Objective To investigate CTLA4-Ig's potential role in therapy for allergic asthma by blocking B7/CD28 interactions with cytotoxic T lymphocyte antigen 4-Ig (CTLA4-Ig).Methods We divided BALB/C mice into the three ...Objective To investigate CTLA4-Ig's potential role in therapy for allergic asthma by blocking B7/CD28 interactions with cytotoxic T lymphocyte antigen 4-Ig (CTLA4-Ig).Methods We divided BALB/C mice into the three groups: Sham/Sham (control), ovalbumin (OVA)/OVA and mCTLA4-Ig. Blood, bronchoalveolar lavage, histology and determination of cytokines were performed 24 hours after airway challenge. Results In the OVA/OVA group, the number of cells, the percentage of inflamed cells and the level of IL-4 in BALF were increased. Airways in our murine model for allergic asthma underwent pathological changes, which were significantly reduced after treatment with mCTLA4-Ig. Conclusion Blockage of co-stimulation with mCTLA4-Ig can inhibit allergy-specific response of T cells, and asthmatic response as well.展开更多
目的探讨细胞毒性T淋巴细胞相关抗原4免疫球蛋白(Cytotoxic T Lymphocyte Antigen4-immunoglobulin,CTLA4-Ig)与髓腔内骨髓移植(IBM-BMT)联合应用对诱导供体小鼠皮肤移植免疫耐受的促进bd作用。方法受鼠为C57BL/6(H-2,B6)雌性小鼠,于第...目的探讨细胞毒性T淋巴细胞相关抗原4免疫球蛋白(Cytotoxic T Lymphocyte Antigen4-immunoglobulin,CTLA4-Ig)与髓腔内骨髓移植(IBM-BMT)联合应用对诱导供体小鼠皮肤移植免疫耐受的促进bd作用。方法受鼠为C57BL/6(H-2,B6)雌性小鼠,于第0天输注雄性BALB/c(2)小鼠来源的骨髓细胞(BMC),同时腹腔注射CTLA4-Ig;第6天进行皮肤移植,并于移植术后第2天再进行一次腹腔注射CTLA4-Ig,观察皮肤移植物存活时间。通过皮肤移植物的组织学分析,以及混合淋巴细胞反应(MLR)检测耐受状态,并通过骨髓染色体分析了解BMT的植入程度。结果联合治疗组B6小鼠的皮肤移植物平均存活时间(MST)显著长于其它各组(P<0.05);MLR结果证明,联合组B6小鼠获得供体特异性耐受,在耐受小鼠骨髓中可检测到一定比例的Y染色体存在。结论 CTLA4-Ig与髓腔内骨髓移植可保证异基因的骨髓细胞形成嵌合体,显著延长了移植物存活时间,诱导免疫耐受。展开更多
目的:探讨细胞毒性T淋巴细胞相关抗原4免疫球蛋白(Cytotoxic T Lymphocyte Antigen 4-i mmunoglobulin,CTLA4-Ig)与供体骨髓细胞联合应用诱导器官移植免疫耐受的可行性。方法:应用小鼠腹部心脏移植模型,实验组小鼠术中输注供体骨髓细胞...目的:探讨细胞毒性T淋巴细胞相关抗原4免疫球蛋白(Cytotoxic T Lymphocyte Antigen 4-i mmunoglobulin,CTLA4-Ig)与供体骨髓细胞联合应用诱导器官移植免疫耐受的可行性。方法:应用小鼠腹部心脏移植模型,实验组小鼠术中输注供体骨髓细胞,术后腹腔注射CTLA4-Ig。观察移植心脏存活时间,并进行病理分析,检测迟发性超敏反应(Delayed Type Hyper-sensitivity,DTH)。后期进行供体来源的皮肤移植并观察存活时间。结果:实验组移植心脏存活时间较对照组显著延长,达(79.3±23.7)d;排斥反应减轻,并产生了供体抗原特异性的免疫低反应性;后期进行的皮肤移植实验中,供体来源的皮肤存活时间也显著延长,达(23.0±3.1)d。结论:CTLA4-Ig与骨髓细胞联合应用可以显著延长移植物存活时间,诱导实体器官移植免疫耐受。两者具有明显的协同作用。展开更多
文摘Objective To investigate CTLA4-Ig's potential role in therapy for allergic asthma by blocking B7/CD28 interactions with cytotoxic T lymphocyte antigen 4-Ig (CTLA4-Ig).Methods We divided BALB/C mice into the three groups: Sham/Sham (control), ovalbumin (OVA)/OVA and mCTLA4-Ig. Blood, bronchoalveolar lavage, histology and determination of cytokines were performed 24 hours after airway challenge. Results In the OVA/OVA group, the number of cells, the percentage of inflamed cells and the level of IL-4 in BALF were increased. Airways in our murine model for allergic asthma underwent pathological changes, which were significantly reduced after treatment with mCTLA4-Ig. Conclusion Blockage of co-stimulation with mCTLA4-Ig can inhibit allergy-specific response of T cells, and asthmatic response as well.
文摘目的探讨细胞毒性T淋巴细胞相关抗原4免疫球蛋白(Cytotoxic T Lymphocyte Antigen4-immunoglobulin,CTLA4-Ig)与髓腔内骨髓移植(IBM-BMT)联合应用对诱导供体小鼠皮肤移植免疫耐受的促进bd作用。方法受鼠为C57BL/6(H-2,B6)雌性小鼠,于第0天输注雄性BALB/c(2)小鼠来源的骨髓细胞(BMC),同时腹腔注射CTLA4-Ig;第6天进行皮肤移植,并于移植术后第2天再进行一次腹腔注射CTLA4-Ig,观察皮肤移植物存活时间。通过皮肤移植物的组织学分析,以及混合淋巴细胞反应(MLR)检测耐受状态,并通过骨髓染色体分析了解BMT的植入程度。结果联合治疗组B6小鼠的皮肤移植物平均存活时间(MST)显著长于其它各组(P<0.05);MLR结果证明,联合组B6小鼠获得供体特异性耐受,在耐受小鼠骨髓中可检测到一定比例的Y染色体存在。结论 CTLA4-Ig与髓腔内骨髓移植可保证异基因的骨髓细胞形成嵌合体,显著延长了移植物存活时间,诱导免疫耐受。
文摘目的:探讨细胞毒性T淋巴细胞相关抗原4免疫球蛋白(Cytotoxic T Lymphocyte Antigen 4-i mmunoglobulin,CTLA4-Ig)与供体骨髓细胞联合应用诱导器官移植免疫耐受的可行性。方法:应用小鼠腹部心脏移植模型,实验组小鼠术中输注供体骨髓细胞,术后腹腔注射CTLA4-Ig。观察移植心脏存活时间,并进行病理分析,检测迟发性超敏反应(Delayed Type Hyper-sensitivity,DTH)。后期进行供体来源的皮肤移植并观察存活时间。结果:实验组移植心脏存活时间较对照组显著延长,达(79.3±23.7)d;排斥反应减轻,并产生了供体抗原特异性的免疫低反应性;后期进行的皮肤移植实验中,供体来源的皮肤存活时间也显著延长,达(23.0±3.1)d。结论:CTLA4-Ig与骨髓细胞联合应用可以显著延长移植物存活时间,诱导实体器官移植免疫耐受。两者具有明显的协同作用。
