial thromboplastin time(aPTT), international normalized ratio(INR), aspartate aminotransferase(SGOT), alanine aminotransferase(SGPT), g-glutamyl-transpeptidase(gGT), alkaline phosphatase, total serum proteins, albumin and bili-rubin. A separate group of 8 patients who received re-gional anesthesia was also studied for calibration of the methodology used for CYP2E1 IgG4 and aGST measure-ments. Student's t-test and the Mann-Whitney U test were used for comparison of the continuous variables, and Fisher's exact test was used for the categorical variables. All tests were two-tailed, with statistical sig-nificance set as P < 0.05.RESULTS: None of the patients developed postopera-tive liver dysfunction, and all patients were successfully discharged from the hospital. No statistically significant difference was observed regarding liver function tests(SGOT, SGPT, γGT, bilirubin, INR), aGST and CYP2E1 IgG4, before and after exposure to desflurane. After dividing patients into two subgroups based on whether or not they had received general anesthesia in the past, no significant difference in the levels of CYP2E1 IgG4 was observed at baseline or 96 h after desflurane administration(P = 0.099 and P = 0.051, respectively). Alpha-GST baseline levels and levels after the interven-tion also did not differ significantly between these two subgroups(P > 0.1). The mean aGST differences were statistically elevated in men by 2.15 ng/mL compared to women when adjusted for BMI, duration of anesthe-sia, number of times anesthesia was administered pre-viously and length of hospi
